Electroacupuncture Improves Limb Locomotor Function Possibly by Suppressing Rho-ROCK Ⅱ Pathway Related Factors in Anterior Horns of Spinal Cord in Rats with Acute Spinal Cord Injury

LI Xiao-ning; LIANG Xue-song; WU Lei; SHAN Xiao-chun; FU Hao; MEI Ji-lin; LI Nuo

doi:10.13702/j.1000-0607.170689

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Electroacupuncture Improves Limb Locomotor Function Possibly by Suppressing Rho-ROCK Ⅱ Pathway Related Factors in Anterior Horns of Spinal Cord in Rats with Acute Spinal Cord Injury

更新时间：2023-08-11

- Electroacupuncture Improves Limb Locomotor Function Possibly by Suppressing Rho-ROCK Ⅱ Pathway Related Factors in Anterior Horns of Spinal Cord in Rats with Acute Spinal Cord Injury
- Acupuncture Research Vol. 43, Issue 7, Pages: 445-449(2018)
- 作者机构：
  
  1. 黑龙江中医药大学附属第二医院针灸四科
  2. 黑龙江中医药大学第二临床医学院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.170689
  CLC：
- Published：2018
- 稿件说明：
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LI Xiao-ning, LIANG Xue-song, WU Lei, et al. Electroacupuncture Improves Limb Locomotor Function Possibly by Suppressing Rho-ROCK Ⅱ Pathway Related Factors in Anterior Horns of Spinal Cord in Rats with Acute Spinal Cord Injury[J]. Acupuncture research, 2018, 43(7): 445-449.
DOI：

LI Xiao-ning, LIANG Xue-song, WU Lei, et al. Electroacupuncture Improves Limb Locomotor Function Possibly by Suppressing Rho-ROCK Ⅱ Pathway Related Factors in Anterior Horns of Spinal Cord in Rats with Acute Spinal Cord Injury[J]. Acupuncture research, 2018, 43(7): 445-449. DOI： 10.13702/j.1000-0607.170689.

摘要

目的:观察夹脊电针对脊髓损伤大鼠神经细胞轴突再生相关抑制因子重组人Ras同源物基因家族成员A(RhoA)、Rho蛋白激酶Ⅱ(ROCKⅡ)、肌球蛋白轻链(MLC)蛋白的影响

探讨夹脊电针治疗急性脊髓损伤(ASCI)的作用机制。方法:雌性Wistar大鼠随机分为假手术组、模型组、夹脊电针组、抑制剂组

每组12只。采用脊髓打击法制备大鼠ASCI模型。夹脊电针组于模型制备成功后3h进行夹脊电针治疗

每日1次

每次30 min

连续治疗14d、28d;抑制剂组于造模成功后立即给予腹腔注射盐酸法舒地尔(10mg/kg)

每日1次

连续治疗14d、28d。采用BBB评分法评估大鼠后肢运动功能变化

免疫组化法检测各组大鼠脊髓组织中RhoA、ROCKⅡ、MLC蛋白的表达情况。结果:BBB评分结果显示:与假手术组比较

造模后14d、28d模型组大鼠运动功能评分显著降低(P<0.05);与模型组比较

造模后14d、28d夹脊电针组、抑制剂组大鼠运动功能评分均显著升高(P<0.05);夹脊电针组、抑制剂组造模后28d大鼠肢体运动功能评分较造模后14d显著升高(P<0.05)。免疫组化结果显示:与假手术组比较

造模后14d、28d模型组大鼠脊髓组织RhoA、ROCKⅡ、MLC阳性细胞数增加(P<0.05);与模型组相比

造模后14d、28d夹脊电针组、抑制剂组大鼠脊髓组织RhoA、ROCKⅡ、MLC阳性细胞数显著降低(P<0.05);夹脊电针组、抑制剂组造模后28d大鼠脊髓组织RhoA、ROCKⅡ、MLC阳性细胞数较造模后14d显著降低(P<0.05)。结论:夹脊电针能够显著改善ASCI大鼠肢体运动功能

其作用机制可能与抑制大鼠脊髓损伤组织微环境Rho-ROCKⅡ信号通路RhoA、ROCKⅡ、MLC抑制因子表达有关。

Abstract

Objective To observe the effect of electroacupuncture(EA)of"Jiaji"(EX-B 2)on limb locomotor function and expression of Ras homolog gene family member A(RhoA)

Rho-associated kinase Ⅱ(ROCK Ⅱ)and myosin light chain(MLC)proteins in the anterior horn of spinal cord in acute spinal cord injury(ASCI)rats

so as to explore its mechanisms underlying improvement of SCI-induced limb locomotor dysfunction.Methods Forty-eight female Wistar rats were randomly divided into sham operation(sham)

ASCI model(model)

EA EX-B 2(EA)and ROCK inhibitor(Fasudil)groups which were further divided into 14 dand 28 dsubgroups(n=6 in each).The ASCI model was made by using weight drop striking method.Three hours after modeling

EA(100 Hz

0.4

0.6 mA)was applied to EX-B 2(T 9

T 11)for 30 min

once daily for 14 dand 28 d

respectively.The ROCK inhibitor(hydrochloride Fasudil

10 mg/kg)was administrated by intraperitoneal injection immediately after modeling

once a day

continuously for 14 dor 28 d.The expression of RhoA

ROCK Ⅱ and MLC proteins in the spinal cord anterior horn tissue(T 10)was detected by immunohistochemistry.The rats' hindlimb locomotor function was assessed according to Basso

Beattie and Bresnahan(BBB)locomotor rating scale(21-points).Results After ASCI

the BBB scores were significantly lower in the model group than in the sham group on day 14 and 28(P<0.05)

and obviously higher in the EA and inhibitor groups than in the model group(P<0.05)

suggesting an improvement of the hindlimb locomotor function after EA intervention or suppression of ROCK.Immunohistochemical results indicated that the numbers of RhoA

ROCK Ⅱ and MLC immune-reaction positive cells in the anterior horn of spinal cord were significantly more in the model group than in the sham group(P<0.05)

and remarkably decreased in both EA and inhibitor groups on day 14 and 28 relevant to the model group(P<0.05).The therapeutic effects of EA were markedly weaker than those of inhibitor Fasudil in up-regulating BBB score and down-regulating the number of RhoA

ROCKⅡ and MLC positive cells(P<0.05).Conclusion EA of EX-B 2 can improve the hindlimb locomotor function in ASCI rats

which may be associated with its effect in down-regulating the expression of RhoA

ROCK Ⅱ and MLC proteins(i.e.

inhibiting the RhoA/ROCK signaling pathway)in the anterior horn of spinal cord.

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