Electroacupuncture improves denervated gastrocnemius atrophy by regulating expression of forkhead protein 3A,muscle atrophy F-box and myogenic differentiation antigen proteins in sciatic nerve injury rats

WU Meng-jia; TANG Cheng-lin; HUANG Si-qin; ZHAO Dan-dan; LUO Ao; ZHANG An-ning; AN Huiyu; TAN Cheng-fang; QIU Li

doi:10.13702/j.1000-0607.171015

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Electroacupuncture improves denervated gastrocnemius atrophy by regulating expression of forkhead protein 3A,muscle atrophy F-box and myogenic differentiation antigen proteins in sciatic nerve injury rats

更新时间：2023-08-11

- Electroacupuncture improves denervated gastrocnemius atrophy by regulating expression of forkhead protein 3A,muscle atrophy F-box and myogenic differentiation antigen proteins in sciatic nerve injury rats
- Acupuncture Research Vol. 44, Issue 4, Pages: 253-257(2019)
- 作者机构：
  
  1. 重庆医科大学中医药学院
  2. 重庆医科大学中医药防治代谢性疾病重庆市重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.171015
  CLC：
- Published：2019
- 稿件说明：
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WU Meng-jia, TANG Cheng-lin, HUANG Si-qin, et al. Electroacupuncture improves denervated gastrocnemius atrophy by regulating expression of forkhead protein 3A,muscle atrophy F-box and myogenic differentiation antigen proteins in sciatic nerve injury rats[J]. Acupuncture research, 2019, 44(4): 253-257.
DOI：

WU Meng-jia, TANG Cheng-lin, HUANG Si-qin, et al. Electroacupuncture improves denervated gastrocnemius atrophy by regulating expression of forkhead protein 3A,muscle atrophy F-box and myogenic differentiation antigen proteins in sciatic nerve injury rats[J]. Acupuncture research, 2019, 44(4): 253-257. DOI： 10.13702/j.1000-0607.171015.

摘要

目的:观察电针干预对失神经肌萎缩大鼠腓肠肌中叉头蛋白转录因子3A(fork-head protein

FOXO3A)、肌萎缩F-box蛋白(muscle atrophy F-box

MAFbx)和成肌分化抗原(myogenic differentiation antigen

Myod1)蛋白及基因表达的影响

探讨电针延缓大鼠失神经肌萎缩的可能机制。方法:雄性Sprague-Dawley大鼠随机分为假手术组、模型组及电针组

每组6只。采用钳夹坐骨神经法制备大鼠失神经腓肠肌萎缩模型。电针组选取大鼠术侧"足三里""环跳"穴进行治疗

每日1次

每次10min

连续干预14d后取材。计算各组大鼠腓肠肌湿重比

HE染色测定大鼠术侧腓肠肌纤维截面积和直径

Western blot检测大鼠术侧腓肠肌中FOXO3A、MAFbx、Myod1蛋白的相对表达量

实时荧光定量PCR检测大鼠术侧腓肠肌中FOXO3A、MAFbx及Myod1mRNA的相对表达量。结果:与假手术组相比

模型组大鼠腓肠肌湿重比、肌纤维截面积和直径均显著下降(P<0.01)

电针组高于模型组(P<0.01)。与假手术组相比

模型组大鼠腓肠肌中FOXO3A、MAFbx、Myod1蛋白及mRNA表达量显著升高(P<0.01);与模型组相比

电针干预后FOXO3A、MAFbx蛋白及mRNA表达量显著降低(P<0.01)

Myod1蛋白及mRNA表达量显著升高(P<0.01)。结论:电针可能通过抑制FOXO3A、MAFbx的表达同时上调Myod1的表达

影响骨骼肌蛋白水解的速率和肌卫星细胞分化的水平

在延缓失神经肌萎缩的同时促进肌萎缩修复。

Abstract

Objective To observe the effect of electroacupuncture(EA)on morphological changes of denervated gastrocnemius(GS)and the expression of fork-head protein(FOXO3 A)

muscle atrophy F-box(MAFbx)and myogenic differentiation antigen(Myod1)in sciatic nerve injury rats

so as to reveal its mechanism underlying improvement of myoatrophy.Methods Eighteen male Sprague-Dawley rats were randomly divided into sham operation

model and EA groups(n=6 per group).The model of gastrocnemius atrophy was established by crushing the right sciatic nerve.Then

EA(2 Hz)was applied to the right"Zusanli"(ST36)and"Huantiao"(GB30)for 10 min

once a day for 14 successive days.The wet weight of the GS on both sides was weighted to calculate the wet weight ratio(the injured side/the healthy side)

and the cross-sectional area(CSA)and diameter of GS fibers were measured after H.E.staining.The expressions of FOXO3 A

MAFbx and Myod1 protein and mRNA in the GS tissue were tested using Western blot and fluorescence quantitative PCR

separately.Results Following modeling

the GS wet weight ratio

CSA and fiber diameter were smaller in the model group than those in the sham group(P<0.01)

and were significantly higher in the EA group than in the model group(P<0.01).H.E.staining showed that the GS fibers became smaller and the myocyte got round in the model group

while the GS fibers were bigger and the myocyte was relatively regular in morphology in the EA group.After modeling

the expression levels of FOXO3 A

MAFbx and Myod1 mRNA and protein were evidently higher in the model group(P<0.01);Moreover

after EA treatment

modeling-induced increasing of expression levels of FOXO3 Aand MAFbx mRNA and protein were revised(P<0.01)

while the increased expression level of Myod1 was further up-regulated relavant to that in the model group(P<0.01).Conclusion EA of ST36 and GB30 can suppress the up-regulated expression of FOXO3 Aand MAFbx mRNA and protein and further promote the expression of Myod1 mRNA and protein in the GS tissue in rats with denervated GS atrophy

which may contribute to its function in relieving the myoatrophy

promoting the skeletal muscle protein hydrolysis and differentiation of satellite cells.

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