“子午流注”针灸疗法是中医颇具特色的治疗手段

临床实践表明疗效较好。用现代时辰生物学方法对这一独特疗法加以深入研究是有重要意义的。在许多细胞的生理活动中

cAMP

cGMP 是重要的第二信使

二者在细胞内的含量及其比例与细胞的功能状态有密切关系。已有文献报道人血浆 cAMP 水平存在近日节律性变化;另有报道表明血浆96 healthy male Wistar rats were housed in a light-controlled room for over 10 days prior to the experiment.Lights were on between 8:00 and 20:00 each day.Food and water were ad arbilrium.The animals were divided into acupuncture group and control group.Experimental times were 5:00

11:00

17:00 and 23:00.After acupuncture at bilateral Huantiao points for 30 minutes the animals were decapitated immediatly for plasma cAMP and cGMP radio- immunoassay.The results showed.1.The plasma level of cAMP presented a diurnal variation which peaked at 17:00 and (?)ouched the bottom at 23:00 Acupuncture increases the concentrations of cAMP in all four times of the day but did not alter its diurnal pattern.2.There was no significant difference among the plasma cGMP levels at the four limes of the day.Acupuncture could increase the cGMP levels at 5:00

17:00 and 23:00 while not at 11:00

this suggests that the effects of acupuncture on plasma cGMP level are different at different times of the day.