过去我们以血浆中促使去甲肾上腺素(NA)合成酶——多巴胺β羟化酶(DβH)活性

作为交感神经活动的指标

曾观察了交感神经活动与针麻效果的关系

针麻效果好者

DβH活性不变或略为降低

提示针刺能使交感神经在手术创伤的强烈刺激下保持相对稳定的状态。但是

DβH活性的高低只能间接反映血浆中NA的水平

而直接测定血浆中NA水平才能更直接地反映交感神经活动状态。同时既观察DβH活性

又测定血浆NA的水平

从两者的变化关系

更能Our previous clinical practice showed that acupuncture might stabilize the sympathetic nervous function. The changes of plasma DβH activity was in a lower level in grade Ⅰ cases of patients during subtotal gastre- ctomy under acupuncture anesthesia. In the present work the effect of acupuncture anesthesia on the changes of plasma NA level and DβH acti- vity was further studied on dog models during subtotal gastrectomy under acupuncture anesthesia. The experiments were divided into four groups: 1) subtotal gastrectomy was performed without acupuncture or any drug; 2) Operation was perfo- rmed under acupuncture combined with basal anesthetic drugs (Dolantine i.v. 1 mg/kg

Atropine 0.3 mg i.m. and some local infiltration with pro- caine); 3) Operation was done under acupuncture alone; 4) Acupuncture was made without operation. Blood samples were taken before operation

after induction and during operation. The plasma NA level and DβH acti- vity were determined with radio-enzymatic and spectrophotometric assay respectively. Before acupuncture NA level of dog plasma was 0.45±0.02 ng/ml

DβH activity

23.8±5.13 nm/0.2 ml/h; NA/DβH ratio was 0.0188. In the group of only acupuncture without operation

the level of plasma NA was incre- ased to 126%

DβH activity to 91.1%

and NA/DβH to 136.2% after acu- puncture as compared to before acupuncture. In the group of operation under acupuncture anesthesia

NA

DβH and NA/DβH were increased to 188%

110.8% and 155.9% respectively as compared to before acupuncture. In the group of operation without acupuncture or any drug NA

DβH

and NA/DβH were increased 305%

110%

and 197.9% respectively. The plasma NA level in the group of acupuncture anesthesia is between that of the group of operation without acupuncture or any drug and that of the group of only acupuncture without operation. It was pointed out that the regu- latory function of acupuncture could inhibit the release of NA induced by the various surgical traumatic stimulation. A positive correlation presented between the plasma DβH activity and the change of NA level (r=0.335

P<0.02). When the level of plasma NA increased the DβH activity also increased. The NA level is used as the indication of the instantaneous activity of sympathetic nerve

while the DβH activity as the indication of the long period or continuous change of the function of sympathetic nerve. It is suggest that NA level and DβH activity could be used as an index of sympathetic nervous functional state of peripheral sympathetic nerve.