Correlation between blood oxygen level dependent fMRI signal and GABA content in anterior cingulate cortex after acupuncture of Hegu(LI4)

SUN Li; CHEN Yuan-yuan; FANG Ji-liang; HONG Yang; WANG Yin; XU Ke; LI Xiao-jiao; CHEN Li-mei; ZHANG Guo-lei; ZHAO Yan-ping

doi:10.13702/j.1000-0607.190597

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Correlation between blood oxygen level dependent fMRI signal and GABA content in anterior cingulate cortex after acupuncture of Hegu(LI4)

更新时间：2023-08-10

- Correlation between blood oxygen level dependent fMRI signal and GABA content in anterior cingulate cortex after acupuncture of Hegu(LI4)
- Acupuncture Research Vol. 44, Issue 12, Pages: 878-883(2019)
- 作者机构：
  
  1. 中国中医科学院广安门医院放射科
  2. 中国中医科学院广安门医院针灸科
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.190597
  CLC：
- Published：2019
- 稿件说明：
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SUN Li, CHEN Yuan-yuan, FANG Ji-liang, et al. Correlation between blood oxygen level dependent fMRI signal and GABA content in anterior cingulate cortex after acupuncture of Hegu(LI4)[J]. Acupuncture research, 2019, 44(12): 878-883.
DOI：

SUN Li, CHEN Yuan-yuan, FANG Ji-liang, et al. Correlation between blood oxygen level dependent fMRI signal and GABA content in anterior cingulate cortex after acupuncture of Hegu(LI4)[J]. Acupuncture research, 2019, 44(12): 878-883. DOI： 10.13702/j.1000-0607.190597.

摘要

目的:"面口合谷收"的大脑机制尚未明确

本研究通过观察针刺右侧合谷穴时前额叶脑皮层血流的血氧水平依赖功能磁共振成像(BOLD-fMRI)信号与相应皮层内磁共振波谱(MRS)检测到的神经递质γ-氨基丁酸(GABA)变化的关系

探索针刺合谷穴的作用机制。方法:本研究共招募到76名青年健康受试者

男23名

女53名

平均年龄(24. 5±1. 4)岁。受试者接受两次fMRI-MRS扫描

手针针刺和Von Frey纤毛针表皮刺激右侧合谷穴

两种刺激方式顺序随机分配

两次间隔1周。扫描序列含任务前静息态BOLD、MRS和任务态BOLD、MRS。感兴趣区(ROI)定位于双侧前额叶内侧皮层。fMRI数据采用SPM12、REST软件进行处理

MRS数据采用LC Model软件处理。随后进行脑激活区的组内、组间比较

计算ROI内皮层BOLD信号定量值

测量ROI内GABA值并进行矫正。最后统计分析BOLD信号与GABA含量的关系。结果:手针针刺与Von Frey纤毛针表皮刺激均诱发了大脑皮层广泛的BOLD负激活信号

主要集中在中线区(包含前额叶内侧皮层)和边缘叶脑区

但Von Frey纤毛针表皮刺激诱发的信号较少而弱。两组前额叶内侧皮层ROI内的BOLD信号与GABA+值在基线、任务后及其前后的差值均未发现明显相关性(手针针刺组:r=-0. 07

-0. 08

0. 04;P=0. 57

0. 88

0. 74;Von Frey纤毛针表皮刺激组:r=-0. 10

-0. 09

-0. 01;P=0. 43

0. 46

0. 96)。结论:手针针刺较Von Frey纤毛针表皮刺激合谷穴激发了更强更广的包含前额叶内侧皮层的边缘叶-旁边缘叶-新皮层网络负激活效应

但两种刺激下的前额叶内侧皮层ROI区域内BOLD信号与GABA含量及其变化未发现明确相关性

需进一步扩大样本量及在疾病状态下研究证实。

Abstract

ObjectiveTo explore the correlation between blood oxygen level dependent functional magnetic resonance imaging(BOLD-fMRI)signal and neurotransmitter γ-aminobutyric acid(GABA)concentration in the prefrontal cortex area after acupuncture or Von Frey filament stimulation(epidermal stimulation)at the right Hegu(LI4).MethodsA total of 76 healthy volunteers(23 men and 53 women

24. 5±1. 4 years in age)were recruited in the present study. Each volunteer received two sessions of fMRI magnetic resonance scanning(MRS)examinations

with an interval of one week between two sessions. The MRI scan sequences included pre-task MRS

resting state BOLD and task MRS

BOLD. A region of Interest(ROI)of 35 mm×30 mm×25 mm was located at the bilateral medial prefrontal cortex areas. In the two sessions of examinations

the right LI4 point was stimulated by manual acupuncture or Von Frey filament-pressing. The tasks were designed as the block design. Each block contained 3 intermittent acupoint stimulations

lasting 30 s in each stimulation and with two minutes' pause between two stimulations. The MRS data were processed by using Linear Combination(LC)Model software(for assessing GABA content)

and the BOLD data of fMRI was analyzed by using SPM12 software(comparison within each group)

REST1. 8(comparison between two groups)

separately.ResultsExtensive deactivations were induced by both stimulations

mainly involving the midline regions as the medial prefrontal lobe

and limbic lobe. The deactivation effect of manual acupuncture stimulation was more extensive and intensive than that of Von Frey filament stimulation

especially in the medial prefrontal lobe. Data from 66 volunteers(after exclusion of 10 participants due to bigger standard deviation of GABA/Glx)showed no marked correlation between the GABA concentration and BOLD activation in the anterior cingulate cortex area in both groups(manual acupuncture stimulation group:r=-0. 07

-0. 08

0. 04;P=0. 57

0. 88

0. 74;Von Frey filament epidermal stimulation group:r=-0. 10

-0. 09

-0. 01;P=0. 43

0. 46

0. 96).ConclusionAcupuncture of LI4 elicits a stronger and broader negative activation effect in the limbic-paralimbic-neocortical network including the medial prefrontal cortex in comparison with Von Frey filament stimulation

but no apparent correlation was found between the GABA concentration and BOLD activation in the anterior cingulate cortex after manual acupuncture and Von Frey stimulation.

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Related Institution

School of Rehabilitation Medicine， Henan University of Chinese Medicine

Rehabilitation Center， the First Affiliated Hospital of Henan University of Chinese Medicine

Department of Traditional Chinese Medicine, Fujian Medical University Union Hospital

Department of Traditional Chinese Medicine, The First Affiliated Hospital of Fujian Medical University

Department of Acupuncture and Moxibustion, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine

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