姐妹染色单体交换(SCE)是检查致癌或突变因子的一种方法

此法灵敏、准确、快速。已知某些麻醉药或物理刺激可诱发突变或癌变。在研究某些作用于DNA (脱氧核糖核酸)的诱变剂或致癌物质。可在极低的

不能或很少产生染色体畸变的量就能诱发大量SCE

目前已把SCE列为检测环境中突变或癌变的可疑因子的方法

以往对于致癌物质的标准生物鉴定

大多用动物终身接触试验

但这种方法

时间漫长

人力物力浪费也很大

采用细胞遗传技术是较迅速的筛选试验

因为SCE能敏感地反映DNA的损伤和修复情况。实践证明针刺麻醉用于甲状腺手术具有The effect of acupuncture anesthesia (AA) for thyroidectomy on SCE RNA/DNA ration in lymphocytes was studied in 11 cases blood samples from peripheral vein were collected prior to AA (negative control)

3 hrs

and 6 days after AA and with mitomycin (positive control). One part of each sampl was subjected to Bre Drd-dye technique for detection of SCE

the other part for RNA/DNA ratios measured by CTAB method. From the results we may conclude that the phenomenan of mutation could not be induced by AA and SCE analysis may be considered a fairly good indicator for mutagenio activity in the study of AA. The quantitative ration of RNA/DNA in three groups were 11.033 (in pr- eanesthesia)

7.498 and 9.159 (3 hrs and 6 days post-AA

respectively). There were no significant differences amomg the RNA /DNA rations betw- een pre-AA and post-AA samples. The fact that either SGE or RNA/DNA ration did not show awy sign- ificant changes after AA provided strong support to the concept that acu- puncture is harmless in the respect of carcinogenesis.