Electroacupuncture at “Ganshu” (BL18) and “Yanglingquan” (GB34) alleviates hepatic ischemia-reperfusion injury by inhibiting translocation and release of high mobility group protein 1 in rats

TAN Si-you; HUANG Jun; SU Ying-ying; CHEN Wen-yan; KONG Gao-yin; LIU Jing-shi; WEI Lai

doi:10.13702/j.1000-0607.200374

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Electroacupuncture at “Ganshu” (BL18) and “Yanglingquan” (GB34) alleviates hepatic ischemia-reperfusion injury by inhibiting translocation and release of high mobility group protein 1 in rats

更新时间：2023-08-10

- Electroacupuncture at “Ganshu” (BL18) and “Yanglingquan” (GB34) alleviates hepatic ischemia-reperfusion injury by inhibiting translocation and release of high mobility group protein 1 in rats
- Acupuncture Research Vol. 45, Issue 11, Pages: 888-894(2020)
- 作者机构：
  
  1. 湖南师范大学附属第一医院/湖南省人民医院麻醉科
  2. 湖南省围术期加速康复麻醉临床医学研究中心
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.200374
  CLC：
- Published：2020
- 稿件说明：
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TAN Si-you, HUANG Jun, SU Ying-ying, et al. Electroacupuncture at “Ganshu” (BL18) and “Yanglingquan” (GB34) alleviates hepatic ischemia-reperfusion injury by inhibiting translocation and release of high mobility group protein 1 in rats[J]. Acupuncture research, 2020, 45(11): 888-894.
DOI：

TAN Si-you, HUANG Jun, SU Ying-ying, et al. Electroacupuncture at “Ganshu” (BL18) and “Yanglingquan” (GB34) alleviates hepatic ischemia-reperfusion injury by inhibiting translocation and release of high mobility group protein 1 in rats[J]. Acupuncture research, 2020, 45(11): 888-894. DOI： 10.13702/j.1000-0607.200374.

摘要

目的:研究电针"肝俞""阳陵泉"对大鼠肝脏缺血再灌注损伤(HIRI)的保护作用和对肝脏组织高迁移率族蛋白1(HMGB1)表达的影响。方法:雄性SD大鼠随机分为假手术组、模型组、肝俞-阳陵泉组和非经非穴组

每组10只。采用无创动脉夹阻断供应肝中叶和左叶的动脉、静脉和胆管1 h

解除阻断后再灌注4 h以构建70%HIRI模型。肝俞-阳陵泉组取两侧"肝俞"和"阳陵泉"

非经非穴组取两侧"肝俞"穴区旁开6～8 mm的非经非穴区肌肉结缔组织

此二组均在造模前给予电针刺激30 min。以HE染色法观察肝脏组织损伤情况

全自动生化分析仪检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)水平

酶联免疫法检测血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和HMGB1水平

免疫组织化学法和蛋白质免疫印迹法检测肝脏组织HMGB1蛋白表达

荧光定量PCR检测肝脏组织HMGB1 mRNA表达。结果:与假手术组比较

造模后大鼠肝脏损伤评分及血清ALT、AST、TNF-α、IL-6和HMGB1含量均显著升高(P<0.01)

肝脏组织中的HMGB1阳性细胞数、HMGB1 mRNA及蛋白表达均明显升高(P<0.01)。与模型组比较

肝俞-阳陵泉组大鼠肝脏损伤评分及血清ALT、AST、TNF-α、IL-6和HMGB1含量均降低(P<0.05)

肝脏组织中的HMGB1阳性细胞数、HMGB1 mRNA及蛋白表达降低(P<0.05)。非经非穴组大鼠肝脏损伤评分及血清ALT、AST、TNF-α、IL-6和HMGB1含量均高于肝俞-阳陵泉组(P<0.05)

肝脏组织中HMGB1阳性细胞数、HMGB1 mRNA及蛋白表达均高于肝俞-阳陵泉组(P<0.05)。结论:电针"肝俞""阳陵泉"可减轻大鼠肝脏缺血再灌注损伤

这种保护作用可能是通过抑制HMGB1从胞核向胞质移位和释放、下调相关炎性因子表达来发挥的。

Abstract

Objective To explore the protective effect of electroacupuncture(EA) on hepatic ischemia-reperfusion injury(HIRI) and the expression of high mobility group protein 1(HMGB1) in liver tissues in rats. Methods A total of 40 male SD rats were randomly divided into 4 groups

namely sham control

HIRI model

"Ganshu"(BL18)-"Yanglingquan"(GB34) and non-acupoint group

with 10 rats in each group. The HIRI model was induced by blocking the arteries

veins and bile ducts supplying the middle and left lobes of the liver for 1 h

and reperfusion for 4 h to induce an area of about 70% HIRI. EA was applied to bila-teral BL18 and GB34

or non-acupoints about 6—8 mm to the bilateral BL18 for 30 min before modeling. Serum alanine transaminase(ALT) and aspartate aminotransferase(AST) levels were measured by using an automatic biochemical analyzer. Serum tumor necrosis factor-α(TNF-α)

interleukin-6(IL-6) and HMGB1 levels were assayed by ELISA. Hematoxylin-eosin(H.E.) staining was used to observe histopathological changes of the liver tissue by using tissue injury scaling(0-3 scores). The expression of HMGB1 protein in liver tissues was detected by immunohistochemical staining

Western blot and PCR

separately. Results Following modeling and compared with the sham group

the levels of serum ALT

AST

TNF-α

IL-6

and HMGB1 contents

the number of HMGB1 immunoreaction(IR)-positive cells

and HMGB1 protein and mRNA were significantly increased(P<0.01). After the treatment

the contents of serum ALT

AST

TNF-α

IL-6

and HMGB1

liver HMGB1 IR-positive cells

protein and mRNA were considerably down-regulated in the BL18-GB34 group(P<0.05)

rather than in the non-acupoint group(P>0.05) in contrast to the model group. H.E. stain showed a higher liver injury score in the model group than in the sham group(P<0.01)

and a lower liver injury score in the BL18-GB34 group(not the non-acupoint group) relevant to the model group(P<0.05). Conclusion EA of BL18 and GB34 points has a protective effect on ischemic liver injury in rats with HIRI

which may be associated with its functions in inhibiting the migration and release of HMGB1 from the nucleus to the cytoplasm and in down-regulating the expression of inflammatory factors.

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