Effect of moxibustion on inflammatory cytokine levels in hippocampus and hypothalamus in rats with fatigue

LI Tian-ge; PU Rong; SHUI Ling; BAI Shu-mei; LU Jun; CHEN Ying-song; TU Ya

doi:10.13702/j.1000-0607.180619

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Effect of moxibustion on inflammatory cytokine levels in hippocampus and hypothalamus in rats with fatigue

更新时间：2023-08-11

- Effect of moxibustion on inflammatory cytokine levels in hippocampus and hypothalamus in rats with fatigue
- Acupuncture Research Vol. 44, Issue 3, Pages: 195-199(2019)
- 作者机构：
  
  1. 北京中医药大学针灸推拿学院
  2. 内蒙古医科大学蒙医药学院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.180619
  CLC：
- Published：2019
- 稿件说明：
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LI Tian-ge, PU Rong, SHUI Ling, et al. Effect of moxibustion on inflammatory cytokine levels in hippocampus and hypothalamus in rats with fatigue[J]. Acupuncture research, 2019, 44(3): 195-199.
DOI：

LI Tian-ge, PU Rong, SHUI Ling, et al. Effect of moxibustion on inflammatory cytokine levels in hippocampus and hypothalamus in rats with fatigue[J]. Acupuncture research, 2019, 44(3): 195-199. DOI： 10.13702/j.1000-0607.180619.

摘要

目的:观察艾灸对疲劳大鼠不同脑区炎性细胞因子的影响

探讨艾灸缓解疲劳的可能机制。方法:SD大鼠随机分为正常组、模型组和艾灸组

每组8只。采用慢性负重力竭游泳法建立疲劳大鼠模型。艾灸组在造模同时灸双侧"足三里"穴各3壮

隔日1次

共11次。观察大鼠力竭游泳时间

ELISA法检测大鼠海马和下丘脑炎性细胞因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和γ-干扰素(IFN-γ)的含量。结果:与模型组比较

艾灸组在造模第14天和第21天力竭游泳时间显著延长(P<0.05

P<0.01)。与正常组比较

模型组大鼠海马IL-1β、IL-6和TNF-α含量显著升高(P<0.01)

IFN-γ含量显著降低(P<0.05);模型组大鼠下丘脑IL-1β、IL-6、TNF-α和IFN-γ含量均呈增加趋势

但差异无统计学意义(P>0.05)。与模型组比较

艾灸组大鼠海马IL-6含量显著降低(P<0.01)

IL-1β、TNF-α、IFN-γ含量差异无统计学意义(P>0.05);艾灸组大鼠下丘脑IL-1β、IL-6、TNF-α和IFN-γ含量差异无统计学意义(P>0.05)。结论:艾灸可显著下调大鼠海马促炎细胞因子水平

对下丘脑促炎细胞因子无明显作用。艾灸降低中枢炎性细胞因子与其发挥缓解疲劳作用的机制值得进一步研究。

Abstract

Objective To investigate the effect of moxibustion of"Zusanli"(ST36)on levels of inflammatory cytokines in the hippocampus and hypothalamus regions of rats with fatigue

so as to reveal its anti-fatigue mechanism.Methods Twentyfour male Sprague-Dawley rats were randomly divided into normal control

fatigue model

and moxibustion groups

with 8 rats in each group.The fatigue model was established by chronic weight-loaded exhaustive swimming.During modeling

the rats in the moxibustion group were given moxibustion(3 moxa cones

about 8 min in duration)at bilateral ST36

once every other day for11 times in total.The duration of exhausted swimming was observed

and ELISA was used to measure the contents of interleukin-1β(IL-1β)

interleukin-6(IL-6)

tumor necrosis factor-α(TNF-α)

and interferon gamma(IFN-γ)in the hippocampus and hypothalamus tissues.Results The duration of exhausted swimming was obviously prolonged on day 14 and 21 after moxibustion intervention relevant to the model group(P<0.05

P<0.01).The contents of IL-1β

IL-6 and TNF-αin the hippocampus were significantly increased in the model group than in the normal group(P<0.01)

and that of hippocampal IL-6 was considerably decreased in the moxibustion group than in the model group(P<0.05).The content of hippocampal IFN-γwas significantly lower in the model group than in the normal group(P<0.05)and was increased mildly after moxibustion(P>0.05).No significantly changes were found in the levels of hypothalamic IL-1β

IL-6

TNF-αand IFN-γafter modeling and moxibustion(P>0.05)

and in the levels of hippocampal IL-1β

TNF-αand IFN-γafter moxibustion(P>0.05).Conclusion Moxibustion can significantly down-regulate hippocampal IL-6 content in fatigue rats

which maybe contribute to its effect in alleviating fatigue.Further studies need being conducted to identify this conclusion.

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Related Institution

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