Differentially Expressed Genes Involving the Correlativity Between Heart Meridian and Hypothalamus
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Differentially Expressed Genes Involving the Correlativity Between Heart Meridian and Hypothalamus
Acupuncture ResearchIssue 4, Pages: 219-222(2008)
作者机构:
安徽中医学院经脉脏腑相关研究中心
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Published:2008
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LI Meng, LONG Di-he, HE Lu, et al. Differentially Expressed Genes Involving the Correlativity Between Heart Meridian and Hypothalamus[J]. Acupuncture research, 2008, (4): 219-222.
DOI:
LI Meng, LONG Di-he, HE Lu, et al. Differentially Expressed Genes Involving the Correlativity Between Heart Meridian and Hypothalamus[J]. Acupuncture research, 2008, (4): 219-222.DOI:
Differentially Expressed Genes Involving the Correlativity Between Heart Meridian and Hypothalamus
Objective To study the bio-molecule basis of correlativity between Heart Meridian and hypothalamus from gene differential expression. Methods Twenty-eight healthy Sprague-Dawley male rats were divided into 4 groups randomly: normal control
model
Heart Meridian-electroacupuncture (EA) and Lung Meridian-EA groups
with 7 cases in each. Acute myocardial ischemia (AMI) model was duplicated by occlusion of the left anterior descending branch (LAD) of the coronary artery under anesthesia (3% Nemb
40 mg/kg). EA (1.1 mA
2 Hz
duration of waves 300 μs) was applied to bilateral "Shenmen" (HT 7)-"Tongli" (HT 5
Heart Meridian)
"Taiyuan" (LU 9)-"Lieque" (LU 7
Lung Meridian) separately for 20 min
once daily for 3 days. Then
hypothalamus tissue was taken for differentially expressed gene profiling analysis (Affymetrix GeneChip Rat Genome U 230 Affymetrix). Results Compared with model group
among the differentially expressed genes above 2 times in Heart-Meridian-EA group and Lung-Meridian-EA group
190 and 57 were up-regulated
34 and 26 down-regulated separately. Compared with Lung-Meridian-EA group
among the 175 differentially expressed genes above 2 times in hypothalamus tissues of Heart-Meridian-EA group
147 were up-regulated and 28 down-regulated. Conclusion In comparison with EA of Lung Meridian
a different gene expression profile of hypothalamus tissue is involved in the effect of EA of Heart-Meridian on acute myocardial ischemia.
Effect of Electroacupuncture at “Shenmen”(HT 7)-“Tongli”(HT 5) of Heart Meridian on Neuronal Activities in Paraventricular Nucleus of Hypothalamus in Myocardial Ischemia Rats
Effect of Electroacupuncture Stimulation of Heart and Lung Meridians on Expression of Myocardial HCN 2 in Acute Myocardial Ischemia Rats
Correlation between acupoint sensitization of body surface and pituitary adenylate cyclase activating polypeptide expression in myocardial ischemia mice
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WANG Jie
PENG Chuan-yu
HU Ling
Related Institution
Research Institute of Acupuncture-moxibustion and Meridians,Anhui University of Traditional Chinese Medicine
Section of Science and Technology,Anhui University of Traditional Chinese Medicine
Institute of Acupuncture and Meridian, Anhui Academy of Traditional Chinese Medicine
Clinical College of Acupuncture-moxibustion and Orthopedics-traumatology,Anhui University of Traditional Chinese Medicine
Graduate School,Anhui University of Traditional Chinese Medicine