Effect of electroacupuncture on myocardial infarct size and expression of inflammatory cytokines and sympathetic-active substances in the myocardial ischemic tissue of rats

LU Sheng-feng; YUAN Jing; DING Ya-juan; YU Mei-ling; FU Shu-ping; HONG Hao; CAI Yun; ZHU Bing-mei

doi:10.13702/j.1000-0607.180210

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Effect of electroacupuncture on myocardial infarct size and expression of inflammatory cytokines and sympathetic-active substances in the myocardial ischemic tissue of rats

更新时间：2023-08-11

- Effect of electroacupuncture on myocardial infarct size and expression of inflammatory cytokines and sympathetic-active substances in the myocardial ischemic tissue of rats
- Acupuncture Research Vol. 44, Issue 5, Pages: 313-318(2019)
- 作者机构：
  
  1. 南京中医药大学针药结合教育部重点实验室
  2. 四川大学华西医院再生医学研究中心
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.180210
  CLC：
- Published：2019
- 稿件说明：
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LU Sheng-feng, YUAN Jing, DING Ya-juan, et al. Effect of electroacupuncture on myocardial infarct size and expression of inflammatory cytokines and sympathetic-active substances in the myocardial ischemic tissue of rats[J]. Acupuncture research, 2019, 44(5): 313-318.
DOI：

LU Sheng-feng, YUAN Jing, DING Ya-juan, et al. Effect of electroacupuncture on myocardial infarct size and expression of inflammatory cytokines and sympathetic-active substances in the myocardial ischemic tissue of rats[J]. Acupuncture research, 2019, 44(5): 313-318. DOI： 10.13702/j.1000-0607.180210.

摘要

目的:观察电针对缺血心肌组织中白细胞介素(IL)-8、IL-10和酪氨酸羟化酶(TH)、β3肾上腺素能受体(β3AR)、内皮型一氧化氮合酶(eNOS)表达的影响

探讨电针促心肌保护与抗炎及调控交感神经重塑的相关性。方法:SD大鼠随机分成假手术组(9只)、假手术电针组(9只)、模型组(15只)、电针组(15只)。结扎冠状动脉左前降支复制心肌缺血(MI)模型。假手术电针组和电针组电针双侧"内关"穴

每次30min

每日1次

治疗4d。采用TTC染色检测大鼠心肌梗死面积百分比

HE染色观察心肌组织形态和炎性浸润

Western blot法检测心肌组织中IL-8、IL-10、TH、β3AR、eNOS的表达。结果:与假手术组比较

模型组大鼠心肌梗死明显、心肌纤维形态紊乱

并有大量的炎性细胞浸润

心肌组织中IL-8、TH、β3AR、eNOS表达增加(P<0.01)

IL-10表达变化不明显(P>0.05)。与模型组比较

电针组大鼠心肌梗死面积减小(P<0.01)、心肌炎性细胞浸润减少、心肌纤维形态明显改善

心肌组织中IL-10表达上升(P<0.05)

IL-8、TH、β3AR表达下降(P<0.01)

eNOS表达上升(P<0.05)。结论:电针可能通过调控炎性反应和交感神经重塑

减小心肌梗死面积

产生心肌保护效应。

Abstract

Objective To investigate the effect of electroacupuncture(EA)on the expression of interleukin-8(IL-8)

interleukin-10(IL-10)

tyrosine hydroxylase(TH)

β3-adrenergic receptor(β3 AR)

and endothelial nitric oxide synthase(eNOS)in myocardial tissue in ischemic myocardial injury rats

so as to reveal its underlying mechanisms in myocardial protection via antiinflammation and sympathetic nerve remodeling.Methods A total of 48 male Sprague-Dawley rats were randomly divided into sham-operation(sham

n=9)

sham +EA(n=9)

model(n=15)and EA(n=15)groups.The myocardial ischemia(MI)model was established by ligation of the left anterior descending branch of the left coronary artery.EA(2 Hz/15 Hz

1.5-2 mA)was applied to bilateral"Neiguan"(PC6)for 30 min

once daily for 4 days.The myocardial infarct size was detected by 2

5 triphenyltetrazolium chloride(TTC)staining

myocardial histopathological changes and inflammatory infiltration were assessed by H.E.staining

and the expression of IL-8

IL-10

β3 AR

and eNOS in the myocardium was determined by using Western blot.Results Compared with the sham group

a marked myocardial infarction was found in the left ventricle tissue

accompanied with disordered arrangement of myocardial fibers and higher degree of inflammatory cell infiltration

and increased expression of IL-8

β3 AR and eNOS in the myocardium in the model group(P<0.01)

but without significant change in the expression of IL-10(P>0.05).After EA intervention and in comparison with the model group

the myocardial infarct size was significantly reduced(P<0.01)

the severity of inflammatory cell infiltration and disordered arrangement of myocardial fibers were relieved

and the expression of IL-10 and eNOS proteins were significantly up-regulated(P<0.05)

and the markedly up-regulated expression of IL-8

andβ3 AR were significantly suppressed in the EA group(P<0.01).Conclusion EA intervention can reduce the myocardial infarct size(protective effect)in MI rats possibly by reducing inflammatory reaction and sympathetic nerve remodeling.

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