Effects of Electroacupuncture at"Futu"(LI 18),etc.on Expression of Spinal 5-HT 1AR mRNA,5-HT 2AR mRNA and Protein in Rats with Neck Incision Pain

QIAO Li-na; YANG Yong-sheng; WANG Jun-ying; GAO Yong-hui; HAN Yan-jing; CHEN Shu-ping; JI Chang-fu; LIU Jun-ling

doi:10.13702/j.1000-0607.2011.06.002

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Effects of Electroacupuncture at"Futu"(LI 18),etc.on Expression of Spinal 5-HT 1AR mRNA,5-HT 2AR mRNA and Protein in Rats with Neck Incision Pain

更新时间：2023-08-11

- Effects of Electroacupuncture at"Futu"(LI 18),etc.on Expression of Spinal 5-HT 1AR mRNA,5-HT 2AR mRNA and Protein in Rats with Neck Incision Pain
- Acupuncture Research Vol. 36, Issue 6, Pages: 391-396(2011)
- 作者机构：
  
  中国中医科学院针灸研究所
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2011.06.002
  CLC：
- Published：2011
- 稿件说明：
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QIAO Li-na, YANG Yong-sheng, WANG Jun-ying, et al. Effects of Electroacupuncture at"Futu"(LI 18),etc.on Expression of Spinal 5-HT 1AR mRNA,5-HT 2AR mRNA and Protein in Rats with Neck Incision Pain[J]. Acupuncture research, 2011, 36(6): 391-396.
DOI：

QIAO Li-na, YANG Yong-sheng, WANG Jun-ying, et al. Effects of Electroacupuncture at"Futu"(LI 18),etc.on Expression of Spinal 5-HT 1AR mRNA,5-HT 2AR mRNA and Protein in Rats with Neck Incision Pain[J]. Acupuncture research, 2011, 36(6): 391-396. DOI： 10.13702/j.1000-0607.2011.06.002.

摘要

目的:观察电针双侧"扶突"等穴对甲状腺区切口痛大鼠颈段脊髓中5-羟色胺(HT)受体(R)亚型5-HT 1AR和5-HT 2AR mRNA及蛋白表达的影响

探讨电针缓解颈部切口痛的可能机制。方法:Wistar雄性大鼠随机分为正常组、模型组、扶突组、合谷-内关组、足三里-阳陵泉颈段组、足三里-阳陵泉腰段组

每组8只。沿大鼠颈中线做长约1.5cm的纵行切口

制作切口痛模型。分别电针双侧"扶突""合谷"-"内关""足三里"-"阳陵泉"穴

各30min。用辐射热测大鼠切口部位热痛阈;用荧光定量Real-time PCR和免疫印迹(Western blot)方法检测颈段(C1-C4)、腰段(L1-L3)脊髓5-HT 1A和5-HT 2A受体亚型mRNA和蛋白的表达水平变化。结果:与颈部切口术前比较

各组的热辐射躲避潜伏期明显缩短(P<0.05);电针"扶突"穴或"合谷"-"内关"穴后

与本组术后比较其躲避潜伏期显著延长(P<0.05);电针"足三里"-"阳陵泉"与本组术后比较差异无统计学意义(P>0.05)。与正常组比较

模型组颈段脊髓中5-HT 1AR mRNA的表达明显升高(P<0.05)

5-HT 2AR mRNA和蛋白的表达均明显上调(P<0.05);与模型组比较

扶突组和合谷-内关组脊髓中5-HT 1AR的mRNA表达水平均明显下降(P<0.05)

5-HT 2AR的mRNA和蛋白的表达水平均明显上调(P<0.05)

足三里-阳陵泉组5-HT 1AR和5-HT 2AR的mRNA表达水平与模型组比较差异均无统计学意义(P>0.05)。结论:电针"扶突"和"合谷"-"内关"穴可缓解大鼠颈部急性切口痛

该作用可能与其下调颈段脊髓中5-HT 1AR mRNA表达、上调5-HT 2AR mRNA和蛋白的表达水平相关。

Abstract

Objective To observe the effect of electroacupuncture(EA) at bilateral "Futu"(LI18)

etc.on the expression of 5-HT1A receptor(R) mRNA

5-HT2AR mRNA and protein in the spinal cord of rats with neck incision pain

so as to explore its underlying mechanism in relieving incision pain.Methods A total of 48 Wistar rats were randomly divided into control

model(incision pain)

Futu(LI18)

Hegu(LI4)-Neiguan(PC6

LI4-PC6)

Zusanli(ST36)-Yanglingquan(GB34) cervical cord(ST36-GB34 C) and lumbar cord(ST36-GB34 L) groups.A 1.5cm longitudinal incision was made in the middle of the neck under Isoflurane inhalation anesthesia.Pain threshold(PT) was measured using radiant heat.EA(1-2mA

2Hz/15Hz) was applied to bilateral LI18

LI4-PC6

ST36-GB 34 for 30min.The expression of 5-HT1AR mRNA

5-HT2AR mRNA and protein in the cervical spinal cord(C1-C4) tissue

and 5-HT1AR mRNA and 5-HT2AR mRNA in the lumbar cord(L1-L3) were detected with Real-time PCR and Western blot analysis

respectively.Results After the operation

the thermal PT was shortened obviously in comparison with pre-operation(P<0.05).Compared with post-operation

the PT values were increased markedly in the LI18 group and LI4-PC 6 group(P<0.05)

rather than in the ST36-GB 34 group(P>0.05).The expression levels of 5-HT1AR mRNA

5-HT2AR mRNA and 5-HT2AR protein in the cervical cord of the model group were increased significantly compared with those of the control group(P<0.05).In comparison with the model group

the expression level of 5-HT1AR mRNA was down-regulated considerably in the LI18 and LI4-PC 6 groups(P<0.05)

and those of spinal 5-HT2AR mRNA and protein were up-regulated significantly in the LI18 and LI4-PC 6 groups(P<0.05).No significant changes were found in the expression of 5-HT1AR mRNA and 5-HT2AR mRNA in the ST36-GB 34 C group in comparison with the model group(P>0.05).The expression levels of 5-HT1AR mRNA and 5-HT2AR mRNA in the ST36-GB34 L group were significantly lower than those of the ST36-GB 34 C group(P<0.05).Conclusion EA of LI18 and LI4-PC 6 can significantly suppress pain reaction of neck incision in the rat

which is closely associated with its effects in down-regulating the expression of 5-HT1AR mRNA and in up-regulating 5-HT2AR mRNA and 5-HT2AR protein in the cervical spinal cord.

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