Effect of Manual Acupuncture Preconditioning on Behavior and Contents of Serum CGRP,SP,IL-1β and TNF-α Levels in Migraine Rats

WANG Meng-meng; YU Xiao-hua; GENG Wei; CUI Hua-feng; WANG Chang-chun; HAN Jing; YANG Dian-hui

doi:10.13702/j.1000-0607.170415

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Effect of Manual Acupuncture Preconditioning on Behavior and Contents of Serum CGRP,SP,IL-1β and TNF-α Levels in Migraine Rats

更新时间：2023-08-11

- Effect of Manual Acupuncture Preconditioning on Behavior and Contents of Serum CGRP,SP,IL-1β and TNF-α Levels in Migraine Rats
- Acupuncture Research Vol. 43, Issue 6, Pages: 375-379(2018)
- 作者机构：
  
  1. 山东中医药大学针灸推拿学院
  2. 山东中医药大学附属医院针灸科
  3. 山东省妇幼保健院中医科
  4. 山东省中医药重点科研实验室针灸神经生物学实验室
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.170415
  CLC：
- Published：2018
- 稿件说明：
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WANG Meng-meng, YU Xiao-hua, GENG Wei, et al. Effect of Manual Acupuncture Preconditioning on Behavior and Contents of Serum CGRP,SP,IL-1β and TNF-α Levels in Migraine Rats[J]. Acupuncture research, 2018, 43(6): 375-379.
DOI：

WANG Meng-meng, YU Xiao-hua, GENG Wei, et al. Effect of Manual Acupuncture Preconditioning on Behavior and Contents of Serum CGRP,SP,IL-1β and TNF-α Levels in Migraine Rats[J]. Acupuncture research, 2018, 43(6): 375-379. DOI： 10.13702/j.1000-0607.170415.

摘要

目的:观察疏肝调神针法对偏头痛大鼠的行为学及血清降钙素基因相关肽(CGRP)、P物质(SP)、白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)表达的影响

探讨疏肝调神针法治疗偏头痛可能的作用机制。方法:Wistar雄性大鼠随机分为对照组、模型组、疏肝调神组、普通针刺组

每组8只。疏肝调神组针刺"百会"及双侧"风池""内关""太冲"

普通针刺组针刺"百会"及双侧"风池"

30min/次

1次/d

连续8d。第8天针刺后采用大鼠颈后皮下注射硝酸甘油制备偏头痛模型。观察造模前后3次行为学评分变化

酶联免疫法检测各组血清中CGRP、SP、IL-1β、TNF-α的浓度。结果:造模后

各组行为学评分均较对照组明显升高(P

0.05);造模后2个评分时间段(60

0min及12090min及120

50min)

与模型组比较

疏肝调神组、普通针刺组行为学评分明显降低(P

0.05);120150min)

与模型组比较

疏肝调神组、普通针刺组行为学评分明显降低(P

0.05);120

50min时

疏肝调神组与普通针刺组评分较60150min时

疏肝调神组与普通针刺组评分较60

0min时明显降低(P

0.05)。与对照组比较

模型组大鼠血清中CGRP、SP、IL-1β、TNF-α的浓度明显升高(P

0.05);与模型组比较

疏肝调神组、普通针刺组大鼠血清中CGRP、SP、IL-1β、TNF-α的浓度明显降低(P

0.05);与普通针刺组比较

疏肝调神组大鼠血清中CGRP、SP、IL-1β、TNF-α的浓度显著降低(P

0.05)。结论:针刺可有效防治偏头痛的发生

疏肝调神针法在抑制偏头痛大鼠血清CGRP、SP及IL-1β、TNF-α等因子的痛觉传导、减轻神经炎性反应中更具优势。

Abstract

Objective To observe the effect of liver-soothing and mental-activity-regulating(LSMAR)needling on behavior reactions and contents of serum calcitonin gene-related peptide(CGRP)

substance P(SP)

interleukin-1β(IL-1β )and tumor necrosis factor-α(TNF-α)in migraine rats

so as to explore its mechanism underlying relief of migraine.Methods Thirtytwo male Wistar rats were randomly divided into control

model

LSMAR needling and conventional needling groups(n=8 rats in each).The migraine model was established by hypodermic injection of glyceryl trinitrate(5 mg/kg)at the napex.Acupuncture preconditioning was conducted once daily for 8 days before modeling.Acupuncture needles were inserted into"Baihui"(GV 20)

and bilateral"Fengchi"(GB 20)

"Neiguan"(PC 6)and "Taichong"(LR 3)

manipulated for a while and retained for 30 min.The rats' behavioral changes(times of head scratching

tail-biting

cage-crawling

and to-and fro-movement in 30 min)were scored(one symptom = one point)before modeling

after modeling

and after intervention.The concentrations of CGRP

IL-1β

TNF-αin serum were assayed by enzyme-linked immunosorbent assay(ELISA).Results After modeling

the behavioral scores were significantly increased in the migraine model rats relevant to the control group(P<0.05).Whereas after the treatment

the behavior scores were considerably decreased during 60-90 min and 120-150 min of the measuring period in both LSMAR and conventional needling groups compared with the model group(P<0.05).The concentrations of CGRP

IL-1β and TNF-αwere significantly higher in the model group than in the control group(P<0.05)

and notably lower in both LSMAR and conventional needling groups than in the model group(P<0.05).The therapeutic effect of LSMAR was markedly superior to that of conventional needling in down-regulating the concentrations of CGRP

IL-1β and TNF-α(P<0.05).Conclusion Acupuncture preconditioning can effectively relieve pain in migraine rats possibly by decreasing the concentrations of CGRP

IL-1β and TNF-αin the peripheral blood

and the therapeutic effect of LSMAR is evidently superior to that of conventional needling.

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