Effects of Electroacupuncture Intervention at Different Time in A Day on Expression of Tyrosine Hydroxylase and c-fos in Nucleus Accumbens in Ketamine Addiction Rats
|更新时间:2023-08-11
|
Effects of Electroacupuncture Intervention at Different Time in A Day on Expression of Tyrosine Hydroxylase and c-fos in Nucleus Accumbens in Ketamine Addiction Rats
WU Feng, XIONG Ke-ren, WANG Li-fa. Effects of Electroacupuncture Intervention at Different Time in A Day on Expression of Tyrosine Hydroxylase and c-fos in Nucleus Accumbens in Ketamine Addiction Rats[J]. Acupuncture research, 2012, 37(2): 119-124.
DOI:
WU Feng, XIONG Ke-ren, WANG Li-fa. Effects of Electroacupuncture Intervention at Different Time in A Day on Expression of Tyrosine Hydroxylase and c-fos in Nucleus Accumbens in Ketamine Addiction Rats[J]. Acupuncture research, 2012, 37(2): 119-124. DOI: 10.13702/j.1000-0607.2012.02.009.
Effects of Electroacupuncture Intervention at Different Time in A Day on Expression of Tyrosine Hydroxylase and c-fos in Nucleus Accumbens in Ketamine Addiction Rats
Objective To observe the effect of electroacupuncture(EA) intervention at different time in a day on the expression of tyrosine hydroxylase(TH) and c-fos in the Nucleus Accumbens(NAc) so as to explore its mechanism underlying improvement of ketamine addiction.Methods A total of 56 Sprague-Dawley rats were randomized into normal control
normal saline(2 mL/kg)
model and EA groups.The EA group was subdivided into Zi-Shi(23:00)
Mao-Shi(05:00)
Wu-Shi(11:00) and You-Shi(17:00) subgroups.Ketamine addiction model was established by intraperitoneal administration of ketamine(100 mg/kg)
once a day for 7 days.EA(2 Hz
3 V) was applied to unilateral "Zusanli"(ST 36) and "Sanyinjiao"(SP 6) for 30 min
once daily for 7 days.The expression of TH and c-fos in the NAc was detected by immunohistochemistry.Results Compared with the normal control group and normal saline group
the numbers of TH and c-fos immunoreaction(IR)-positive neurons in NAc in the model group were upregulated significantly(P<0.01).Compared with the model group
the expression levels of both TH and c-fos IR-positive neurons were down-regulated significantly in the Wu-Shi(11:00) and You-Shi(17:00) subgroups(P<0.01)
rather than in the Zi-Shi and Mao-Shi subgroups(P>0.05).Conculsion EA of "Zusanli"(ST 36) and "Sanyinjiao"(SP 6) at 11:00 and 17:00 of the daytime can down-regulate ketamine addiction induced increase of expression of TH and c-fos in the NAc in ketamine addiction rats
which may contribute to its effect in relieving ketamine addiction symptoms in clinic.
Effect of Electroacupuncture Intervention at Different Time-points in A Day on Expression of c-fos and Neuronal Nitric Oxide Synthase in Medial Prefrontal Cortex in Ketamine Addiction Rats
Effect of electroacupuncture on GLP-1R/PI3K/Akt protein pathway in mice with Parkinson’s disease
Effect of electroacupuncture on expression of p-ERK1/2 and c-fos in spinal dorsal horn in rats with interstitial cystitis
Electroacupuncture improves intestinal function by down-regulating expression of 5-HT_(1A)R and c-fos proteins in the hypothalamus and colon tissues via brain-gut axis in rats with functional diarrhea
Related Author
HUANG Rui
XU Ming-shu
FANG Cheng
XU Jia
ZHANG Gen-feng
GE Lin-bao~
LI Han-zhang
QI Ling
Related Institution
Shanghai Research Institute of Acu-moxibustion and Meridians
1 Shanghai Jiahua United CO.,LTD
2 Shanghai Yueyang Integrated Medicine Hospital
3 Shanghai Research Center for Acupuncture and Meridians
College of Acupuncture and Orthopaedics of Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-moxibustion