Mechanisms of electroacupuncture underlying treatment of osteoporosis based on HDAC2-mediated osteoblast differentiation pathway

SHAO Yu-wei; SHU Qing; LIU Ruo-lan; WANG Huan-mei; TIAN Jun

doi:10.13702/j.1000-0607.190339

您当前的位置：

首页 >

文章列表页 >

Mechanisms of electroacupuncture underlying treatment of osteoporosis based on HDAC2-mediated osteoblast differentiation pathway

更新时间：2023-08-11

- Mechanisms of electroacupuncture underlying treatment of osteoporosis based on HDAC2-mediated osteoblast differentiation pathway
- Acupuncture Research Vol. 45, Issue 6, Pages: 438-445(2020)
- 作者机构：
  
  1. 武汉大学第二临床学院
  2. 武汉大学中南医院康复医学科
  3. 武汉市第九医院康复科
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.190339
  CLC：
- Published：2020
- 稿件说明：
移动端阅览
SHAO Yu-wei, SHU Qing, LIU Ruo-lan, et al. Mechanisms of electroacupuncture underlying treatment of osteoporosis based on HDAC2-mediated osteoblast differentiation pathway[J]. Acupuncture research, 2020, 45(6): 438-445.
DOI：

SHAO Yu-wei, SHU Qing, LIU Ruo-lan, et al. Mechanisms of electroacupuncture underlying treatment of osteoporosis based on HDAC2-mediated osteoblast differentiation pathway[J]. Acupuncture research, 2020, 45(6): 438-445. DOI： 10.13702/j.1000-0607.190339.

摘要

目的:观察电针对骨质疏松大鼠股骨组蛋白去乙酰化酶2(HDAC2)、组蛋白H3、骨形成相关基因及蛋白表达的影响

探讨电针改善骨质疏松症的机制。方法:将雌性SD大鼠随机分为假手术组、模型组、电针组和阳性药物(E2)组

每组10只。采用去势法建立骨质疏松大鼠模型。电针组电针双侧"肾俞""脾俞"穴

10 min/d;E2组予20μg/mL 17β-雌二醇(E2)皮下注射

100μg/kg。均每周一、三、五治疗

共治疗8周。采用显微计算机断层扫描成像法检测大鼠股骨组织微观结构变化;蛋白质免疫印迹法检测大鼠股骨HDAC2、组蛋白H3和成骨细胞转录因子(Runx2)蛋白表达;比色法及酶联免疫吸附法检测大鼠血清中碱性磷酸酶(ALP)和E2含量;实时荧光定量PCR法检测大鼠股骨Runx2 mRNA表达;免疫荧光双标法检测大鼠股骨中乙酰化组蛋白H3/Runx2及Runx2/ALP蛋白的共表达。结果:与假手术组比较

模型组股骨松质骨密度(TBMD)、骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)、骨小梁数量(Tb.N)均显著降低(P<0.01)

骨小梁分离度(Tb. Sp)、骨小梁模式因子(Tb.Pf)、结构模型指数(SMI)均显著升高(P<0.01)。与模型组比较

电针组大鼠股骨TBMD、BV/TV和Tb. N显著升高(P<0.01

P<0.05)

Tb. Pf和SMI显著下降(P<0.05

P<0.01);E2组大鼠股骨TBMD、BV/TV和Tb. N显著升高(P<0.01)

Tb. Sp、Tb. Pf和SMI均显著下降(P<0.01)。与假手术组比较

模型组大鼠股骨中HDAC2、组蛋白H3表达水平显著升高(P<0.01)

Runx2蛋白和mRNA表达水平显著降低(P<0.01)

血清E2和ALP含量显著降低(P<0.01)

股骨中乙酰化组蛋白H3、Runx2、ALP的荧光强度显著降低(P<0.01)。与模型组比较

电针组HDAC2、组蛋白H3表达水平显著降低(P<0.01)

Runx2蛋白和mRNA表达水平显著升高(P<0.05

P<0.01)

血清ALP含量显著升高(P<0.05)

乙酰化组蛋白H3、Runx2、ALP荧光强度显著升高(P<0.01);E2组Runx2蛋白和mRNA表达水平显著升高(P<0.01)

血清E2和ALP含量显著升高(P<0.01

P<0.05)

Runx2和ALP荧光强度显著升高(P<0.01)。与E2组比较

电针组HDAC2和组蛋白H3蛋白表达水平显著降低(P<0.01)

Runx2 mRNA表达水平显著下降(P<0.05)

乙酰化组蛋白H3荧光强度显著升高、Runx2荧光强度显著降低(P<0.05)。结论:电针治疗骨质疏松可上调体内骨形成相关基因的表达

下调对骨形成通路有抑制作用蛋白的表达

使骨密度升高、骨体积和骨小梁数量增加

并促进骨小梁由杆状向板状变化

其机制可能与组蛋白乙酰化修饰有关。

Abstract

Objective To observe the effect of electroacupuncture(EA) on expression of histone deacetylase 2(HDAC2)

histone H3

bone formation related genes and proteins in osteoporosis rats

so as to reveal its mechanisms underlying improvement of osteoporosis. Methods Female SD rats were randomly divided into 4 groups:sham operation

model

EA and medication(n= 10 rats in each group). The osteoporosis model was established by castration. EA(2 Hz

1 mA) was applied to bilateral "Shenshu"(BL23) and "Pishu"(BL20) for 10 min

once every other day for 8 weeks. Rats of the medication group received subcutaneous injection of 17 β-estradiol(100 μg/kg

20 μg/mL). The bone quality and quantity including the cortical bone mineral density(CBMD)

trabecular bone mineral density(TBMD)

ratio of bone volume/total volume(BV/TV)

trabecular thickness(Tb.Th)

trabecular number(Tb.N)

trabecular separation(Tb. Sp)

trabecular bone pattern factor(Tb.Pf)

and structure model index(SMI) of the right thigh-bone were detected by using a micro-computed tomography. Serum alkaline phosphatase(ALP) and estrogen 2(E2) contents were assayed by using colorimetry and ELISA

expression levels of HDAC2

histone H3 and Runx2 in the thigh-bone were detected using Western blot

and that of Runx2 mRNA was detected using quantitative real-time PCR

separately. The co-expression of Ac-histone H3/Runx2 and Runx2/ALP was observed by using immunofluorescence histochemical staining.Methods After modeling

the levels of TBMD

BV/TV

Tb.Th

and Tb.N

serum E2 and ALP

and expression of Runx2 protein and mRNA

Ac-histone and ALP proteins were significantly lower(P<0.01)

and those of Tb.Sp

Tb.Pf and SMI

HDAC2 and histone H3 proteins were significantly higher(P<0.01) in the model group than those in the sham operation group. After the interventions

the decrease of TBMD

BV/TV

Tb.N

Runx2 protein and mRNA

ALP in both EA and medication groups

serum E2 in the medication group

and Ac-histone H3 in the EA group

and the increase of Tb.Sp in the medication group

Tb.Pf

SMI

and HDAC2 in both EA and medication groups

and histone H3 in the EA group were reversed(P<0.01

P<0.05). No significant changes were found in the levels of CBMD after modeling relevant to the sham operation group

and after EA and medication interventions(P>0.05). The effects of EA were significantly superior to 17 β-estradiol in down-regulating the expression of HDAC2 and histone H3 proteins and in up-regulating expression of Ac-histone H3 protein(P<0.01

P<0.05).Conclusion EA treatment can increase bone density

increase bone mass and trabecular bone

and promote trabecular bone rod-like changes in plate shape in osteoporosis rats

which is related to its effect in up-regulating the expression of Ac-histone H3 protein

and down-regulating the expression of bone formation-related proteins.

关键词

Keywords

references

Views

311

下载量

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Electroacupuncture of “Jiaji”（EX-B2） improves asthma by reducing airway inflammation and regulating PI3K/AKT/mTOR signaling pathway in rats with allergic asthma

Effect of electroacupuncture on expression in blood-brain barrier in rats with cerebral ischemia-reperfusion injury by regulating HIF-1α/VEGF/MMP-9 signaling pathway

Research progress on the mechanism of acupuncture based on microbiota-gut-brain axis

Exploration of the mechanism of electroacupuncture at “Jiaji” （EX-B2） in treating neuronal apoptosis in rats with spinal cord injury based on the JAK2/STAT3 pathway

Study on the underlying autonomic nervous mechanism of electroacupuncture in improving colonic inflammation in mice with chronic ulcerative colitis

Related Author

ZHU Tao

LI Jie

WAN Hong-ye

CHANG Bu

CHENG Yan-ting

JI Yu-fang

JI Lai-xi

LIAO Teng-wei

Related Institution

College of Acupuncture-Moxibustion and Tuina， Chengdu University of Traditional Chinese Medicine

The Second Clinical Medicine College， Shanxi University of Chinese Medicine

Institute of Acupuncture and Moxibustion， China Academy of Chinese Medical Sciences

Clinical Medical College of Acupuncture-Moxibustion and Rehabilitation， Guangzhou University of Chinese Medicine

The Second Clinical College， Guangzhou University of Chinese Medicine

Postal code：100700
Tel：010-64089344,84014607 Email：zcyjbjb@vip.163.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05017590号-8 京公网安备11010802020460号
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰