METHODS:45 rats(150-200g)were divided into 9 groups.The experimental steps as fol-lows:(1)injured left tibial nerve(group 1-8)and sham operation(group 9);(2)three days afteroperation

group 1

3

5 and 7 were stimulated with electro-acupuncture(EA)in Huantiao(+)and Sanyinjiao(-);(3)according to different survival time(group 1

2/10 days

group 3

4/15days

group 5

6/20 days

group 7

8

9/25 days)

48h before sacrifice

the left femoral nerve wascut

HRP was injected into the hypodermis of paw pad and gastrocnemius;(4)observe HRP la-belled cells in the lumber sympathetic ganglia

ganglion spinale(L4-6)and cornu ventrale ofspinal cord(L3-S3).RESULTS:EA 7 days

2-5 labeled cells were only found in ganglion spinalesof group 1;EA for 12 days

the more HRP lablled cells were observed in the lumber sympatheticganglions((?)=2.6)

ganglion spinales((?)=4.8)and cornu ventrale((?)=6)of group 3 as com-pared with group 4(P<0.05);25 days after operation

the quantity of labeled cells in group 8was close to group 9.CONCLUSION:(1)EA promote the regeneration of three or more kinds offibers in tibial nerve;(2)The regeneration velocity of sensory fibers was faster than sympatheticand somatic efferent fibers.