Bone-edge electroacupuncture relieves pain by regulating expression of GRK5, β-arrestin 2 and PKCα proteins in locus coeruleus in bone cancer pain rats with morphine tolerance

DU Jun-ying; CHEN Feng; JIANG Bin; FU Tao-fang; FANG Jian-qiao; LIANG Yi

doi:10.13702/j.1000-0607.190003

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Bone-edge electroacupuncture relieves pain by regulating expression of GRK5, β-arrestin 2 and PKCα proteins in locus coeruleus in bone cancer pain rats with morphine tolerance

更新时间：2023-08-11

- Bone-edge electroacupuncture relieves pain by regulating expression of GRK5, β-arrestin 2 and PKCα proteins in locus coeruleus in bone cancer pain rats with morphine tolerance
- Acupuncture Research Vol. 45, Issue 2, Pages: 87-92(2020)
- 作者机构：
  
  1. 浙江中医药大学第三临床医学院浙江省针灸神经病学研究重点实验室
  2. 浙江中医药大学第二临床医学院
  3. 嘉兴学院附属第一医院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.190003
  CLC：
- Published：2020
- 稿件说明：
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DU Jun-ying, CHEN Feng, JIANG Bin, et al. Bone-edge electroacupuncture relieves pain by regulating expression of GRK5, β-arrestin 2 and PKCα proteins in locus coeruleus in bone cancer pain rats with morphine tolerance[J]. Acupuncture research, 2020, 45(2): 87-92.
DOI：

DU Jun-ying, CHEN Feng, JIANG Bin, et al. Bone-edge electroacupuncture relieves pain by regulating expression of GRK5, β-arrestin 2 and PKCα proteins in locus coeruleus in bone cancer pain rats with morphine tolerance[J]. Acupuncture research, 2020, 45(2): 87-92. DOI： 10.13702/j.1000-0607.190003.

摘要

目的:观察骨边刺电针对骨癌痛吗啡耐受大鼠蓝斑核G蛋白耦联受体激酶5(GRK5)、β-抑制蛋白2(β-arrestin2)、蛋白激酶Cα(PKCα)及其磷酸化(p-PKCα)表达的影响

部分揭示骨边刺电针干预骨癌痛吗啡耐受的中枢机制。方法:SD大鼠随机分为假骨癌痛组、骨癌痛组、吗啡耐受组、骨边刺电针组和假电针组

每组8只。采用胫骨骨髓腔内注射3μL MRMT-1大鼠乳腺癌细胞建立骨癌痛模型

在此基础上通过腹腔多次注射盐酸吗啡注射液(10 mg/kg)建立骨癌痛吗啡耐受模型。骨边刺电针组于成功建立骨癌痛吗啡耐受模型后

开始介入骨边刺电针干预

每日1次

持续7 d。动态观察各组大鼠患侧足跖机械痛阈。采用免疫印迹法观察各组大鼠患侧蓝斑核GRK5、β-arrestin2、PKCα、p-PKCα蛋白表达。结果:与假骨癌痛组比较

癌细胞接种后10 d骨癌痛组大鼠患侧足跖机械痛阈显著降低(P<0.01);吗啡注射后

与骨癌痛组比较

吗啡耐受组、骨边刺电针组和假电针组大鼠患侧足跖机械痛阈显著升高(P<0.01)

而吗啡注射11 d后

吗啡耐受组机械痛阈显著降低

与骨癌痛组比较差异无统计学意义(P>0.05)

出现耐受现象;与吗啡耐受组、假电针组比较

骨边刺电针组干预后机械痛阈明显升高(P<0.01)。与假骨癌痛组比较

吗啡耐受组大鼠蓝斑核GRK5蛋白表达显著降低(P<0.01);与吗啡耐受组和假电针组比较

骨边刺电针组蓝斑核GRK5蛋白表达显著升高(P<0.01)。与假骨癌痛组比较

骨癌痛组大鼠蓝斑核β-arrestin2、p-PKCα蛋白表达显著增多(P<0.01);与吗啡耐受组和假电针组比较

骨边刺电针组蓝斑核β-arrestin2、p-PKCα蛋白表达显著降低(P<0.01)。与吗啡耐受组和假电针组比较

骨边刺电针组蓝斑核PKCα蛋白表达显著降低(P<0.01)。结论:骨边刺电针可有效干预骨癌痛吗啡耐受现象

其机制可能与增加蓝斑核GRK5

抑制β-arrestin2、PKCα及其磷酸化水平有关。

Abstract

Objective To observe the effect of bone-edge electroacupuncture(EA) intervention on mechanical pain threshold(PT) and expression of G protein-coupled receptor kinase(GRK5)

β-arrestin 2

total and phosphorylated PKC alpha(p-PKCα) proteins in the locus coeruleus(LC) of rats with bone cancer pain induced morphine tolerance

so as to reveal its partial central mechanisms underlying pain relief. Methods Forty SD rats were randomly divided into 5 groups

namely sham bone cancer

bone cancer pain

morphine tolerance

bone-edge EA

and sham EA(n= 8 rats in each group). The bone cancer with morphine tolerance model was established by intramedullary injection of MRMT-1 cells into the tibial cavity

and then intraperitoneal injection of morphine hydrochloride injection. After successful establishment of morphine tolerance model

the bone-edge EA(2 Hz/100 Hz

0.5-1.5 mA) was applied to bilateral "Zusanli"(ST36) and "Kunlun"(BL60) for 30 min

once a day for 7 days

after inserting the needle-tip to the tibial bone surface. The ipsilateral mechanical paw withdrawal thresholds(PWTs) were detected dynamically. The expression levels of GRK5

β-arrestin 2

PKCα and p-PKCα in the LC area were measured by Western blot. Results The PWTs of bone cancer pain rats were decreased on day 10 after inoculation of cancer cells(P<0.01). After i.p. of morphine for 11 days

no analgesic effect and pain tolerance appeared(P>0.05). The PWTs were significantly increased in the bone-edge EA intervention group(P<0.01)

not in the sham EA group(P>0.05). In comparison with the sham bone cancer group

the expression of GRK5 protein in morphine tolerance group was significantly decreased(P<0.01); compared with morphine tolerance group

the expression of GRK5 protein in bone-edge EA group was increased(P<0.01). In comparison with the sham bone cancer group

the expression of β-arrestin 2 and p-PKCα in bone cancer group significantly increased(P<0.01). After the intervention

the increased β-arrestin 2 and p-PKCα expressions were reversed in the bone-edge EA group(P<0.01); compared with morphine tolerance group and sham EA group

the expression of PKCα protein was decreased(P<0.01). Conclusion Bone-edge EA can effectively relieve morphine tolerance in bone cancer pain rats

which may be related to its functions in up-regulating GRK5 protein and down-regulating β-arrestin 2

PKCα and p-PKCα proteins in LC.

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