Objective: To explore the effect of accelerated aging and dementia on cerebral proteome and the regulative effect of acupuncture interference.Methods: Forty eight-month-old senescence-accelerated mice prone10(SAMP10) and their homologue

normal aging mice(SAMR1) of the same age were randomly and evenly assigned to P10-acupuncture

P10-sham-acupuncture

P10-control and R1-control groups with 10 cases in each group.P10-acupuncture group was treated by acupuncture of "Shanzhong"(CV 17)

"Zhongwan"(CV 12)

"Qihai"(CV 6)

"Xuehai"(SP 10) and "Zusanli"(ST 36)

once daily and continuously for 15 days except the 7

(th)

day.The sham-acupuncture group was treated by acupuncture of non-acupoint at the hypochondrium.The acupuncture needle was twirled rapidly with reinforcing method about(30 s) every time for each acupoint.At the end of the experiments

the mice under deep anesthesia were killed for sampling the brain tissue.After a series of process of the brain tissue

the expressive profiles of cerebral proteins were displayed by applying bi-dimensional electrophoresis technique.Results: With the accelerated senescence and dementia

the expression of 1 protein spot((100 kD/pI 5.5)) appeared and 2 proteins disappeared simultaneously in SAMP10 of P10-acupuncture

P10-sham-acupuncture and P10-control groups

but appeared in SAMR1.In addition

in SAMP10 group

6 proteins displayed abnormal expression including 2 proteins being down-regulated and 4 proteins up-regulated in the expression level which were reversed in P10-acupuncture group

suggesting acupuncture may alleviate the effect of aging and dementia.In P10-sham-acupuncture group

of the 4 proteins being up-regulated in the expression in SAMP10 group

2 proteins showed up-regulation and the other 2 down-regulation.Conclusion: Some proteins of the cerebrum in SAMP10 show aberrant expression with the accelerated senescence

which can be improved by acupuncture.