Effect of combined administration of electroacupuncture and mesenchymal stem cells on expression of endometrium estrogen receptor and progesterone receptor in thin endometrium rats

MENG Qing-yu; XI Jin; XIA Liang-jun; YANG Bin; CHENG Jie; XIA You-bing; Wan Qian

doi:10.13702/j.1000-0607.200971

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Effect of combined administration of electroacupuncture and mesenchymal stem cells on expression of endometrium estrogen receptor and progesterone receptor in thin endometrium rats

更新时间：2023-08-11

- Effect of combined administration of electroacupuncture and mesenchymal stem cells on expression of endometrium estrogen receptor and progesterone receptor in thin endometrium rats
- Acupuncture Research Vol. 46, Issue 5, Pages: 385-390(2021)
- 作者机构：
  
  1. 南京中医药大学针灸推拿·养生康复学院
  2. 南京中医药大学医学院·整合医学院
  3. 徐州医科大学
  4. 南京大学医学院附属鼓楼医院中医科
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.200971
  CLC：
- Published：2021
- 稿件说明：
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MENG Qing-yu, XI Jin, XIA Liang-jun, et al. Effect of combined administration of electroacupuncture and mesenchymal stem cells on expression of endometrium estrogen receptor and progesterone receptor in thin endometrium rats[J]. Acupuncture research, 2021, 46(5): 385-390.
DOI：

MENG Qing-yu, XI Jin, XIA Liang-jun, et al. Effect of combined administration of electroacupuncture and mesenchymal stem cells on expression of endometrium estrogen receptor and progesterone receptor in thin endometrium rats[J]. Acupuncture research, 2021, 46(5): 385-390. DOI： 10.13702/j.1000-0607.200971.

摘要

目的:观察电针联合骨髓间充质干细胞注射对大鼠薄型子宫内膜雌、孕激素受体的影响。方法:SD大鼠分为空白组、模型组、细胞组、联合组

每组10只。用95%乙醇宫腔注射法复制薄型子宫内膜大鼠模型。细胞组与联合组于造模当日、造模后第3天、第2个动情期、第3个动情期当日由尾静脉进行干细胞悬液注射。联合组除干细胞注射外

予电针"关元""子宫""三阴交"

每日1次

连续10 d。3个动情周期后的动情期取子宫标本

计算子宫系数

免疫组织化学法检测子宫组织KI67表达

Western blot法检测子宫组织雌激素受体、孕激素受体的表达。结果:与空白组比较

模型组子宫系数及雌激素受体、孕激素受体表达量均显著降低(P<0.001

P<0.01)。与模型组比较

细胞组、联合组子宫系数及KI67、雌激素受体、孕激素受体表达量均明显升高(P<0.000 1

P<0.001

P<0.01)。联合组孕激素表达量比细胞组升高(P<0.05)。结论:电针联合骨髓间充质干细胞注射可促进大鼠薄型子宫内膜细胞增殖

升高雌、孕激素受体水平。

Abstract

Objective To investigate the effect of joint administration of electroacupuncture(EA) and bone marrow mesenchymal stem cells(BMMSC) on the expression of estrogen receptor(ER) and progesterone receptor(PR) in thin endometrium rats

so as to explore its mechanisms underlying improvement of conception rate. Methods Forty female SD rats were divided into control

model

BMMSC and EA+BMMSC groups. The thin endometrium model was established by intrauterine infusion of 95% ethanol and saline. EA(1 mA

2 Hz/15 Hz) was applied to "Guanyuan"(CV4)

unilateral "Zigong"(EX-CA1) and unila-teral "Sanyinjiao"(SP6) for 15 min

once daily for 10 d. Rats of the BMMSC and EA+BMMSC groups received injection of BMMSC suspension through caudal vein on the day of modeling

the 3

(rd)

day after surgery

and the 2(rd) day after surgery

and the 2

(nd)

and the 3(nd) and the 3

(rd)

estrus phases

respectively. Changes of body weight and estrus phase were continuously recorded. After three estrus cycles

uterine specimens were taken and the uterine coefficient was calculated. The immunoactivity of KI67(an antigen for cell proliferation) was detected by immunohistochemistry

and the expression of ER and PR was detected by Western blot. Results Compared with the control group

the uterine coefficient

and expression of endometrium ER and PR proteins were significantly decreased in the model group(P

0.001

0.01). In Comparison with the model group

the uterine coefficient

immunoactivity of KI67

and expression of ER and PR proteins were significantly increased in both BMMSC and EA+BMMSC groups(P

0.000 1

0.001

0.01). The expression level of PR in the EA+BMMSC group was notably higher than that in the BMMSC group(P

0.05)

but no significant difference was found between the BMMSC and EA+BMMSC groups in the levels of uterine coefficient and ER expression(P

0.05). Conclusion EA combined with BMMSC injection has a positive effect in promoting the proliferation of endometrium cells in rats with thin endometrium

which may be related to its effect in up-regulating the expression of ER and PR proteins.

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Related Author

WANG Zhao-xian

XIA Liang-jun

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CUI Chu-ting

CHENG Jie

SHEN Jie

XIA You-bing

HAO Sai-nan

Related Institution

College of Acupuncture-moxibustion and Tuina & Health Preservation and Rehabilitation， Nanjing University of Chinese Medicine

Nanjing Medical University

College of Acupuncture-moxibustion and Health-rehabilitation，Nanjing University of Chinese Medicine

College of Acupuncture-Moxibustion and Tuina， Chengdu University of Traditional Chinese Medicine

The Second Clinical Medicine College， Shanxi University of Chinese Medicine

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