Objective:To probe into the relationship between “Xinshu”(BL 15)-“Jueyinshu”(BL 14)of the Bladder Channel and the heart and the underlying mechanism of electroacupuncture(EA)in resisting acute myocardial ischemia(AMI)in rats.Methods:A total of 24 male Wistar rats were randomly divided into control

model and EA groups with 8 cases in each group.AMI model was duplicated by intraperitoneal injection of 2% isoproterenol(ISO

100 mg/kg).EA(pulse duration 300 μs

frequency 2-20 Hz

output voltage 3.5-5 V)was applied to BL14 and BL15 on the left side for 30 min

twice in a day(with an interval of 12 h).After decapitation

blood samples

myocardial tissue and spinal cord(T1-T6)were collected respectively for detecting serum creatine kinase(CK

with chromatometry)and calcitonin gene-related peptide(CGRP)contents in the plasma

myocardium and the spinal cord(with radioimmunoassay).Results:In comparison with control group

serum CK of both model group and EA group increased significantly(P<0.01)

and plasma CGRP content of model group lowered lightly;while compared with model group

CK content of EA group decreased obviously(P<0.05)and plasma CGRP of EA group increased evidently(P<0.05).No significant differences were found among 3 groups in myocardial CGRP contents and between control group and model group in spinal CGRP contents(P>0.05).Conclusion:EA of “Xinshu”(BL 15)-“Jueyinshu”(BL 14)may suppress isoproterenol-injection induced myocardial injury and plasma CGRP possibly takes part in the protective effect of EA in resisting myocardial ischemia injury.