为了研究电针镇痛过程中脑内乙酰胆碱(ACh)代谢的动态变化规律

本文将用更新率的研究方法

从电针镇痛过程中脑内ACh含量的变化

电针对脑内ACh合成率的影响和电针对ACh利用率的影响等三个方面作一探讨。一、电针镇痛过程中脑内ACh含量的变化In order to further evaluate the role of central cholinergic nerves in acupuncture analgesia

the effect of acupuncture on the content and turnover rate of central ACh in rats was examined in the present study. The pain threshold after 30 minutes' electroacupuncture in rats (14) (0.110 mA) is significantly higher than that in the control rats (14) (0.044mA ) (P<0.01); and the ACh content in brain of the electroacupuncture group is also obviously elevated

i.e. in caudate nucleus

2.022 to 2.455 r/g (P<0.05)

and hypothalamus

1.572 to 1.927r/g.Hemicholine and eserine were used as tools to block the synthesis and the degradation of ACh respectively for the purpose of the assessment of the utilizing and synthesizing rate of ACh. It was found that during acupuncture analgesia the utilization rate of ACh was significantly accelerated in brain of rats(103); in caudate nucleus the rate increased in electroacupuncture group twice in the control

and in hypothalamus

it is also nearly doubled. In addition

the rate of synthesis was also in high speed ( rats 63). Present studies of turn over rate indicated that in central nervous system the elevation of ACh content by electroacupuncture results from the new kinetics balance between the increased rates of ACh utilization and synthesis. In combination with reports available on this subject it may be suggested that the increase of utilization rate present an evidence that ACh may play an important role in electroacupuncture analgesia.