Effect of electroacupuncture of “Hegu” (LI4) and “Zusanli” (ST36) on intestinal sensitivity and motility in irritable bowel syndrome rats

QIN Ying; GUO Meng-wei; LAN Ying; WANG Yi-fan; WANG Shan; JI Mao-xian; REN Xiao-xuan; ZHU Wen-lian

doi:10.13702/j.1000-0607.190743

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Effect of electroacupuncture of “Hegu” (LI4) and “Zusanli” (ST36) on intestinal sensitivity and motility in irritable bowel syndrome rats

更新时间：2023-08-11

- Effect of electroacupuncture of “Hegu” (LI4) and “Zusanli” (ST36) on intestinal sensitivity and motility in irritable bowel syndrome rats
- Acupuncture Research Vol. 45, Issue 4, Pages: 293-298(2020)
- 作者机构：
  
  北京中医药大学针灸推拿学院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.190743
  CLC：
- Published：2020
- 稿件说明：
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QIN Ying, GUO Meng-wei, LAN Ying, et al. Effect of electroacupuncture of “Hegu” (LI4) and “Zusanli” (ST36) on intestinal sensitivity and motility in irritable bowel syndrome rats[J]. Acupuncture research, 2020, 45(4): 293-298.
DOI：

QIN Ying, GUO Meng-wei, LAN Ying, et al. Effect of electroacupuncture of “Hegu” (LI4) and “Zusanli” (ST36) on intestinal sensitivity and motility in irritable bowel syndrome rats[J]. Acupuncture research, 2020, 45(4): 293-298. DOI： 10.13702/j.1000-0607.190743.

摘要

目的:比较电针"合谷"和"足三里"穴对肠易激综合征(IBS)大鼠肠道敏感性和动力的影响

探讨其效应差异及相关机制。方法:新生Wistar大鼠40只

随机分为空白对照组、模型对照组、合谷组和足三里组

每组10只。采用母婴分离、醋酸灌肠结合结直肠扩张的方法制备IBS模型。两穴位组从第9周开始给予电针治疗

隔日1次

共5次。通过Bristol便型分类法及改良的腹部回撤反射(AWR)法评估肠道动力和敏感性;采用免疫组织化学法检测结肠黏膜层及肌层5-羟色胺3A受体(5-HT_(3A)R)的阳性表达。结果:造模后

3个造模组的Bristol评分均明显高于空白对照组(P<0.01)。与空白对照组比较

模型对照组的Bristol评分明显上升(P<0.01)

AWR潜伏期明显缩短、收缩波个数明显增多(P<0.01)

结肠黏膜层和肌层5-HT_(3A)R表达均明显升高(P<0.01);与模型对照组比较

两穴位组的Bristol评分明显降低(P<0.01)

AWR潜伏期明显延长、收缩波个数明显减少(P<0.01

P<0.05)

结肠黏膜层和肌层5-HT_(3A)R表达明显降低(P<0.01);与合谷组比较

足三里组的Bristol评分明显降低(P<0.01)

AWR潜伏期明显缩短、收缩波个数明显增多(P<0.05)

结肠黏膜层5-HT_(3A)R表达明显升高(P<0.01)

肌层5-HT_(3A)R表达明显降低(P<0.01)。结论:电针"合谷""足三里"穴能有效调节IBS模型大鼠肠道高敏感性和动力异常

改善腹痛和腹泻的症状

且"合谷"穴治疗腹痛即肠道敏感性的效果更佳

而"足三里"穴治疗腹泻即肠道动力的效果更好

说明穴位存在效应特异性。

Abstract

Objective To observe the effect of electroacupuncture(EA) of "Hegu"(LI4) and "Zusanli"(ST36)on changes of intestinal sensitivity and colonic motility and expression of colonic 5-hydroxytryptamine 3 A receptor(5-HT_(3 A)R) in irritable bowel syndrome(IBS) rats

so as to reveal its mechanism underlying improvement of IBS. Methods A total of 40 neonatal Wistar rats were randomly and equally divided into normal control

model

LI4 and ST36 groups(n=10). The IBS model was induced by mother-infant separation

acetic acid enema and colorectal distension(CRD). EA(2 Hz/100 Hz

a tolerable strength) was applied to bilateral LI4 and ST36 for 20 min

once every other day for 5 times. The Bristol stool form scale was used to assess the gastrointestinal function

and the latency and number of abdominal muscular contraction waves of abdominal withdrawal reflex(AWR) were used to evaluate the intestinal sensitivity and motility respectively. The immunoactivity of 5-HT_(3 A)R of the colon tissue was detected by immunohistochemistry. Results After modeling

the score of Bristol fecal form scale

number of muscular contraction waves and expression levels of colonic 5-HT_(3 A)R in the myometrium and mucosal layers were significantly increased(P<0.01)

and the latency of muscular initial contraction wave was obviously shortened in the model group relevant to the normal control group(P<0.01). After the intervention

the increased Bristol fecal form score

number of muscular contraction waves and expression levels of 5-HT_(3 A)R in the myometrium and mucosal layers as well as the decreased latency of muscular contraction were reversed in both LI4 and ST36 groups(P<0.01

P<0.05). The effect of EA of ST36 was significantly superior to those of EA-LI4 in lowering Bristol fecal scale score and 5-HT_(3 A)R expression in the muscular layer(P<0.01)

but obviously inferior to those of EA-LI4 in increasing the latency of of muscular initial contraction wave and down-regulating muscular contraction waves and 5-HT_(3 A)R expression in the mucosal layer(P<0.05

P<0.01). Conclusion Both EA-LI4 and EA-ST36 can significantly improve the symptoms of abdominal pain and diarrhea

but EA-LI4 is better in suppressing intestinal high sensitivity

and EA-ST36 is better in promoting intestinal motility

suggesting a specificity of effect of acupoints of different meridians.

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Related Institution

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