随着人口老龄化

心脑血管疾患日益增多

心脑等脏器疾病是造成临床致死、致残的主要原因

目前仍采用综合防治方法。针刺对心脑缺血再灌注有防治作用

但其作用机制还不完全清楚。本文汇总了近几年从基因水平研究针刺对缺血再灌注的作用的文献

主要描述了针刺对缺血再灌注后心脑组织细胞的基因变化的影响

包括热休克蛋白、原癌基因、细胞因子、Bcl 2等

并描述了作用机制

从而概括地说明针刺在缺血再灌注后损伤中发挥的作用In the present paper

the authors review recent advances of researches on the effect of acupuncture on cellular gene expression in ischemia-reperfusion (IR) heart and brain tissues. Acupuncture has a good protective effect on the IR injured tissues of the heart and brain

but the underlying mechanisms have not been clarified completely up to now. This paper gathers some literature and tries to elucidate the favorable effect of acupuncture treatment from following aspects: 1) Effect of acupuncture on heat shock protein: Heat shock protein (HSP) family including HSP90

HSP70

HSP72 and HSP60 may increase in gene expression in response to environmental stimulation

simultaneously

the cells increase their endurance upon new stimulation. Acupuncture stimulation can up-regulate expression of myocardial HSP mRNA and neurons of the cerebral ischemic penumbra. 2) Effect of acupuncture on c-fos expression in IR brain tissue: In the early period of cerebral ischemia

increase of c-fos mRNA may be helpful to the recovery and re-encoding of protein transcription. After acupuncture stimulation

c-fos mRNA expression in some ischemic cerebral regions is increased further; on the other hand

acupuncture can also suppress the over-expression of c-fos

which may contribute to the protective action of acupuncture on ischemic neurons. 3) Effect of acupuncture on cerebral (superoxide dismutase) SOD: Acupuncture can raise SOD activity of the ischemic cerebral tissues to resist free radicals-induced injury. 4) Effect of acupuncture on cytokines: Acupuncture stimulation can raise IL-2 level. Cerebral IR may induce expression of IL-1Ra mRNA of the cerebral cortex

following acupuncture

its expression increases further. 5) Effect of acupuncture on Bcl-2 expression: EA may up-regulate expression of Bcl-2 in the IR cerebral tissue to resist apoptosis. 6) In addition

acupuncture may also promote expression of nerve growth factor (NGF).