Electroacupuncture of Multiple Acupoints Is Significantly Superior to That of Single Acupoint in Improving Sleep by Regulating Serum Sleep-promoting and Awakening-promoting Factors in Insomnia Rats

QIAO Li-na; GAO Qiao-ling; TAN Lian-hong; SHI Yi-nan; JIANG Yan-shu; YANG Jiao-jiao; YANG Yong-sheng

doi:10.13702/j.1000-0607.180317

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Electroacupuncture of Multiple Acupoints Is Significantly Superior to That of Single Acupoint in Improving Sleep by Regulating Serum Sleep-promoting and Awakening-promoting Factors in Insomnia Rats

更新时间：2023-08-10

- Electroacupuncture of Multiple Acupoints Is Significantly Superior to That of Single Acupoint in Improving Sleep by Regulating Serum Sleep-promoting and Awakening-promoting Factors in Insomnia Rats
- Acupuncture Research Vol. 43, Issue 10, Pages: 651-656(2018)
- 作者机构：
  
  1. 中国中医科学院针灸研究所
  2. 长春中医药大学针灸推拿学院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.180317
  CLC：
- Published：2018
- 稿件说明：
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QIAO Li-na, GAO Qiao-ling, TAN Lian-hong, et al. Electroacupuncture of Multiple Acupoints Is Significantly Superior to That of Single Acupoint in Improving Sleep by Regulating Serum Sleep-promoting and Awakening-promoting Factors in Insomnia Rats[J]. Acupuncture research, 2018, 43(10): 651-656.
DOI：

QIAO Li-na, GAO Qiao-ling, TAN Lian-hong, et al. Electroacupuncture of Multiple Acupoints Is Significantly Superior to That of Single Acupoint in Improving Sleep by Regulating Serum Sleep-promoting and Awakening-promoting Factors in Insomnia Rats[J]. Acupuncture research, 2018, 43(10): 651-656. DOI： 10.13702/j.1000-0607.180317.

摘要

目的:通过观察电针刺激单穴与腧穴配伍对对氯苯丙氨酸(PCPA)致失眠大鼠血清内源性睡眠-觉醒调节相关因子白介素-1β(IL-1β)、脑源性神经生长因子(BDNF)、前列腺素D2(PGD2)、褪黑激素(MLT)和皮质酮(CORT)含量的影响

探讨电针单穴与腧穴配伍对失眠的调节效应差异及其作用机制。方法:SD大鼠随机分为正常组、模型组、神门组、百会组、三阴交组、神门-百会-三阴交组(配伍组)

每组9只。采用连续2d腹腔注射PCPA悬浊液(300 mg/kg)建立失眠大鼠模型

各治疗组分别电针"百会"或双侧"神门""三阴交""神门+百会+三阴交"穴

每次30min

1次/d

连续4d。治疗后比较各组大鼠睡眠潜伏期(SL)和睡眠时间(ST);ELISA法检测血清IL-1β、BDNF、MLT、PGD2和CORT的含量。结果:与正常组相比

模型组SL明显延长(P<0.01)

ST显著缩短(P<0.01);与模型组比较

神门组、百会组与配伍组的SL均显著缩短(P<0.05

P<0.01)

各单穴组与配伍组ST明显延长(P<0.05

P<0.01);与各单穴组相比

配伍组大鼠的SL明显缩短、ST显著延长(P<0.05)。与正常组相比

模型组血清IL-1β、BDNF、PGD2和MLT的含量显著降低(P<0.05)

CORT的含量显著升高(P<0.05);与模型组相比

单穴组和配伍组血清IL-1β、BDNF、PGD2和MLT的含量显著升高(P<0.05)

CORT的含量明显降低(P<0.05);配伍组IL-1β、BDNF、PGD2的含量显著高于百会组和三阴交组(P<0.05)

MLT的浓度显著高于所有单穴组(P<0.05)

CORT的浓度明显低于神门组和三阴交组(P<0.05)。结论:电针单穴"神门""百会"或"三阴交"和腧穴配伍均可改善大鼠的睡眠状况

此效应可能与提高血清中IL-1β等促睡眠因子和下调促觉醒因子CORT的含量有关

并且腧穴配伍应用的调节作用总体上要优于单穴。

Abstract

Objective To observe the effect of electroacupuncture(EA)stimulation of single and multiple acupoints on sleep and concentrations of interlukin-1β(IL-1β)

brain-derived neurotrophic factor(BDNF)

prostaglandin D2(PGD2)and melatonin(MLT

sleep-promoting factors)and corticosterone(CORT

awakening-promoting factor)in the serum in insomnia rats

so as to explore its efficacy difference and the mechanism underlying improving sleep.Methods Fifty-four male SD rats were randomly divided into control

model

EA-Baihui(GV 20)

EA-Shenmen(HT 7)

EA-Sanyinjiao(SP 6)and EA-GV 20+HT 7+SP 6 groups(n=9 rats in each group).The insomnia model was established by intraperitoneal injection of para-chlorophenylalanine(PCPA

300 mg/kg)once daily for 2 days.In the EA-GV 20

EA-HT 7

EA-SP 6 and EA-GV 20+HT 7+SP 6 groups

EA stimulation was administrated for 30 min

once a day for 4 days.The sleep onset latency and sleep duration were measured after intraperitoneal injection of pentobarbital sodium(35 mg/kg).The concentrations of IL-1β

BDNF

MLT

PGD2 and CORT in the serum were detected by ELISA.Results After EA stimulation of GV 20

HT 7

SP 6 and GV 20+HT 7+SP 6

the sleep latency was significantly shortened(P<0.05

P<0.01

except SP 6)

and the sleep duration was remarkably prolonged in comparison with the model group(P<0.05

P<0.01)

and the therapeutic effects of EA-GV 20+HT 7+SP 6 were significantly superior to those of EA-GV 20

EA-HT 7 and EA-SP 6 in shortening the sleep latency and lengthening the sleep duration(P<0.05).Following modeling

the concentrations of IL-1β

BDNF

PGD2 and MLT were significantly down-regulated

and the CORT level was markedly upregulated in the model group relevant to the control group(P<0.05).Following EA

modeling induced dramatic decrease of serum IL-1β

BDNF

PGD2 and MLT was considerably up-regulated

and the increased CORT level markedly down-regulated in the EA-GV 20

EA-HT 7

EA-SP 6 and EA-GV 20+HT 7+SP 6 groups(P<0.05).The effects of EA-GV 20+HT 7+SP 6 were evidently superior to those of EA-GV 20 and EA-SP 6 in up-regulating serum IL-1β

BDNF and PGD2 levels

and to those of HT 7

GV20 and SP 6 in up-regulating serum MLT level

and significantly superior to those of EA-ST 7 and EA-SP 6 in down-regulating serum CORT(P<0.05).Conclusion EA stimulation of HT 7

GV 20

SP 6 and GV 20+HT 7+ SP 6 can significantly improve the sleep in insomnia rats

which is closely associated with its effects in regulating serum sleep-promoting factors and awakeningpromoting factor.Joint administration of EA of GV 20+HT 7+ SP 6 has a better effect than the single acupoint mentioned above.

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