Substance P(SP) immunoreactivity in the rat brain and spinal cord were measured by radioimmunoassay and studied to correlat with the analgesic effect induced by electroacupuncture(EA).The results showed following: (1)There was a significant elevation in SP levels s(?) the hippocampus

hypothalamus and striatum after 30min of EA.There was a markedly fall in the spinal cord.Statistical analysis revealed a positive correlrtion between the EA effect and the SP content in hypothalamus

striatum and statistical analysis revealed a correlation.In the spinal cord.(p<0.01)

while the SP content in the hippocampus exhibited a similar degree of elevation in non respon- sive and good responsive animals to EA stimulation. (2) SP levels in the hippocamus

hypothalamus and striatum were increased by electroacupuncture stimulation (3v).The frequency of 1.5Hz was no obvious difference as compared with 100Hz in the effects on brain SP content. (3)SP content in the spinal cord decreased only using clectroacupuncture stimulations of combination of higher intensity (3v) and higher frequency (100 Hz). (4) This effect could be blocked by the naloxone (i.p) and LSD (icv)

but icv injection of Met-enkephalin antibodies had no affects on them.