Electroacupuncture improves intestinal function by down-regulating expression of 5-HT_(1A)R and c-fos proteins in the hypothalamus and colon tissues via brain-gut axis in rats with functional diarrhea

WANG Qiang; WANG Yuan; NIU Wen-min; YANG Xiao-hang; LIU Zhi-bin

doi:10.13702/j.1000-0607.180023

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Electroacupuncture improves intestinal function by down-regulating expression of 5-HT_(1A)R and c-fos proteins in the hypothalamus and colon tissues via brain-gut axis in rats with functional diarrhea

更新时间：2023-08-11

- Electroacupuncture improves intestinal function by down-regulating expression of 5-HT_(1A)R and c-fos proteins in the hypothalamus and colon tissues via brain-gut axis in rats with functional diarrhea
- Acupuncture Research Vol. 44, Issue 7, Pages: 501-505(2019)
- 作者机构：
  
  1. 陕西中医药大学针灸推拿学院
  2. 陕西省针药结合重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.180023
  CLC：
- Published：2019
- 稿件说明：
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WANG Qiang, WANG Yuan, NIU Wen-min, et al. Electroacupuncture improves intestinal function by down-regulating expression of 5-HT_(1A)R and c-fos proteins in the hypothalamus and colon tissues via brain-gut axis in rats with functional diarrhea[J]. Acupuncture research, 2019, 44(7): 501-505.
DOI：

WANG Qiang, WANG Yuan, NIU Wen-min, et al. Electroacupuncture improves intestinal function by down-regulating expression of 5-HT_(1A)R and c-fos proteins in the hypothalamus and colon tissues via brain-gut axis in rats with functional diarrhea[J]. Acupuncture research, 2019, 44(7): 501-505. DOI： 10.13702/j.1000-0607.180023.

摘要

目的:通过观察电针不同组穴对功能性腹泻(FD)大鼠下丘脑及结肠组织5-羟色胺1A受体(5-HT_(1A)R)、c-fos蛋白表达的影响

初步探讨电针对FD大鼠脑-肠关系的影响。方法:雄性SD大鼠随机分为空白组、模型组、电针Ⅰ组、电针Ⅱ组

每组10只。采用番泻叶加束缚制动的方法建立FD大鼠模型。电针Ⅰ组电针大鼠双侧"天枢""大肠俞"

电针Ⅱ组电针大鼠双侧"曲池""上巨虚"

均留针20min

每日1次

连续10d。观察并记录各组大鼠治疗前后稀便率及腹泻指数。Western blot法检测各组大鼠下丘脑及结肠组织5-HT_(1A)R、c-fos蛋白表达水平。结果:治疗前

与空白组比较

模型组、电针Ⅰ组、电针Ⅱ组稀便率及腹泻指数均升高(P<0.01)。治疗后

与空白组比较

模型组稀便率及腹泻指数均升高(P<0.01);与模型组比较

电针Ⅰ组稀便率及腹泻指数均降低(P<0.01);与电针Ⅰ组比较

电针Ⅱ组稀便率及腹泻指数显著升高(P<0.01)。与空白组比较

模型组下丘脑及结肠组织中5-HT_(1A)R和c-fos蛋白表达水平均升高(P<0.05);与模型组比较

电针Ⅰ组下丘脑及结肠组织中5-HT_(1A)R及c-fos蛋白表达水平降低(P<0.05)

电针Ⅱ组结肠组织中5-HT_(1A)R蛋白表达水平降低(P<0.05);与电针Ⅰ组比较

电针Ⅱ组大鼠下丘脑中5-HT_(1A)R、下丘脑及结肠组织中c-fos蛋白表达水平均升高(P<0.05)。结论:电针"天枢""大肠俞"可减轻番泻叶诱导的FD大鼠的腹泻症状

降低下丘脑及结肠组织5-HT_(1A)R和c-fos的表达

其机制可能与脑-肠轴系统有关。

Abstract

Objective To observe the effect of electroacupuncture(EA)on expression of 5-HT_(1A)receptor(5-HT_(1A)R and c-fos proteins in the hypothalamus and colon tissues in functional diarrhea(FD)rats so as to explore its underlying mechanisms in improving intestinal function via brain-gut axis.Methods A total of 40 SD rats were randomized into blank control

model

EA Tianshu(ST25)-Dachangshu(BL25

ST25-BL25)and EA Quchi(LI11)-Shangjuxu(ST37

LI11-ST37)groups.The FD model was established by gavage of Folium Sennae(10 mg/kg)and constraint immobilization once daily for 29 days.EA(10 Hz/50 Hz

1.5 mA)was applied to bilateral ST25 and BL25 in EA ST25-BL25 group

and bilateral LI11 and ST37 in EA LI11-ST37 group for20 min

once daily for successive 10 days.The expression of 5-HT_(1A)R and c-fos protein in the hypothalamus and colon tissues was determined by using Western blot.The state of stool was recorded for calculating the loose stool rate and diarrhea index.Results After modeling

the loose stool rate

diarrhea index and expression levels of 5-HT_(1A)R and c-fos proteins in the colon and hypothalamus tissues were obviously increased in the model group in contrast with the blank control group(P<0.01

P<0.05).Following EA intervention

the loose stool rate and diarrhea index

the expression levels of 5-HT_(1A)R and c-fos proteins in the hypothalamus and colon in the EA ST25-BL25 group

and the expression of 5-HT_(1A)R in the colon in the EA LI11-ST37 group were significantly downregulated relevant to the model group(P<0.01

P<0.05).No significant changes were found in loose stool rate and diarrhea index

and the expression levels of hypothalamic 5-HT_(1A)R and hypothalamic and colonic c-fos proteins in the EA LI11-ST37 group(P>0.05).The expression levels of 5-HT_(1A)R protein in the hypothalamus and c-fos protein in the hypothalamus and colon in the EA LI11-ST37 group were significantly up-regulated relevant to the EA ST25-BL25 group(P<0.05).Conclusion EA of ST25-BL25 can down-regulate expression of 5-HT_(1A)R and c-fos proteins in the hypothalamus and colon tissue in FD rats

which may contribute to its function in improving symptoms of FD possibly via brain-gut axis.

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