Observation on Changes of the Intragastric Protein Phosphorylation Level Involving Electroacupuncture-induced Improvement of the Injured Gastric Mucosa in the Rat

TIAN Hao-mei; YAN Jie; YI Shou-xiang; ZHANG Ying-jin; YAO Wen

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Observation on Changes of the Intragastric Protein Phosphorylation Level Involving Electroacupuncture-induced Improvement of the Injured Gastric Mucosa in the Rat

更新时间：2023-08-11

- Observation on Changes of the Intragastric Protein Phosphorylation Level Involving Electroacupuncture-induced Improvement of the Injured Gastric Mucosa in the Rat
- Acupuncture Research Vol. 34, Issue 3, Pages: 147-151(2009)
- 作者机构：
  
  湖南中医药大学针灸推拿学院
- 作者简介：
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- Published：2009
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TIAN Hao-mei, YAN Jie, YI Shou-xiang, et al. Observation on Changes of the Intragastric Protein Phosphorylation Level Involving Electroacupuncture-induced Improvement of the Injured Gastric Mucosa in the Rat[J]. Acupuncture research, 2009, 34(3): 147-151.
DOI：

TIAN Hao-mei, YAN Jie, YI Shou-xiang, et al. Observation on Changes of the Intragastric Protein Phosphorylation Level Involving Electroacupuncture-induced Improvement of the Injured Gastric Mucosa in the Rat[J]. Acupuncture research, 2009, 34(3): 147-151. DOI：

摘要

目的:探讨电针促胃黏膜修复过程中对相关蛋白磷酸化的影响。方法:20只SD大鼠分为空白组、模型组、针刺非穴组和针刺穴位组

每组5只。使用无水乙醇灌胃法制作大鼠胃黏膜损伤模型。针刺非穴组取"足三里""梁门""四白"穴旁的对照点

针刺穴位组取"足三里""梁门""四白"穴

每次电针30 min

每日1次

共治疗7 d。对大鼠的胃黏膜细胞分别进行磷酸化抗体蛋白芯片检测

同时筛选720种磷酸化蛋白的表达差异

并进行比较。结果:模型组胃黏膜损伤指数与空白组比较显著升高(P<0.01);针刺穴位组胃黏膜损伤指数低于模型组和针刺非穴组(P<0.01)

而与空白组比较差异无统计学意义(P>0.05)。蛋白芯片分析示:针刺穴位组与针刺非穴组均可上调模型大鼠的蛋白磷酸化的水平(≥1.5倍)

但针刺穴位组上调的蛋白大多数参与了细胞的增殖

而针刺非穴组只有几种蛋白参与细胞增殖;同时针刺穴位组与针刺非穴组还可下调蛋白磷酸化水平(≥1.5倍)

且针刺组下调的蛋白参与抑制细胞凋亡

而针刺非穴组下调的蛋白基本不参与细胞活动。结论:电针足阳明胃经穴可引起大鼠胃黏膜损伤后修复信号蛋白的磷酸化水平发生变化

提示电针促进胃黏膜修复的机制与胃黏膜损伤后修复信号蛋白的磷酸化有关。

Abstract

Objective To study the effect of electroacupuncture(EA) of Stomach Meridian acupoints on changes of the related protein phosphorylation in the gastric mucosa involving the repair of the injured gastric mucosa in the rat.Methods A total of 20 SD rats were divided into control

model

acupoint and non-acupoint groups

with 5 cases in each.Gastric mucosa injury model was established by intragastric perfusion of dehydrated alcohol(0.8 ml/100 g).EA(4 Hz/50 Hz

1-3 mA) was applied to "Zusanli"(ST 36)

"Liangmen"(ST 21) and "Sibai"(ST 2)

and the corresponding non-acupoints

once daily for 7 days.Gastric mucosal ulcer index(UI) was measured

and the profiling of the protein phosphorylation of interest in gastric mucosa cells was detected by antibody microarrays.Results In comparison with control group

the gastric mucosal UI of model group was significantly higher than that of control group(P<0.01)

while the UI of EA group was significant lower than those of model group and non-acupoint group(P<0.01)

suggesting a recovery of the injured mucous membrane.Findings of protein array analysis indicated that a total of 720 kinds of phosphorylated proteins(≥1.5 folds)were found in the injured gastric mucosa.Compared to model group

the phosphorylation levels of 100 and 20 proteins were up-regulated

and 16 and 9 proteins down-regulated in acupoint and non-acupoint groups

respectively.The up-regulated proteins involve cell cycle mediation

cell proliferation

anti-apoptosis

and the regulation of the cell adhesion

cytoskeleton protein

inflammation

immune activities

etc.while the down-regulated proteins involve the down-regulation of the phosphorylation levels of the apoptosis proteins

adhesion proteins and cytoskeleton proteins

etc.In comparison to non-acupoint group

the phosphorylation levels of 100 proteins were up-regulated and 16 proteins down-regulated in acupoint group.Conclusion EA of acupoints of the Stomach Meridian can promote repair of the injured gastric mucosa

which may be related to its effects in regulating the levels of phosphorylation of many signaling proteins.

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