31 adult rats were divided into 4 groups:1)Electroacupuncture group;2)Capsaicin group(capsaicin was applied one the left sciatic nerve

the night on of the same body was used with ve-hicle as control);3)Capaicin combined with electroacupuncture group;4)Control group.Painthresholds

both basal and after treatment were determined.Successive cryo-sections of DRGwere cut and the neighbouring section of the same group were performed for AChE and FRAP ex-amination.Results:Little disparity in basal pain threshold was found among the four groups.The painthreshold became obviously higher after electroacupuncture

but only slightly higher after cap-saicin treatment combined with electroacupuncture.In the electroacupuncture group

the numberof DRG-B-type cell containing strong positive FRAP and AChE was obviously more than in thecontrol group.In the capsaicin group

the number of DRG-B-type cell with FRAP and AChE ac-tivity was obviously reduced as compared with the untreated side of the same body.In the cap-saicin and electroacupuncture group FRAP and AChE-activities in the DRG-B-type cells wereslightly increased.The results showed that strong activities of FRAP and AChE were found inpart of the DRG cells of normal rats

the two enzymes were found to coexist in the some B-typecells.The effect of electroacupuncture analgesia was depressed after capsaicin treatment

whilethe activities of the two enzymes and the number of positive cells appeared in the ganglion werereduced correspondingly.Nevertheless

under electeoacupuncture analgesia AA the enzymes ac-tiveites and the numvers of positive cells were increased.On account of DRG still contained ACh

it was suggested that FRAP and AChE positive DRG neurons have some relationship with the af-ference of pain and acupuncture sensation.That is to say that cholinergic nerves and their trans-mitter ACh may participate in the transmission of primary sensory messages and capsaicin sensi-tive c-type afferent fibers were an important component participated in AA.