Afferent Nerve Connection among "Hegu" (LI4),"Neiguan" (PC6),"Futu" (LI18) and Thyroid Gland Region:Fluorescent Double Labelling Method

ZHANG Lu; JIANG Jin; JIN Zhi-gao; LIU Jun-ling

doi:10.13702/j.1000-0607.2010.06.008

您当前的位置：

首页 >

文章列表页 >

Afferent Nerve Connection among "Hegu" (LI4),"Neiguan" (PC6),"Futu" (LI18) and Thyroid Gland Region:Fluorescent Double Labelling Method

更新时间：2023-08-11

- Afferent Nerve Connection among "Hegu" (LI4),"Neiguan" (PC6),"Futu" (LI18) and Thyroid Gland Region:Fluorescent Double Labelling Method
- Acupuncture Research Vol. 35, Issue 6, Pages: 438-442(2010)
- 作者机构：
  
  中国中医科学院针灸研究所
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2010.06.008
  CLC：
- Published：2010
- 稿件说明：
移动端阅览
ZHANG Lu, JIANG Jin, JIN Zhi-gao, et al. Afferent Nerve Connection among "Hegu" (LI4),"Neiguan" (PC6),"Futu" (LI18) and Thyroid Gland Region:Fluorescent Double Labelling Method[J]. Acupuncture research, 2010, 35(6): 438-442.
DOI：

ZHANG Lu, JIANG Jin, JIN Zhi-gao, et al. Afferent Nerve Connection among "Hegu" (LI4),"Neiguan" (PC6),"Futu" (LI18) and Thyroid Gland Region:Fluorescent Double Labelling Method[J]. Acupuncture research, 2010, 35(6): 438-442. DOI： 10.13702/j.1000-0607.2010.06.008.

摘要

目的:观察"合谷""内关""扶突"穴区与甲状腺区之间的传入神经联系途径

为针刺该3穴区行甲状腺针麻手术机制提供形态学依据。方法:30只Wistar雄性大鼠分为合谷-扶突穴组、内关-扶突穴组、甲状腺-扶突穴组、合谷-甲状腺组、内关-甲状腺组

每组6只。分别采用荧光素碘化丙啶(PI)及双苯甲亚胺(Bb)从上述穴区皮下和甲状腺周围微量注射。PI注射后60 h、Bb注射后12 h将动物以常规方法灌流固定

剥取颈节段脊神经节及脊髓

荧光显微镜下观察PI、Bb单双标记细胞。结果:所有组中PI及Bb单标记细胞在C1-T1脊神经节均可见到

其中"合谷"穴与"内关"穴PI单标记细胞集中分布在C4-C8脊神经节

"扶突"穴与甲状腺Bb单标记细胞集中分布在C1-C6脊神经节。合谷-扶突穴组、内关-扶突穴组、合谷-甲状腺组、内关-甲状腺组双标记细胞主要分布于C3-C7脊神经节

甲状腺-扶突穴组双标记细胞分布于C1-C4脊神经节。在脊髓前角也可见少量单标记细胞

约占同一节段脊神经节全部标记细胞数的8%左右

并可见散在的双标记细胞。结论:"合谷""内关""扶突"之间及3穴位与甲状腺之间均存在双标记细胞

即上述穴位之间及穴位与甲状腺之间存在由外周到脊髓的联系途径

为这些穴位组方配伍使用在甲状腺手术针麻过程中发挥镇痛作用的机制提供了形态学依据。

Abstract

Objective To observe the peripheral and central neural connection of acupoint "Hegu"(LI4)

"Neiguan"(PC6)

"Futu"(LI18) and the thyroid gland(TG) region with fluorescence double-labelling method.Methods Thirty male Wistar rats were divided into LI4-LI18 group

PC6-LI18 group

TG-LI18 group

LI4-TG group

and PC6-TG group

with 6 rats in each.Fluorescence dyes Propidium Iodide(PI

10μL) and Bisbenzimide(Bb

10μL) were

separately

injected into those acupoints mentioned above and the TG region.Sixty hours after PI-injection and 12 hours after Bb-injection

the rats under deep anesthesia were transcardiacally perfused with PBS containing 4% polyoxymethylene

followed by taking the spinal cord and dorsal root ganglia(DRGs) of the cervical segments(C1-C8) and thoracic 1(T1) segment.Fluorescent single-and dual-labeled cells of the sliced DRGs and cervical spinal cord were observed by fluorescence microscope.Results 1) All PI-and Bb-labeled cells were found to distribute in DRGs from C1-T1 segments.PI single-labeled cells from LI4 and PC6 mainly distributed in DRGs from C4 to C8.Bb single-labeled cells from LI18 and TG region distributed in DRGs from C1-C6.2) Dual-labeled cells in the LI4-LI18 group

PC6-LI18 group

LI4-TG group

and PC6-TG group distributed in DRGs from C3 to C7

suggesting bifurcate peripheral processes of the cervical DRG neurons to innervate LI18

LI4

PC6 and the TG region.And the dual-labeled cells of the TG-LI18 group distributed mainly in DRGs from C1 to C4.3) A small number of single-labeled neurons(about 8% of total labeled cells in DRGs)and only several dual-labeled neurons were found in the anterior horn of the cervical spinal cord.Conclusion LI18

LI4 and PC6 and the thyroid gland have the same peripheral cells in DRGs of C3-C7 segments

suggesting that the bifurcate peripheral innervation may provide an anatomic evidence for the analgesic effect of acupuncture of LI18

LI4 and PC6 on thyroidectomy.

关键词

Keywords

references

Views

287

下载量

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Clinical observation on treatment of functional dyspepsia by injection of vitamin B1 into Zusanli (ST36) and Hegu (LI4)

Effect of electroacupuncture pretreatment on differential expression profiles of myocardial long non-coding RNA and mRNA in mice with myocardial ischemia-reperfusion injury

Correlation between blood oxygen level dependent fMRI signal and GABA content in anterior cingulate cortex after acupuncture of Hegu(LI4)

Observation of electroacupuncture intervention on cell apoptosis and Bax and Bcl-2 expression in cerebral and myocardial tissues in cerebral ischemia rats based on “heart-brain correlation” theory

Related Author

HUANG Xuan-bin

FU Kai-long

CHEN Jin-xiong

ZHANG Xiu-hong

ZHOU Jia

WANG Ke

FENG Ji-jie

XIE Chen-long

Related Institution

Department of Traditional Chinese Medicine, Fujian Medical University Union Hospital

Department of Traditional Chinese Medicine, The First Affiliated Hospital of Fujian Medical University

Shuguang Hospital Affiliated to the Shanghai University of Traditional Chinese Medicine

Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine

Department of Radiology,Guang'anmen Hospital of China Academy of Chinese Medical Sciences

Postal code：100700
Tel：010-64089344,84014607 Email：zcyjbjb@vip.163.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05017590号-8 京公网安备11010802020460号
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰