Effect of Electroacupuncture at "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) on Collagen-induced Arthritis and Secretory Function of Knee-joint Synoviocytes in Rats

FANG Jian-qiao; SHAO Xiao-mei; MA Gui-zhi

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Effect of Electroacupuncture at "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) on Collagen-induced Arthritis and Secretory Function of Knee-joint Synoviocytes in Rats

更新时间：2023-08-11

- Effect of Electroacupuncture at "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) on Collagen-induced Arthritis and Secretory Function of Knee-joint Synoviocytes in Rats
- Acupuncture Research Vol. 34, Issue 2, Pages: 93-96(2009)
- 作者机构：
  
  浙江中医药大学第三临床医学院
- 作者简介：
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- Published：2009
- 稿件说明：
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FANG Jian-qiao, SHAO Xiao-mei, MA Gui-zhi. Effect of Electroacupuncture at "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) on Collagen-induced Arthritis and Secretory Function of Knee-joint Synoviocytes in Rats[J]. Acupuncture research, 2009, 34(2): 93-96.
DOI：

FANG Jian-qiao, SHAO Xiao-mei, MA Gui-zhi. Effect of Electroacupuncture at "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) on Collagen-induced Arthritis and Secretory Function of Knee-joint Synoviocytes in Rats[J]. Acupuncture research, 2009, 34(2): 93-96. DOI：

摘要

目的:观察电针"足三里""三阴交"对胶原性关节炎(collagen-induced arthritis

CIA)大鼠的治疗作用

并探讨该疗法调节CIA大鼠滑膜细胞分泌功能的部分机制。方法:36只Wistar大鼠随机分为正常对照组、模型对照组和电针治疗组。模型对照组和电针治疗组采用牛Ⅱ型胶原在大鼠背部正中线作多点皮内注射诱发CIA模型。电针治疗组选取"足三里""三阴交"穴进行电针刺激

频率2Hz

强度2mA

时间30min

连续治疗30d。处理前后分别观察CIA大鼠足跖肿胀度、痛阈;采用ELISA法检测大鼠膝关节滑膜细胞培养上清液中前列腺素E2(PGE2)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)水平。结果:模型对照组大鼠足跖肿胀度

滑膜细胞培养上清中PGE2浓度和TNF-α、IL-1β含量均高于正常对照组大鼠

差异有统计学意义(P<0.05

P<0.01)

痛阈低于正常对照组大鼠

差异有统计学意义(P<0.05)。与模型对照组比较

电针治疗组大鼠足跖肿胀度减小

痛阈升高

关节滑膜细胞培养上清中PGE2浓度下降

TNF-α和IL-1β含量降低

差异有统计学意义(P<0.05)。结论:电针"足三里""三阴交"穴对胶原性关节炎大鼠具有良好的治疗作用

该作用可能与抑制关节滑膜细胞的分泌功能有关。

Abstract

Objective To observe the effect of electroacupuncture(EA)of "Zusanli"(ST 36)and "Sanyinjiao"(SP 6)on collagen-induced arthritis(CIA)and its underlying mechanism in regulating the secretory function of knee-joint synovial cells in CIA rats.Methods Thirty-six Wistar rats were equally randomized into control

model and EA groups.CIA model(rheumatoid arthritis)was duplicated by intradermal injection of Bovine type Ⅱ collagen into the back of the anesthetized rats.EA(2 Hz

2 mA)was applied to "Zusanli"(ST 36)and "Sanyinjiao"(SP 6)for 30 min

once a day for 30 days.The paw volume was measured by using a Plethysmometer and hot water tail-flick tests(50 ℃)were conducted for detecting the rats' pain threshold(PT)before and after the treatment.The contents of PGE2

IL-1β and TNF-α in supernatant of the cultured joint synoviocytes were detected by enzyme linked immunosorbent assay(ELISA).Results In comparison with control group

the swelled paw volume

the declined PT

PGE2

TNF-α and IL-1β contents in knee-joint synoviocyte supernatant were significantly higher in model group(P<0.05).Compared with model group

PT increased obviously(P<0.05);paw volume

supernatant PGE2

TNF-α and IL-1β contents decreased obviously(P<0.05)in EA group.Conclusion EA of ST 36 and SP 6 has a pronounced therapeutic effect in relieving knee joint pain and inflammation in CIA rats

which may be related to its effect in regulating the secretory function of the knee-joint synoviocytes.

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