Comparison of effects of electroacupuncture at “Tianshu” (ST25) and “Dachangshu” (BL25) on intestinal sensitivity and expression of muscarinic M_3R and 5-HT_(3A)R in irritable bowel syndrome rats

JI Mao-xian; GUO Meng-wei; GAO Yu-shan; LAN Ying; WANG Shan; WANG Yi-fan; QIN Ying; ZHANG Hong-lin; REN Xiao-xuan

doi:10.13702/j.1000-0607.180764

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Comparison of effects of electroacupuncture at “Tianshu” (ST25) and “Dachangshu” (BL25) on intestinal sensitivity and expression of muscarinic M_3R and 5-HT_(3A)R in irritable bowel syndrome rats

更新时间：2023-08-11

- Comparison of effects of electroacupuncture at “Tianshu” (ST25) and “Dachangshu” (BL25) on intestinal sensitivity and expression of muscarinic M_3R and 5-HT_(3A)R in irritable bowel syndrome rats
- Acupuncture Research Vol. 44, Issue 4, Pages: 264-269(2019)
- 作者机构：
  
  1. 北京中医药大学针灸推拿学院
  2. 北京中医药大学中医学院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.180764
  CLC：
- Published：2019
- 稿件说明：
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JI Mao-xian, GUO Meng-wei, GAO Yu-shan, et al. Comparison of effects of electroacupuncture at “Tianshu” (ST25) and “Dachangshu” (BL25) on intestinal sensitivity and expression of muscarinic M_3R and 5-HT_(3A)R in irritable bowel syndrome rats[J]. Acupuncture research, 2019, 44(4): 264-269.
DOI：

JI Mao-xian, GUO Meng-wei, GAO Yu-shan, et al. Comparison of effects of electroacupuncture at “Tianshu” (ST25) and “Dachangshu” (BL25) on intestinal sensitivity and expression of muscarinic M_3R and 5-HT_(3A)R in irritable bowel syndrome rats[J]. Acupuncture research, 2019, 44(4): 264-269. DOI： 10.13702/j.1000-0607.180764.

摘要

目的:观察电针"天枢"和"大肠俞"对肠易激综合征(IBS)大鼠的肠道压力值和敏感性的影响

并探讨其效应差异及相关机制。方法:新生Wistar大鼠40只

按随机数字表法分为空白对照组、模型对照组、天枢组和大肠俞组

每组10只。采用新生期母子分离、醋酸灌肠和结直肠扩张联合制备IBS模型。9周鼠龄时

天枢组和大肠俞组分别对双侧"天枢"和"大肠俞"进行电针干预

隔日1次

每次20min

共5次。通过改良的腹部回撤反射法评估肠道敏感性和动力

即记录肠道起始收缩波潜伏期、90s内收缩波个数、90s内收缩波峰峰值;采用免疫组化法检测结肠肌层M3受体及黏膜层5-羟色胺(HT)3A受体的阳性表达。结果:与空白对照组相比

模型对照组肠道起始波潜伏期缩短

90s内收缩波个数增多

90s内收缩波峰峰值增大(P<0.01)

M3和5-HT3A受体阳性表达增强(P<0.01)。与模型对照组相比

天枢组和大肠俞组潜伏期延长、峰峰值变小(P<0.01)

天枢组收缩波个数减少(P<0.01)

天枢组和大肠俞组M3和5-HT3A受体阳性表达减少(P<0.01)。与天枢组相比

大肠俞组潜伏期明显缩短(P<0.01)

收缩波个数增多(P<0.05)

M3和5-HT3A受体阳性表达增强(P<0.05

P<0.01)。结论:电针"天枢""大肠俞"穴均能调整IBS大鼠肠道动力和敏感性

且电针"天枢"穴的调节效应优于"大肠俞"

其机制可能与调整结肠内M3受体和5-HT3A受体表达有关。

Abstract

Objective To observe and compare the effect of electroacupuncture(EA)of"Tianshu"(ST25)and"Dachangshu"(BL25)on intestinal sensitivity and the expression of M3 muscarinic acetylcholine receptor(M_(3R))and5-hydroxytryptamine 3 Areceptor(5-HT_(3A)R)in the colon tissue in irritable bowel syndrome(IBS)rats

so as to explore its mechanism underlying improvement of IBS.Methods Forty newborn Wistar rats were randomly divided into blank control

model

ST25 and BL25 groups(n=10 rats per group).The IBS model was established by joint application of maternal separation

acetic acid enema plus colorectal distension from day 8 to 21 after birth.At the age of 9 weeks

EA(2 Hz/100 Hz

0.1-0.3 mA)was applied to ST25 and BL25 for 20 min

once every other day for 5 times.The modified method was used to record the abdominal withdrawal reflex(AWR)

so as to evaluate the visceral sensitivity by referring to the modified Al-Chaer's and colleagues' methods.The threshold(initial wave)

the number and peak-peak value of contraction waves(AWR)within 90 swere recorded.The immunoactivity of M_(3R) and 5-HT_(3A)R in the colon tissue was detected by immunohistochemistry.Results After modeling

the latency was obviously shortened

the number and peak-peak values of contraction waves of intestine were significantly increased

and the immunoactivity of M_(3R) and 5-HT_(3A)R was notably up-regulated in the model group compared with the blank control group(P<0.01).After the EA intervention

the latency was obviously prolonged

the peak-peak value in both ST25 and BL25 groups

and the number of contraction waves in the ST25 group were significantly decreased(P<0.01)

and the expression levels of M3 and 5-HT_(3A)R were considerably down-regulated in both ST25 and BL25 groups relevant to the model group(P<0.01).The therapeutic effects were significantly better in the ST25 group than in the BL25 group in prolonging the latency and reducing the contraction wave number

and in down-regulating the expression of colonic M_(3R) and 5-HT_(3A)R(P<0.05

P<0.01).Conclusion EA at ST25 and BL25 can reduce the intestinal sensitivity in IBS rats

which is probably associated with its effects in down-regulating the expression of M_(3R) and 5-HT_(3A)R in the colon.The therapeutic effects of ST25 were obviously better than those of BL25 in suppressing intestinal sensitivity and expression of colonic M_(3R) and 5-HT_(3A)R.

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