Selection of Basic Acupoints for Composing "Gastric-Disorder-Formula" for Electroacupuncture Prevention of Acute Gastric Mucosal Lesion in Rats
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Selection of Basic Acupoints for Composing "Gastric-Disorder-Formula" for Electroacupuncture Prevention of Acute Gastric Mucosal Lesion in Rats
Acupuncture ResearchIssue 5, Pages: 296-300(2008)
作者机构:
1. 山西中医学院针灸系
2. 湖北中医学院针灸系
3. 山西省针灸研究所实验中心
作者简介:
基金信息:
DOI:
CLC:
Published:2008
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JI Lai-xi, YAN Li-ping, WANG Hai-jun, et al. Selection of Basic Acupoints for Composing "Gastric-Disorder-Formula" for Electroacupuncture Prevention of Acute Gastric Mucosal Lesion in Rats[J]. Acupuncture research, 2008, (5): 296-300.
DOI:
JI Lai-xi, YAN Li-ping, WANG Hai-jun, et al. Selection of Basic Acupoints for Composing "Gastric-Disorder-Formula" for Electroacupuncture Prevention of Acute Gastric Mucosal Lesion in Rats[J]. Acupuncture research, 2008, (5): 296-300.DOI:
Selection of Basic Acupoints for Composing "Gastric-Disorder-Formula" for Electroacupuncture Prevention of Acute Gastric Mucosal Lesion in Rats
Objective To select the best combination of the commonly-used acupoints [Zhongwan(CV 12)
Neiguan(PC 6)
and Zusanli(ST 36)] for treatment of gastric disorders.Methods Forty-eight Wistar rats were randomly divided into 8 groups: model
ST36
CV12
PC6
ST36+PC6
ST36+CV12
CV12+PC6 and ST36+CV12+PC6 by factorial design method.Acute gastric mucosal lesion model was established by intragastric perfusion of anhydrous alcohol.EA(1-3 mA
30 Hz)was applied to the above-mentioned acupoints for 20 min respectively.And after EA
gastric mucosal lesion index(LI)
pathological lesion integral score(PLIS) were calculated and mucosal ultramicrostructural changes observed by optical microscope and transmission electron microscope respectively.Results Compared with model group
the gastric mucosal LI and PLIS of ST36
CV12
PC6
ST36+CV12
ST36+PC6
CV12+PC6
ST36+CV12+PC6 groups all decreased significantly(P<0.05).Comparison among 7 EA groups showed that both LI and PLIS of ST36+CV12+PC6 group were significantly lower than those of ST36
CV12
PC6
ST36+CV12
ST36+PC6 and CV12+PC6 groups(P<0.05).No significant differences were found between every two groups of ST36
CV12
PC6
ST36+CV12
ST36+PC6 and CV12+PC6 groups(P>0.05) in both LI and PLIS.Ultrastructural results showed that the injury of gastric mucosal parietal cells and delomorphous cells including mitochondrial swelling
crista arrange disorder and breaking
enlargement of the rough endoplasmic reticulum
ribosome exfoliation
etc.in model group was serious
but lightened significantly in EA groups particularly in ST36+CV12+PC6 group.Conclusion ST36
PC6 and CV12 combination can effectively reduce anhydrous alcohol-induced gastric mucosal lesion in rats
and may be used as a basic formula for treatment of stomach diseases.
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