目的 :观察电针“足三里”、“三阴交”穴对胃酸分泌的影响及血浆、胃液GAS、EGF的变化

比较阴阳经穴对脏腑功能影响的差异关系

探讨电针调节胃酸分泌的机制。方法 :75只SD大鼠随机分为空白对照组、非经非穴组、“足三里”穴组、“三阴交”穴组和联合穴位组

测定空腹胃液量、胃液 pH值及胃液酸度

同时放免法测定血浆和胃液胃泌素 (GAS)、表皮生长因子 (EGF)含量。结果 :电针后“足三里”穴组和“三阴交”穴组胃液量均显著减少 (P <0 .0 1

0 .3 0± 0 .1 1mL

0 .43± 0 .0 7mL

与 0 .63± 0 .1 2mL对照 )

胃液 pH值变化不大

前者胃液酸度明显下降 (P <0 .0 5

3 0 .5± 3 .3mmol/L

与 40 .9± 8.9mmol/L对照 )

后者无变化。二穴联合酸度显著下降 (P <0 .0 1

2 8.3± 4.5mmol/L

与 40 .8± 8.9mmol/L对照 )。“足三里”穴组胃液胃泌素显著下降 (P <0 .0 5

2 72 .6± 60 .8ng/L

与 2 84.3± 3 1 .9ng/L对照 )

EGF显著升高 (P <0 .0 1

3 .2 2± 1 .1 μg/L

与 1 .8± 0 .4μg/L对照 )

“三阴交”穴组胃液GAS升高 (P <0 .0 1

453 .9± 8.1 1ng/L

与 2 84.3± 3 1 .9ng/L对照 )

胃液EGF也升高 (P <0 .0 1

2 .6± 0 .2 μg/L

与 1 .8± 0 .4μg/L对照 )

各组胃液EGF和GAS均无相关性

“Objective: To explore the regulative effect of electroacupuncture(EA) on gastric acid secretion and its mechanisms. Methods: 75 SD rats were randomized evenly into 5 groups: control group

non acupoint group

"Zusanli"(ST 36) group

"Sanyinjiao"(SP 6) group and "Zusanli"+ "Sanyinjiao" group. We had measured the pH of fasting gastric juice as well as its volume and acidity. Meanwhile

radioimmunoassay was applied to detect the contents of gastrin (GAS) and epidermal growth factor (EGF) in plasma and gastric juice. EA parameters were frequency of 2 ～15 Hz

voltage of 20～ 35 V (blank load)

dense sparse waves and duration of 30 min. Results: After EA

the volume of fasting gastric juice in both of "Zusanli" group and "Sanyinjiao" group decreased significantly. PH values of the gastric juice of these group had no apparent changes. The acidity of "Zusanli" group and "Zusanli"+ "Sanyinjiao" group lowered significantly (P< 0.05～0.01) . GAS content of gastric juice in "Zusanli" group decreased obviously (P<0.05) while EGF in the gastric juice increased significantly (P<0.01). The contents of both EGF and GAS in gastric juice in "Sanyinjiao" group increased significantly. There was no interrelation between EGF and GAS of gastric juice in the changes. In"Zusanli"group

despite no significant decrease of plasma EGF was found

there still was a positive correlation between plasma EGF and GAS (r=0.6213

P<0.05). In "Zusanli"+ "Sanyinjiao" group

there wasa negative correlation between plasma EGF and GAS decrease (r=-0.72

P<0.01). There was correlation between plasma EGF and GAS was found in "Sanyinjiao" group and non acupoint group. Conclusion: EA of both "Zusanli" and "Sanyinjiao" can suppress gastric acid secretion singly and also has a synergic action. EA of "Zusanli" can function in suppressing release of GAS and accelerating the secretion of gastric EGF

while EA of "Sanyinjiao" may promote the release of both GAS and EGF in the stomach. There exists certain specificity in the effect of different acupoint stimulation on gastrointestinal hormone activity.