Effect of Electroacupuncture at Different Acupoints on Steroid Hormones and Ovarian Androgen Receptor Expression in Polycystic Ovary Syndrome Rats

XU Ge; WANG Qian; ZHANG An-dong; LIU Jian-dang; FENG Ji-wei; CHEN Yue-lai

doi:10.13702/j.1000-0607.170688

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Effect of Electroacupuncture at Different Acupoints on Steroid Hormones and Ovarian Androgen Receptor Expression in Polycystic Ovary Syndrome Rats

更新时间：2023-08-11

- Effect of Electroacupuncture at Different Acupoints on Steroid Hormones and Ovarian Androgen Receptor Expression in Polycystic Ovary Syndrome Rats
- Acupuncture Research Vol. 43, Issue 9, Pages: 543-549(2018)
- 作者机构：
  
  上海中医药大学岳阳临床医学院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.170688
  CLC：
- Published：2018
- 稿件说明：
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XU Ge, WANG Qian, ZHANG An-dong, et al. Effect of Electroacupuncture at Different Acupoints on Steroid Hormones and Ovarian Androgen Receptor Expression in Polycystic Ovary Syndrome Rats[J]. Acupuncture research, 2018, 43(9): 543-549.
DOI：

XU Ge, WANG Qian, ZHANG An-dong, et al. Effect of Electroacupuncture at Different Acupoints on Steroid Hormones and Ovarian Androgen Receptor Expression in Polycystic Ovary Syndrome Rats[J]. Acupuncture research, 2018, 43(9): 543-549. DOI： 10.13702/j.1000-0607.170688.

摘要

目的:观察电针不同穴位对多囊卵巢综合征(PCOS)大鼠高雄激素血症和卵巢雄激素受体(AR)表达影响的作用差异

探讨电针治疗PCOS的可能机制。方法:SD大鼠随机分为对照组、模型组、足三里组、关元组、三阴交组、综合组

每组10只。采用来曲唑灌胃诱导PCOS模型。足三里组、关元组、三阴交组电针干预相应穴位

综合组同时电针所有穴位

每次20min

每日1次

连续14d。治疗后测量大鼠体质量、卵巢质量

HE染色观察卵巢形态结构

酶联免疫吸附法检测血清性激素水平和性激素结合球蛋白(SHBG)

计算游离雄激素指数(FAI)

免疫组化法检测卵巢卵泡AR的表达。结果:与对照组相比

模型组大鼠体质量和卵巢质量明显增加(P<0.01)

血清睾酮(T)和FAI水平明显升高(P<0.01)

雌二醇(E2)和SHBG水平明显降低(P<0.01)

卵巢晚期卵泡AR蛋白表达增加(P<0.05)。各电针组与模型组比较

体质量均明显减轻(P<0.05)

卵巢形态改善

T和FAI显著降低(P<0.01)

足三里组、关元组和综合组E2、SHBG明显升高(P<0.01

P<0.05)

关元组卵巢质量明显降低(P<0.01)。关元组和三阴交组晚期卵泡AR蛋白表达较模型组降低(P<0.05

P<0.01)。结论:电针不同穴位对PCOS大鼠高雄激素血症和卵巢多囊形态均有改善作用

不同穴位对PCOS大鼠的干预特点不同

临床可根据PCOS患者不同的临床表现选穴。

Abstract

Objective To observe the effect of electroacupuncture(EA)at different acupoints on hyperandrogenism and ovarian androgen receptor(AR)expression in rats with polycystic ovary syndrome(PCOS)

so as to explore its mechanisms underlying improvement of PCOS.Methods Sixty female SD rats were randomly divided into control

model

Zusanli(ST 36)

Sanyinjiao(SP 6)

Guanyuan(CV 4)and comprehensive groups(10 rats/group).The PCOS model was established by giving(gavage)the animals with Letrozole solution(1.0 mg/kg)

once daily for 21 consecutive days.EA stimulation was applied to ST 36

SP 6

or CV 4 respectively

once daily for 14 days.The comprehensive group was treated by EA treatment of the abovementioned three acupoints in the meantime.Before and after the treatment

the body mass and the ovary weight were measured.The morphological structure of the ovary was observed under microscope after H.E.staining.The levels of serum hormone and sex hormone binding globulin(SHBG)were assayed by ELISA.Free androgen index(FAI)was calculated and the expression of AR in the ovary was detected by immunohistochemistry.Results After modeling

the body mass

ovary weight

serum testosterone(T)and FAI levels and the expression of AR protein in late ovarian follicles were significantly increased(P<0.01

P<0.05)

and serum estradiol(E2)and SHBG contents were significantly decreased relevant to the control group(P<0.01).Following EA intervention and compared with the model group

the body weight of each EA group was significantly reduced(P<0.05)

the ovarian pathological changes were improved

and serum T and FAI levels were significantly decreased(P<0.01)

and serum E2 and SHBG contents significantly increased in the ST 36

CV 4 and comprehensive groups(P<0.01

P<0.05).The ovary weight of only CV 4 group was significantly lower than that of the model group(P<0.01).The expression of AR protein in late follicles of both CV 4 and SP 6 groups was markedly decreased(P<0.05

P<0.01).Conclusion EA at ST 36

SP 6

and CV 4 all can improve the hyperandrogenism and ovarian polycystic morphological changes of PCOS rats

while different acupoints have different functional characteristics.

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Related Author

ZHAO Xue-dan

LI Zhi-hao

HU Jun-wei

CHEN Yue-lai

XU Ge

ZHANG An-dong

WANG Xi

Related Institution

Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Teaching and Experimental Centre，Shanghai University of Traditional Chinese Medicine

Teaching and Experimental Center,Shanghai University of Traditional Chinese Medicine

Graduade School, Shanghai University of Traditional Chinese Medicine

Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine

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