Effect of moxibustion at “Zusanli” (ST36) on metabolites of gastric tissue in rats with chronic atrophic gastritis based on metabonomics

LIU Xia; SHE Chang; ZHONG Huan; LIU Tao; CAO Jia-nan; ZHANG Chi; LIU Mi; CHANG Xiao-rong

doi:10.13702/j.1000-0607.180149

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Effect of moxibustion at “Zusanli” (ST36) on metabolites of gastric tissue in rats with chronic atrophic gastritis based on metabonomics

更新时间：2023-08-11

- Effect of moxibustion at “Zusanli” (ST36) on metabolites of gastric tissue in rats with chronic atrophic gastritis based on metabonomics
- Acupuncture Research Vol. 44, Issue 2, Pages: 113-119(2019)
- 作者机构：
  
  1. 湖南中医药大学针灸推拿学院
  2. 长沙市中医医院(长沙市第八医院)
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.180149
  CLC：
- Published：2019
- 稿件说明：
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LIU Xia, SHE Chang, ZHONG Huan, et al. Effect of moxibustion at “Zusanli” (ST36) on metabolites of gastric tissue in rats with chronic atrophic gastritis based on metabonomics[J]. Acupuncture research, 2019, 44(2): 113-119.
DOI：

LIU Xia, SHE Chang, ZHONG Huan, et al. Effect of moxibustion at “Zusanli” (ST36) on metabolites of gastric tissue in rats with chronic atrophic gastritis based on metabonomics[J]. Acupuncture research, 2019, 44(2): 113-119. DOI： 10.13702/j.1000-0607.180149.

摘要

目的:采用代谢组学的方法观察正常及疾病状态下

艾灸"足三里"对胃组织代谢产物的影响

从代谢组学角度探讨阳明经穴位与胃之间相对特异性联系的基础。方法:将56只SD大鼠分为正常组、正常+艾灸组、模型组、模型+艾灸组

每组14只。通过N-甲基-N′-硝基-N-亚硝基胍联合乙醇灌胃、饥饱失常法复制慢性萎缩性胃炎(CAG)大鼠模型。正常组和模型组不予以治疗

正常组+艾灸组和模型组+艾灸组予以艾灸"足三里""中脘"穴治疗

每次15min

每日1次

共2周。HE染色观察大鼠胃组织病理变化

核磁共振氢谱(1 H NMR)技术检测胃组织代谢物并用Chenomx NMR Suite软件分析所得数据。结果:模型组大鼠胃组织病理改变符合CAG病理诊断标准

模型+艾灸组大鼠胃组织病理改变较模型组有一定程度的改善。与正常组比较

模型组大鼠胃组织中的乳酸、谷胱甘肽、N-乙酰天门冬氨酸、尿苷二磷酸葡萄糖、肌糖升高(P<0.05)

亮氨酸、甜菜碱、磷酸胆碱、尿嘧啶降低(P<0.05);艾灸"足三里"后浓度发生逆转的代谢物有谷胱甘肽、N-乙酰天门冬氨酸、磷酸胆碱、尿嘧啶(和模型组比较

P<0.05)。与正常组比较

正常+艾灸组胃组织中的肌醇升高(P<0.05)

而3-羟基丁酸、丝氨酸、尿苷二磷酸葡萄糖降低(P<0.05)。结论:从代谢组学角度证明了胃经穴位与胃之间存在一定的相对特异性联系

谷胱甘肽、N-乙酰天门冬氨酸、磷酸胆碱、尿嘧啶等差异性代谢物可能是介导艾灸胃经穴位产生效应的代谢物质基础。

Abstract

Objective To observe the effect of moxibustion at"Zusanli"(ST36)on metabolites of gastric tissue in chronic atrophic gastritis(CAG)rats

so as to explore the correlation between Yangming Meridian and stomach based on metabolomics.Methods Fifty-six SD rats were randomly divided into normal

normal+ moxibustion

model and model+ moxibustion groups(n=14 rats/group).The CAG model was established by gavage of N-methyl-N′-nitro-N-nitroso-guanidine(MNNG)solution and irregular diet for 12 weeks.Moxibustion was applied to ST36 and"Zhongwan"(CV12)for 15 min

once daily for two weeks in the normal+ moxibustion group and model+moxibustion group.The histopathological changes of the gastric tissue were observed by H.E.staining.1 H nuclear magnetic resonance(1 H NMR)spectroscopy was used to detect gastric tissue metabolites and the data were analyzed using the Chenomx NMR Suite software.Results After modeling

the regional gastric tissue showed pale thinner gastric wall

and reduction of regional glands with necrosis and disordered arrangement

epithelial cell exfoliation

lymphocyte infiltration as well as scattered punctate hemorrhage

which was evidently milder in the model+ moxibustion group.The metabolites of lactic acid

glutathione

N-acetylaspartate

uridine diphosphate glucose and muscle sugar in the gastric tissue in the model group were increased compared with those in the normal group(P<0.05)

and the metabolites of leucine

betaine

phosphorylcholine

and uracil were decreased(P<0.05).The glutathione

N-acetylaspartate

phosphorylcholine and uracil in the model+moxibustion group were significantly reversed relevant to the model group(P<0.05).The inositol in gastric tissue were significantly increased(P<0.05)

3-hydroxybutyric acid

serine and uridine diphosphate glucose were significantly decreased(P<0.05)in the normal+ moxibustion group than in the normal group.Conclusion Moxibustion has a good efficacy in regulating some metabolic substances such as glutathione

N-acetylaspartic acid

choline phosphate and uracil of CAG rats

suggesting a specific relationship between the Stomach Meridian and the stomach.

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Related Institution

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