Effects of Electroacupuncture Stimulation of Scalp-point on Cardiac Sympathetic Discharges,Myocardial β_1-adrenoceptor Protein Expression and Plasma Norepinephrine Concentration in Myocardial Ischemia-reperfusion Injury Rats
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Effects of Electroacupuncture Stimulation of Scalp-point on Cardiac Sympathetic Discharges,Myocardial β_1-adrenoceptor Protein Expression and Plasma Norepinephrine Concentration in Myocardial Ischemia-reperfusion Injury Rats
LI Mei-ping, SUN Guo-jie, YOU Xing-hong. Effects of Electroacupuncture Stimulation of Scalp-point on Cardiac Sympathetic Discharges,Myocardial β_1-adrenoceptor Protein Expression and Plasma Norepinephrine Concentration in Myocardial Ischemia-reperfusion Injury Rats[J]. Acupuncture research, 2012, 37(5): 385-389.
DOI:
LI Mei-ping, SUN Guo-jie, YOU Xing-hong. Effects of Electroacupuncture Stimulation of Scalp-point on Cardiac Sympathetic Discharges,Myocardial β_1-adrenoceptor Protein Expression and Plasma Norepinephrine Concentration in Myocardial Ischemia-reperfusion Injury Rats[J]. Acupuncture research, 2012, 37(5): 385-389. DOI: 10.13702/j.1000-0607.2012.05.010.
Effects of Electroacupuncture Stimulation of Scalp-point on Cardiac Sympathetic Discharges,Myocardial β_1-adrenoceptor Protein Expression and Plasma Norepinephrine Concentration in Myocardial Ischemia-reperfusion Injury Rats
Objective To explore the mechanism of electroacupuncture(EA) of scalp-point in the management of myocardial ischemia-reperfusion injury(MI/RI) by examining its effects on left cardiac sympathetic nerve activity
myocardial β1-adrenaline receptor(AR) protein expression and plasma norepinephrine(NE) concentration in MI/RI rats.Methods Eighteen Wistar rats were randomly divided into sham
model and EA groups(n=6).MI/RI model was induced by ligation of the left anterior descending coronary artery for 30 min
followed by release of the ligation for 15 min.EA was applied to bilateral Epangxian I(MS 2) for 15 min.The left cardiac sympathetic nerve activity was recorded with BL-420 E+ biological signal acquisition system.Myocardial β1-AR protein expression was examined by western blot and plasma NE level detected by enzyme-labeled immunosorbent assay.Results Compared with the sham group
the left cardiac sympathetic discharges and plasma NE levels and myocardial β1-AR protein expression were markedly increased in the model group(P<0.01)
whereas in comparison with the model group
the sympathetic discharges
plasma NE level and myocardial β1-AR protein expression in the EA group were down-regulated significantly(P<0.05).Conclusion Scalp-point EA intervention can suppress MI/RI induced increase of sympathetic nerve activity and plasma NE level
and β1-AR protein expression
which may contribute to its effect in relieving myocardial ischemia.
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