LU Yan, WANG Shi-jun, SONG Xiu-tao. Effects of Electroacupuncture on Glucose Transporter-1 Expression of Hippocampal Microvascular Endothelial Cells in Rats with Focal Cerebral Ischemia[J]. Acupuncture research, 2010, 35(2): 118-123.
DOI:
LU Yan, WANG Shi-jun, SONG Xiu-tao. Effects of Electroacupuncture on Glucose Transporter-1 Expression of Hippocampal Microvascular Endothelial Cells in Rats with Focal Cerebral Ischemia[J]. Acupuncture research, 2010, 35(2): 118-123. DOI: 10.13702/j.1000-0607.2010.02.007.
Effects of Electroacupuncture on Glucose Transporter-1 Expression of Hippocampal Microvascular Endothelial Cells in Rats with Focal Cerebral Ischemia
Objective To observe the effect of electroacupuncture(EA) on the expression of glucose transporter-1(GLUT-1) of hippocampal microvascular endothelial cells in focal cerebral ischemia(CI) rats.Methods Sixty male Wistar rats were randomly divided into sham-operation
model and electroacupuncture(EA) groups.CI model was established by middle cerebral artery occlusion with a piece of thread embolus.EA(4 Hz/20 Hz
1 mA) was applied to bilateral "Neiguan"(PC 6) for 30 min
once daily for 15 days.GLUT-1 expression in the hippocampus was detected with immunohistochemistry and Western blot
respectively.Results After the treatment
compared with the sham-operation group
the expression of GLUT-1 in microvascular endothelial cells of the hippocampus in model group shown by both histochemical and Western blot techniques decreased apparently(P<0.05).While in comparison with model group
the expression of GLUT-1 in EA group was upregulated significantly(P<0.05).Conclusion EA of PC 6 can effectively upregulate GLUT-1 expression in the hippocampus in cerebral ischemia rats
which may contribute to its effect in improving CI by raising the glucose transportation of microvascular endothelial cells.
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Related Author
MAO Qing-ju
Chen Bang-guo1
ZHU Tao
LI Jie
WAN Hong-ye
CHANG Bu
CHENG Yan-ting
JI Yu-fang
Related Institution
Department of Chinese Medicine,Affiliated Tianyou Hospital of Wuhan University of Science and Technology
1College of Acupuncture and Orthopedics,Hubei University of Chinese Medicine
College of Acupuncture-Moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine
The Second Clinical Medicine College, Shanxi University of Chinese Medicine
Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences