In this experiment

three groups were used to detect c-fos expression in theCNS in the awake male

S.D.rats

i.e

EA of “Ouanliao” (14Hz)

electrostimu-lation of the rat tail (0.4-0.6mA) and the control.Fos was identified by ABCimmunohistochemical method.The results were as follows:Ⅰ.The labelling cell-ular nuclei of pain group are in the laminae Ⅰ

Ⅲ

Ⅴ

Ⅶ

and Ⅹ of the spinal cordthe medial part of the frontal cortex (FC) and that of the EA group wrer inthe trigeminal nucleus and the lateral area of the FC.The number of thelabelling nuclei (n) of EA group war more than that of the pain group in therostral ventromedical medulla

the lateral reticular

the solitary n.the popiaqu-eductal grey

the parafacicular n

the centromedian n.and the subparafascicularn

of the thalamus.2.The labelling nuclei of EA and pain groups appear commonly in thedorsal raphe n.

the locus ceruleus

the periventricular n.of the thalamus

the hypothalamue

the lateral habenu-lar n.

and the sepatal area.In the control animals

in general

the labelled nucleiwere sparsely seen.