Objective: To explore the morphological connective pathways between the cardiac Shu and Mu acupoints and the heart. Methods: Thirty Wistar rats anesthetized with pentobarbital (40 mg/kg) were randomly and equally divided into "Xinshu" (BL 15)

"Juque" (CV 14) and "Xinshu" "Juque" groups. 5% Propidium Iodide (PI

15 μL) was separately injected into the acupoint areas and 10% Bisbenzimide (Bb

20 μL) was injected into the heart following thoractomy under anesthesia in each group. After surviving for 12 hours

the rats were anesthetized with pentobarbital again and infused with saline and 4% paraformaldehyde phosphate buffer solution respectively

followed by taking out the spinal dorsal root ganglions to be cut into sections (40 μm thick). Under microscope

the labeled neurons were determined.Results: Results indicated that

the mono labeled neurons of "Xinshu" (BL 15) and heart were located in the ganglions of T 1～T 6 and T 2～T 5 respectively both with T 5 being predominant. The doubly labeled neurons were located in the ganglions of T 2～T 5

with T 5 being predominant. The mono labeled neurons from "Juque" (CV 14) were located in T 1～T 7 ganglions and the doubly labeled neurons in T 2～T 5 ganglions

also with T 5 being predominant. Conclusion: T 2～T 5 ganglions

predominantly T 5 ganglions

are one of the morphological base for the specific connection between cardiac Shu and Mu acupoints and the heart.