The Subunit αo of guanine nucleotide-binding protein(rabbit polyclonal antiserum 9120

K

J.Chang.USA)

in the areas of rat spinal cord was localized by immunohistochemical PAP methods

and the changes of αo-immunoreactivity (αo-IR ) in the substantia gelatinosa (SG) were observed after unilateral tranartion of dorsal roots and electroacupuncture.Intense αo-IR was presented in Rexed lamina Ⅰ to Ⅲ of the dorsal horn

and the highest αo-IR in lamina(SG)

which was found than that in the normal and control animals.Iateral spinal nucleus of rat revealed higher density of αo-IR containing fiber networks.Following unilateral transection of dorsal roots and pain threshold increased significantly after electroacupuncture applied at the point "Huantiao"

αo-IR was markedly decreased than that in the normal and control group in those areas.The optic density(OD) of αo-IR were measured

which decreased more markedly in opration and electroacupuncture group than that normal and control gruoup.The results mentioned above indicate that Go-protein in terminals of primary nociceptive neurons of SG decreases markedly after dorsal roots transected and electroacupuncture.It was inferenced that partial Go-protein probably originates from the primary sensory neurons

and the release of "inhibitory"of Go may increase(with weaker αo-IR).In a work. Go might playimportant roles on the primary afferents informational regulation and electroacupuncture analgesia of the regulation