目的 :本研究采用电生理学与形态学相结合的方法

探讨了猫心包经“天泉”穴区与心脏联系的途径。方法 :电生理学部分共用健康猫 3 4只

乌拉坦 ( 50 0mg/kg)与氯醛糖 ( 50mg/kg

i.p .)混合麻醉

用双极铂金丝电极和针灸针分别记录“天泉”穴区的皮神经放电和肌电。形态学部分 :共用健康猫 1 0只

1 %戊巴比妥钠 ( 40mg/kg

i.p .)麻醉

在左侧“天泉”穴区皮神经、肌肉和左侧心下神经、星状神经节分别注射PI(PropidiumIodide)和bB(bisBenzimide)。结果 :依次分别切断脊髓T2 ～C7节段的背根腹根后

电刺激猫左心下神经诱发“天泉”穴区肌电和肌皮神经放电反应所需阈值逐渐增大

尤以T1 、C8和C7为甚 ;左侧“天泉”穴区皮神经、肌肉内和心下神经、星状神经节内分别注射微量PI与bB后

单标及双标神经元也以C7、C8和T1 节段背根节内居多

其中C8最多。结论 :心包经与心脏之间的相互联系主要发生在C7～T1节段Objective: To explore the connection pathway between "Tianquan" (PC 2) of the Pericardium Meridian and the heart with the combined electrophysiological and morphological techniques. Methods: 34 cats anesthetized with urethane (500 mg/kg) and chloralose (50 mg/kg) were used in the first part of the present study. ① After isolating the left inferior cardiac nerve (LICN) and the left lateral cutaneous nerve (LLCN) and performing a skin flap bath of paraffin-oil

discharges of LCN were fed to VC-10 oscilloscope and histogramer via a bipolar platinum electrode (reference electrode placed beneath the subcutaneous tissues)

and recorded by using a X-Y recorder. LICN was stimulated with an electric stimulator. The myoelectrical activities of "Tianquan" (PC 2) and "Tianfu" (LU 3) areas were recorded respectively through two pairs of acupuncture needle electrodes. The reaction threshold was set to that the electric stimulation just could evoke myoelectric signal changes. ② In the second part of this study

ten cats anesthetized with 1% pentobarbital (40 mg/kg) were used. Propidium Iodide (PI

10 μL) was carefully injected into the sheath of LLCN and the muscles around "Tianquan" (PC 2) and bis Benzimide (bB

5 μL) injected respectively into and the LICN and the stellate ganglion. Results: Following electric stimulation of LICN

the firing rates of LLCN and myoelectrical activity of"Tianquan" (PC 2) increased pronouncedly in comparison with pre-stimulation(P<0.01); while no significant changes of myoelectric signals of "Tianfu" (LU 3) were found. After cutting off the dorsal and ventral roots of C 7～T 2 separately

the thresholds of the discharges of the LLCN and "Tianquan" (PC 2) area responding to stimulation of the LICN increased gradually

being significant in T 1

C 8 and C 7 in comparison with pre-section of nerves. After microinjection of PI and bB

light microscopic examination showed that the single and the double labeled neurons were mainly located in C 7

C 8 and T 1 dorsal root ganglions with C 8 being the utmost. Conclusion: a) There exists a relatively specific connection between "Tianquan" (PC 2) and the heart; b) C 7～T 1 segments may be responsible for the connection between "Tianquan" (PC 2) of the Pericardium Meridian and the heart.