Moxibustion combined with acupoint catgut embedding promotes recovery of injured colonic mucosa by suppressing inflammation in ulcerative colitis rats

YU Qian-hui; LI Li-hong; LU Yu-wei; HOU Xun-rui; LI Lin; WANG Yu-jia; KONG Ting-ting; LIANG Xin

doi:10.13702/j.1000-0607.190772

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Moxibustion combined with acupoint catgut embedding promotes recovery of injured colonic mucosa by suppressing inflammation in ulcerative colitis rats

更新时间：2023-08-11

- Moxibustion combined with acupoint catgut embedding promotes recovery of injured colonic mucosa by suppressing inflammation in ulcerative colitis rats
- Acupuncture Research Vol. 45, Issue 4, Pages: 305-309(2020)
- 作者机构：
  
  1. 贵州医科大学临床医学院
  2. 贵州医科大学附属医院
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.190772
  CLC：
- Published：2020
- 稿件说明：
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YU Qian-hui, LI Li-hong, LU Yu-wei, et al. Moxibustion combined with acupoint catgut embedding promotes recovery of injured colonic mucosa by suppressing inflammation in ulcerative colitis rats[J]. Acupuncture research, 2020, 45(4): 305-309.
DOI：

YU Qian-hui, LI Li-hong, LU Yu-wei, et al. Moxibustion combined with acupoint catgut embedding promotes recovery of injured colonic mucosa by suppressing inflammation in ulcerative colitis rats[J]. Acupuncture research, 2020, 45(4): 305-309. DOI： 10.13702/j.1000-0607.190772.

摘要

目的:观察穴位埋线联合艾灸对溃疡性结肠炎(UC)大鼠体质量、大便性状、出血情况、肠黏膜组织形态及白细胞介素-6(IL-6)表达的影响

探讨艾灸和埋线联合应用对UC大鼠的抗炎修复作用。方法:将SD大鼠随机分为正常组、模型组、艾灸组、埋线组、埋线加艾灸组

每组6只。采用三硝基苯磺酸联合乙醇灌肠法复制UC大鼠模型。穴位选取双侧"天枢""大肠俞""上巨虚"。艾灸组给予温和灸

每次10 min

每日1次

共干预14次。埋线组给予穴位简易埋线

每周1次

共干预2次。埋线加艾灸组干预方法同艾灸组、埋线组

埋线干预于温和灸干预6 h后进行。观察各组大鼠一般情况、体质量、大便性状、出血情况

并进行疾病活动指数(DAI)评估;HE染色法观察结肠黏膜组织形态变化;免疫组织化学法和Western blot法检测结肠黏膜IL-6的表达。结果:与正常组比较

模型组大鼠饮食减少

活动、精神差

易激惹

毛发少泽

便稀或不成形

肛周红肿伴脓血分泌;治疗后3个治疗组一般情况较模型组均有不同程度的改善。与正常组比较

模型组大鼠DAI明显升高(P<0.01);与模型组比较

埋线加艾灸组、埋线组、艾灸组DAI均明显降低(P<0.01

P<0.05);埋线加艾灸组DAI明显低于埋线组、艾灸组(P<0.01)。HE染色结果显示

正常组结肠组织结构完整清晰;模型组结肠黏膜上层缺损严重

有大量炎性细胞浸润;各治疗组大鼠结肠组织损伤均有所改善

有少量炎性细胞浸润

其中埋线加艾灸组改善最明显。与正常组比较

模型组结肠黏膜组织IL-6阳性表达及蛋白表达量明显增高(P<0.01);与模型组比较

埋线加艾灸组、埋线组、艾灸组结肠黏膜组织IL-6阳性表达及蛋白表达量明显降低(P<0.01

P<0.05);且埋线加艾灸组IL-6明显低于埋线组、艾灸组(P<0.01)。结论:埋线联合艾灸能有效降低UC大鼠DAI

改善结肠黏膜的病理损伤

下调促炎因子IL-6的表达

从而达到对UC大鼠结肠黏膜的抗炎修复作用。

Abstract

Objective To observe the effect of moxibustion and acupoint catgut embedding(ACE) at "Tianshu"(ST25) "Dachangshu"(BL25) and "Shangjuxu"(ST37) on changes of body mass

stool property

histopathological conditions and expression levels of interleukin 6(IL-6) in colonic mucosa of ulcerative colitis(UC) rats

so as to reveal its anti-inflammatory mechanisms underlying improvement of UC. Methods SD rats were randomized into normal

model

moxibustion

ACE and moxibustion+ACE groups(n=6 in each group). The UC model was established by enema of trinitro-benzene-sulfonic acid and ethanol. Moxibustion was applied to bilateral ST25

BL25 and ST37 for 10 min

once daily for 14 days

and ACE applied to the same 3 acupoints

once a week for two weeks. After the treatment

the rats' general conditions were observed

and the severity of UC was assessed by using disease activity index(DAI) score. Colonic mucosal pathological changes were observed under microscope after hematoxylin eosin(H.E.) stain

and the expression levels of IL-6 in the colonic mucosa tissue detected by using immunohistochemical stain and Western blot

respectively. Results After modeling

the DAI score

and expression level of colonic IL-6 protein detected by immunohistochemistry and Western blot were obviously increased in the model group relevant to the normal group(P<0.01). Following the intervention

the increase of DAI score and IL-6 expression were reversed in moxibustion

ACE and moxibustion+ACE groups(P<0.01

P<0.05). The therapeutic effects of moxibustion+ACE were considerably superior to those of simple ACE and simple moxibustion in down-regulating the levels of DAI score and IL-6 expression(P<0.01). H.E. staining showed severe defect of the colonic mucosal epithelium with infiltration of a large number of inflammatory cells in the model group

which was milder in moxibustion

ACE and moxibustion+ACE groups. Conclusion Moxibustion combined with ACE is able to improve the inflammatory injury of colonic mucosa in UC rats

which may be related with its effect in suppressing the expression of colonic IL-6; and the efficacy of moxibustion+ACE is apparently superior to that of moxibustion and ACE alone.

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Related Author

WANG Yu-jia

YU Qian-hui

LU Yu-wei

ZHANG Qian

HOU Xun-rui

ZHOU Yu-ling

LI Li-hong

XIE Chao-qun

Related Institution

College of Acu-moxibustion and Tuina, Changchun University of Chinese Medicine

Affiliated Hospital of Guizhou Medical University

Department of Spleen and Stomach Disease, The First Affiliated Hospital of Hunan University of Chinese Medicine

Yueyang hospital affiliated to Hunan University of Chinese Medicine

The First Affiliated Hospital of Hunan University of Chinese Medicine

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