目的 :本实验采用小鼠强迫游泳实验的方法

观察针刺合用抗抑郁药后小鼠在水中的静止时间

以此来分析抑郁程度的改善情况。方法 :实验分下列各组 :①生理盐水组 ;②电针加生理盐水组 ;③药物组

分别观察了氯丙咪嗪 (氯米帕明 2 .5mg/kg、5mg/kg、1 0mg/kg、2 0mg/kg、60mg/kg)、芬氟明 ( 1 .5mg/kg、3mg/kg、1 2mg/kg)和吗氯贝胺 ( 1 0mg/kg、2 0mg/kg、40mg/kg)等三种药物的作用 ;④上述药物加电针组。结果 :电针能使小鼠在水中静止的时间显著减少

腹腔注射 (ip)上述三种药物也能减少小鼠在水中静止的时间

并表现出一定的剂量依赖关系。在电针加药组中

分别选用三种药物的小剂量 (抗抑郁效果很弱或几乎无效 )与电针合用。氯丙咪嗪( 2 .5mg/kg)、芬氟明 ( 1 .5mg/kg)与电针合用后

小鼠在水中静止时间与单用药物组或单电针组相比显著减少。而吗氯贝胺 ( 1 0mg/kg)与电针合用虽也有这种趋势

但统计学上尚未达到显著性差异。结论 :电针具有抗抑郁作用

电针合用某些小剂量抗抑郁药后即能进一步加强这种效应Objective: In the present study

the effect of electroacupuncture(EA) combined with drugs on the depression was observed by a forced swimming test. Methods: Male Kunming mice were randomly divided into: ① normal saline(NS

n=10) group

② NS plus EA group (n=10)

③ drug groups--chlorimipramine(2.5 mg/kg

5 mg/kg

10 mg/kg

20 mg/kg

60 mg/kg)

fenfluramine(1.5 mg/kg

3 mg/kg

12 mg/kg) and moclobemide(10 mg/kg

20 mg/kg

40 mg/kg)

④ drug plus EA groups (chlorimipramine 2.5 mg/kg+EA

fenfluramine 1.5 mg/kg + EA

and moclobemide 10 mg/kg + EA). The number of each one-dosage subgroup of each drug in drug groups and drug plus EA groups was 10. EA (sparse waves 4 Hz

series duration 2.5 sec

dense waves 20 Hz

series duration 5 sec

strength≤1 mA) was applied to "Baihui"(GV 20) and Anmian (EX-HN 16) for 40 min. These 3 types of drugs were given by intraperitoneal injection. Results: In comparison with NS group

the duration of immobility (DI) during 5 min test in EA plus NS group was decreased significantly (P<0.01). Compared with that of NS group

the values of DI in chlorimipramine (2.5～ 60 mg/kg

slight dose-dependent tendency)

fenfluramine (3～6 mg/kg

no dose-dependent tendency) and moclobemide (10～40 mg/kg

marked dose-effect dependent relationship

r=0.998) groups were also reduced pronouncedly (P<0.01). When the low dose of chlorimipramine (2.5 mg/kg) and fenfluramine (1.5 mg/kg)

which hardly had antidepressant effect

were combined with EA

DI values were decreased significantly more than those of either EA plus NS group or drug groups (P< 0.01). The DI of Moclobemide(10 mg/kg) plus EA was smaller remarkably than that of Moclobemide (10 mg/kg

P<0.01)

but had no statistical difference compared with that of EA+NS group (P>0.05). Conclusion: EA has an antidepressant effect and EA plus some antidepressants in low doses can potentiate the action of EA further

suggesting that EA plus antidepressant may be a good way for treating depression.