Immunohistochemical Changes of CGRP in Spinal Cord of Diabetic Rats and Therapeutic Effects of EA

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Immunohistochemical Changes of CGRP in Spinal Cord of Diabetic Rats and Therapeutic Effects of EA

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- Immunohistochemical Changes of CGRP in Spinal Cord of Diabetic Rats and Therapeutic Effects of EA
- Acupuncture Research Issue 2, Pages: 126-130(1999)
- 作者机构：
  
  1. 首都医科大学宣武医院老年病医疗研究中心
  2. 中国中医研究院针灸研究所,北京,100700
- 作者简介：
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- Published：1999
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Immunohistochemical Changes of CGRP in Spinal Cord of Diabetic Rats and Therapeutic Effects of EA[J]. Acupuncture research, 1999, (2): 126-130.
DOI：

Immunohistochemical Changes of CGRP in Spinal Cord of Diabetic Rats and Therapeutic Effects of EA[J]. Acupuncture research, 1999, (2): 126-130. DOI：

摘要

为观测糖尿病大鼠脊髓水平（背角浅层和腹角运动神经元）降钙素基因相关肽（ＣＧＲＰ）的含量及电针的治疗效应，采用免疫组织化学方法结合图像分析。结果：①造模三个月的糖尿病（ＤＭ）组脊髓背角浅层ＣＧＲＰ的相对含量与正常（ＮＤ）组相比显著降低（Ｐ＜０．００１）；经电针治疗后ＣＧＲＰ含量与ＮＤ组比较虽也下降（Ｐ＜０．０１或０．００１），但与ＤＭ组相比明显减轻，有极显著差异（Ｐ＜０．００１）。造模六个月的ＤＭ组及电针治疗组的情形与造模三个月时相似。②造模三个月的ＤＭ组与ＮＤ组相比，脊髓腹角ＣＧＲＰ阳性运动神经元所占百分比显著减少，强度明显减弱（Ｐ均＜０．００１）。经电针治疗后ＣＧＲＰ阳性运动神经元所占百分比与ＮＤ组相似（Ｐ＞０．０５），但强度稍弱（Ｐ＜０．０５）。结论：糖尿病大鼠脊髓背角浅层及腹角运动神经元ＣＧＲＰ含量均下降，电针治疗对这些变化具有一定的防治作用。

Abstract

Immunohistochemical methods combined with computer image pattern analysis revealed that

(1 ) the relative content of CGRP in the superficial layer of spinal dorsal horn was 70. 35 ± 1. 29 in age matched (ND) group and 45. 08 ± 1. 35 in diabetic mellitus (DM) group modellized for 3 months

dropped significantly (P < 0. 001)

which of electroacupuncture (EA) group was 58. 48 ± 1. 11

elevated significantly compared with DM group (P < 0. 001 ). The content of CGRP of DM group modellized for 6 months was similiar with that for 3 months; (2) the percentage and the intensity of CGRPLI positive neurons in spinal ventral horn of DM group for 3 months dropped significantly respectively compared with that of ND group (P < 0. 001 )

but the percentage of CGRP-LI positive neurons in EA group was similiar with (P >0. 05 )

and the staining intensity in EA group was weaker than (P < 0. 05 ) that of age matched group. These results indicate that CGRP contents in the superficial layer of spinal dorsal horn and ventral horn decrease in DM group

and EA can prevent it effectively.

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