Effect of Electroacupuncture Stimulation of Heart and Lung Meridians on Expression of Myocardial HCN 2 in Acute Myocardial Ischemia Rats

您当前的位置：

首页 >

文章列表页 >

Effect of Electroacupuncture Stimulation of Heart and Lung Meridians on Expression of Myocardial HCN 2 in Acute Myocardial Ischemia Rats

更新时间：2023-08-11

- Effect of Electroacupuncture Stimulation of Heart and Lung Meridians on Expression of Myocardial HCN 2 in Acute Myocardial Ischemia Rats
- Acupuncture Research Vol. 43, Issue 3, Pages: 175-179(2018)
- 作者机构：
  
  1. 安徽中医药大学科技处
  2. 安徽省中医药科学院针灸经络研究所
  3. 安徽中医药大学针灸骨伤临床学院
  4. 安徽中医药大学研究生院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.170121
  CLC：
- Published：2018
- 稿件说明：
移动端阅览
WU Zi-jian, WANG Jie, DUAN Wen-xiu, et al. Effect of Electroacupuncture Stimulation of Heart and Lung Meridians on Expression of Myocardial HCN 2 in Acute Myocardial Ischemia Rats[J]. Acupuncture research, 2018, 43(3): 175-179.
DOI：

WU Zi-jian, WANG Jie, DUAN Wen-xiu, et al. Effect of Electroacupuncture Stimulation of Heart and Lung Meridians on Expression of Myocardial HCN 2 in Acute Myocardial Ischemia Rats[J]. Acupuncture research, 2018, 43(3): 175-179. DOI： 10.13702/j.1000-0607.170121.

摘要

目的:通过比较电针心经和肺经腧穴对急性心肌缺血(AMI)大鼠心肌组织超极化激活的环核苷酸门控通道2(HCN 2)mRNA和蛋白表达的影响

探讨电针经穴效应及后效应的相对特异性。方法:SD大鼠随机分为正常组、模型组、电针手少阴心经神门组(简称神门组)、电针手太阴肺经太渊组(简称太渊组)

每组12只。采用冠状动脉前降支结扎法复制AMI模型。两电针组分别电针"神门""太渊"穴

刺激15min

1次/d

共治疗7d。于末次电针治疗后即刻和次日相同时间各取材6只大鼠

分别称为神门8d组、神门9d组、太渊8d组和太渊9d组。采用实时荧光定量PCR法检测心肌组织HCN 2mRNA表达水平

Western blot法检测HCN 2蛋白表达水平。结果:与正常组比较

模型组HCN 2mRNA和蛋白表达水平均显著减少(P<0.01);与模型组比较

神门组及太渊组HCN 2mRNA和蛋白表达量均显著增多(P<0.01);与神门8d组比较

神门9d组的HCN 2mRNA和蛋白表达量均显著增多(P<0.01)

但太渊8d组的HCN 2mRNA和蛋白表达量均减少(P<0.01);与神门9d组比较

太渊9d组的HCN 2mRNA和蛋白表达量均显著减少(P<0.01);与太渊8d组比较

太渊9d组HCN 2蛋白表达显著增加(P<0.01)。结论:电针心经和肺经均可以促进AMI模型大鼠心肌组织HCN 2mRNA和蛋白的表达

且具有一定的持续性后效应

电针心经在调整心肌组织HCN 2表达中具有相对特异性。

Abstract

Objective To observe the effect of electroacupuncture(EA)stimulation of Heart and Lung meridians on the expression of hyperpolarization-activated cyclic nucleotide gated potassium channel 2(HCN 2)mRNA and protein in the myocardium of the left ventricle in acute myocardial ischemia(AMI)rats

so as to underlying its mechanism in improving ischemic myocardial injury.Methods A total of 120 male SD rats were randomly divided into normal control

model

EA-Heart Meridian(HT)and EA-Lung Meridian(LU)groups(n=30 rats in each group).The AMI model was established by occlusion of the anterior descending branch of coronary artery.EA(1 mA

10 Hz)was applied to bilateral"Shenmen"(HT 7)or"Taiyuan"(LU 9)and the spot about 1 mm apart from the HT 7 or LU 9 on the axopetal end for 15 min on the second day after modeling

once a day for 7 days.The left ventricular myocardium tissue was sampled for determining the expression levels of HCN 2 mRNA and protein with qPCR and Western blot

separately.Results After AMI

both HCN 2 mRNA and protein expression levels in the model group were significantly decreased in comparison with the normal control group(P<0.01).Following EA intervention

the expression levels of HCN 2 mRNA and protein expressions were significantly up-regulated relevant to the model group(P<0.01).The effects were more obvious in the EA-HT group than in the EA-LU group

and the expression levels were higher in the left ventricular myocardium tissue sampled on the next day after the treatment than those in the tissue sampled immediately after the treatment(P<0.01).Conclusion Both EA stimulation of Heart Meridian and Lung Meridian can improve the expression of HCN 2 mRNA and protein in the myocardium in AMI rats

which existing a delayed effect and meridian specificity.

关键词

Keywords

references

0

Views

350

下载量

13

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Related Articles

Effect of Electroacupuncture at “Shenmen”(HT 7)-“Tongli”(HT 5) of Heart Meridian on Neuronal Activities in Paraventricular Nucleus of Hypothalamus in Myocardial Ischemia Rats

Electroacupuncture improves ischemic myocardial injury by activating Nrf2/HO-1 signaling pathway to inhibit ferroptosis in rats

Electroacupuncture ameliorates inflammatory response of mice with acute myocardial ischemia by regulating vascular endothelial growth factor C/vascular endothelial growth factor receptor 3 pathway

Related Author

CAI Rong-lin

CUI Shuai

WU Zi-jian

HE Lu

YU Qing

WANG Jie

PENG Chuan-yu

HU Ling

Related Institution

Research Institute of Acupuncture-moxibustion and Meridians,Anhui University of Traditional Chinese Medicine

Center for Researching Meridian-Viscera Correlation, Anhui College of Chinese Medicine

Center for Researching Correlation Between Meridians and Zangfu-organs,Anhui College of Chinese Medicine

Anhui Wanbei Coal-electricity Group General Hospital of Rehabilitation

College of Acupuncture-moxibustion and Tuina, Anhui University of Chinese Medicine

Postal code：100700
Tel：010-64089344,84014607 Email：zcyjbjb@vip.163.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05017590号-8 京公网安备11010802020460号
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰