Moxibustion relieves abdominal hypersensitivity and diarrhea by regulating colonic 5-hydroxytryptamine signaling pathway in rats with diarrhea type irritable bowel syndrome

TONG Li; WU Li-bin; LI Nan; CHENG Hong-liang; CAI Rong-lin; CHU Hao-ran

doi:10.13702/j.1000-0607.190857

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Moxibustion relieves abdominal hypersensitivity and diarrhea by regulating colonic 5-hydroxytryptamine signaling pathway in rats with diarrhea type irritable bowel syndrome

更新时间：2023-08-11

- Moxibustion relieves abdominal hypersensitivity and diarrhea by regulating colonic 5-hydroxytryptamine signaling pathway in rats with diarrhea type irritable bowel syndrome
- Acupuncture Research Vol. 45, Issue 7, Pages: 535-540(2020)
- 作者机构：
  
  1. 安徽中医药大学研究生院
  2. 安徽中医药大学第二附属医院
  3. 安徽中医药大学针灸经络研究所
  4. 安徽省中医药科学院针灸临床研究所
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.190857
  CLC：
- Published：2020
- 稿件说明：
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TONG Li, WU Li-bin, LI Nan, et al. Moxibustion relieves abdominal hypersensitivity and diarrhea by regulating colonic 5-hydroxytryptamine signaling pathway in rats with diarrhea type irritable bowel syndrome[J]. Acupuncture research, 2020, 45(7): 535-540.
DOI：

TONG Li, WU Li-bin, LI Nan, et al. Moxibustion relieves abdominal hypersensitivity and diarrhea by regulating colonic 5-hydroxytryptamine signaling pathway in rats with diarrhea type irritable bowel syndrome[J]. Acupuncture research, 2020, 45(7): 535-540. DOI： 10.13702/j.1000-0607.190857.

摘要

目的:观察艾灸对腹泻型肠易激综合征(IBS-D)模型大鼠内脏敏感性、稀便率、结肠组织中5-羟色胺(5-HT)信号通路相关分子表达的影响

探讨艾灸治疗IBS-D的机制。方法:健康雄性SD大鼠随机分为空白组、模型组和艾灸组

每组10只。采用慢性束缚联合番泻叶灌胃法建立IBS-D大鼠模型。艾灸组艾灸大鼠"天枢""上巨虚"

每天1次

每次30 min

共干预7 d。干预结束后

观察各组大鼠稀便率和直肠扩张所引起腹部回缩反射的最小容量阈值

酶联免疫吸附法检测结肠中5-HT含量

荧光定量PCR法检测结肠组织中色氨酸羟化酶1(TPH1)、5-HT转运体(SERT)和5-羟色胺受体3(5-HT3R)的mRNA表达量

Western blot法检测结肠组织中TPH1、SERT和5-HT3R的蛋白表达水平。结果:与空白组比较

模型组腹部回缩反射的最小容量阈值明显下降(P<0.01)

稀便率、结肠中5-HT含量及TPH1、5-HT3R的蛋白和mRNA表达量明显升高(P<0.01)

SERT的蛋白和mRNA表达量明显降低(P<0.01)。与模型组比较

艾灸组腹部回缩反射的最小容量阈值明显升高(P<0.01)

稀便率、结肠中5-HT含量及结肠组织中TPH1、5-HT3R的蛋白和mRNA表达量均明显降低(P<0.01)

SERT的蛋白和mRNA表达量明显升高(P<0.01)。结论:艾灸"天枢""上巨虚"可能是通过对IBS-D大鼠结肠组织中5-HT信号通路的多靶点协同作用来治疗IBS-D。

Abstract

Objective To observe the effect of moxibustion on visceral pain

diarrhea

colonic 5-hydroxytryptamine(5-HT) content

and expression of tryptophan hydroxylase 1(TPH1)

serotonin reup take transporter(SERT) and 5-hydroxytryptamine receptor 3(5-HT3 R) in colon tissue of rats with diarrhea irritable bowel syndrome(IBS-D)

so as to reveal its underlying mechanisms in treating IBS-D. Methods Thirty male SD rats were randomly divided into blank control

model and moxibustion groups(n=10 rats in each group). The IBS-D model was established by chronic restraint combined with gavage of Senna leaf solution. Moxibustion was applied to bilateral "Tianshu"(ST25) and "Shangjuxu"(ST37) for 30 min

once a day for 7 days. After the treatment

the loose stool rate(number of loose stool/total number of feces granules X100%) and the minimum volume threshold of abdominal reflex(abdominal pain threshold) induced by rectal dilatation were observed. The content of colonic 5-HT was detected by using ELISA

and the expression of TPH1

SERT and 5-HT3 R mRNAs and proteins were detected by using quantitative real time-PCR and Western blot

respectively. Results Compared with the blank control group

the minimum volume threshold of abdominal retraction reflex and the relative expression of SERT protein and mRNA were significantly decreased(P<0.01)

and the loose stool rate

colonic 5-HT content

and relative expression of TPH1 and 5-HT3 R proteins and mRNAs were notably increased in the model group(P<0.01). After moxibustion

both the decrease of minimum volume threshold and SERT protein and mRNA expressions and the increase of loose stool rate

colonic 5-HT content and TPH1 and 5-HT3 R protein and mRNA expressions were reversed(P<0.01). Conclusion Moxibustion of ST25 and ST37 can relieve abdominal hypersensitivity and diarrhea in IBS-D model rats

which is related to its effects in down-regulating colonic 5-HT content and expression of TPH1 and 5-HT3 R proteins and mRNAs and in up-regulating expression of SERT protein and mRNA(regulating 5-HT/5-HT3 R signaling).

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