Effect of Electroacupuncture at Different Acupoints on Expression of Cervico-spinal GDNF and BDNF and Their Receptor Genes in Neck-incision Pain Rats

WANG Shao-jun; TAN Lian-hong; LIU Jun-ling

doi:10.13702/j.1000-0607.2012.05.004

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Effect of Electroacupuncture at Different Acupoints on Expression of Cervico-spinal GDNF and BDNF and Their Receptor Genes in Neck-incision Pain Rats

更新时间：2023-08-11

- Effect of Electroacupuncture at Different Acupoints on Expression of Cervico-spinal GDNF and BDNF and Their Receptor Genes in Neck-incision Pain Rats
- Acupuncture Research Vol. 37, Issue 5, Pages: 351-356(2012)
- 作者机构：
  
  中国中医科学院针灸研究所
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2012.05.004
  CLC：
- Published：2012
- 稿件说明：
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WANG Shao-jun, TAN Lian-hong, LIU Jun-ling. Effect of Electroacupuncture at Different Acupoints on Expression of Cervico-spinal GDNF and BDNF and Their Receptor Genes in Neck-incision Pain Rats[J]. Acupuncture research, 2012, 37(5): 351-356.
DOI：

WANG Shao-jun, TAN Lian-hong, LIU Jun-ling. Effect of Electroacupuncture at Different Acupoints on Expression of Cervico-spinal GDNF and BDNF and Their Receptor Genes in Neck-incision Pain Rats[J]. Acupuncture research, 2012, 37(5): 351-356. DOI： 10.13702/j.1000-0607.2012.05.004.

摘要

目的:观察电针"扶突"穴区等对颈部切口痛痛反应及颈髓胶质细胞源性神经营养因子(GDNF)等神经营养因子及其受体基因表达的影响

探讨针刺缓解颈部切口痛的机制。方法:SD雄性大鼠随机分为正常组、模型组、扶突组、内关-合谷组及足三里-阳陵泉组

每组10只。沿大鼠颈中线做长约1.5cm的纵行切口

制作切口痛模型。用辐射热在术前、术后4h及针刺治疗后测大鼠切口部位热痛阈。电针刺激上述各穴区30min后

取颈髓(C1-C4)段组织。用实时荧光定量聚合酶链反应法分析颈髓GDNF及其受体GFRα-1、脑源性神经营养因子(BDNF)及其受体TrkA、TrkB mRNA的表达。结果:颈部切口手术后各组与术前相比痛阈显著降低(P<0.05)

扶突组、内关-合谷组及足三里-阳陵泉组治疗后与本组切口术后比较

热痛阈明显增高(P<0.05)。模型组GDNF mRNA、GFRα-1mRNA的表达明显低于正常组(P<0.05)

3个电针组治疗后两个指标均明显高于模型组(P<0.001)。模型组BDNF mRNA的表达明显高于正常组(P<0.05)

TrkA mRNA、TrkB mRNA与正常组比较差异无统计学意义(P>0.05);电针各组3个指标与模型组比较差异无统计学意义(P>0.05)。结论:电针刺激能显著提高颈部切口痛大鼠痛阈

上调颈部脊髓GDNF及其受体GFRα-1基因表达的水平

表明GDNF及其受体GFRα-1参与电针对颈部切口痛的镇痛效应。

Abstract

Objective To observe the effect of electroacupuncture(EA) of "Futu"(LI 18)

etc.on the expression of genes of glial cell line-derived neurotrophic factor(GDNF)

brain-derived neurotrophic factor(BDNF) and their receptors in the cervico-spinal cord in neck-incision pain rats

so as to study its mechanism underlying incision-pain relief.Methods A total of 50 Sprague Dawley male rats were randomly divided into normal

model(incision pain)

EA-Futu(LI 18)

EA-Hegu(LI 4)-Neiguan(PC 6

LI 4-PC 6) and EA-Zusanli(ST 36)-Yanglingquan(GB 34

ST 36-GB 34) groups(n=10/group).A 1.5 cm long longitudinal incision was made along the midline of the neck under anesthesia to duplicate neck-incision pain model.Thermal pain threshold(PT) was measured before and after modeling and after the treatment

respectively.EA(1-2 mA

2 Hz/100 Hz) was applied to bilateral LI 18

PC 6-LI 4 and ST 36-GB 34 for 30 min.The expression of genes of GDNF and its receptor GFRα-1

and BDNF and its receptors TrkA

TrkB in the spinal cord(C1-C4) tissue was detected by quantitative real-time-PCR.Results In comparison with pre-modeling in the same one group

the thermal PT levels were decreased obviously in the model

LI 18

LI 4-PC 6 and ST 36-GB 34 groups after neck incision(P<0.05).Compared with the model group

the PT levels were increased markedly in LI 18

LI 4-PC 6 and ST 36-GB 34 groups after the EA treatment(P<0.05).Correspondingly

the expression levels of spinal GDNF mRNA and GFRα-1 mRNA were obviously lower

as well as BDNF mRNA was markedly higher in the model group than in the control group(P<0.05)

and those of TrkA mRNA and TrkB mRNA were increased slightly in the model group than in the control group(P>0.05).In comparison with the model group

the expression levels of spinal GDNF mRNA and GFRα-1 mRNA were up-regulated considerably(P<0.001)

whereas those of BDNF mRNA

TrkA mRNA and TrkB mRNA were decreased slightly(P>0.05) in the LI 18

LI 4-PC 6 and ST 36-GB 34 groups after EA treatment.Conclusion EA stimulation can significantly suppress pain reaction of neck incision

which is closely associated with its effects in up-regulating the expression of GDNF and its receptor GFRa-1 genes in the cervical spinal cord(C1-C4).

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