Effect of Electroacupuncture Intervention at Different Time-points in A Day on Expression of c-fos and Neuronal Nitric Oxide Synthase in Medial Prefrontal Cortex in Ketamine Addiction Rats

WU Feng; HUANG Rui; XIONG Ke-ren

doi:10.13702/j.1000-0607.2013.05.008

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Effect of Electroacupuncture Intervention at Different Time-points in A Day on Expression of c-fos and Neuronal Nitric Oxide Synthase in Medial Prefrontal Cortex in Ketamine Addiction Rats

更新时间：2023-08-11

- Effect of Electroacupuncture Intervention at Different Time-points in A Day on Expression of c-fos and Neuronal Nitric Oxide Synthase in Medial Prefrontal Cortex in Ketamine Addiction Rats
- Acupuncture Research Vol. 38, Issue 5, Pages: 386-392(2013)
- 作者机构：
  
  皖南医学院解剖学教研室
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2013.05.008
  CLC：
- Published：2013
- 稿件说明：
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WU Feng, HUANG Rui, XIONG Ke-ren. Effect of Electroacupuncture Intervention at Different Time-points in A Day on Expression of c-fos and Neuronal Nitric Oxide Synthase in Medial Prefrontal Cortex in Ketamine Addiction Rats[J]. Acupuncture research, 2013, 38(5): 386-392.
DOI：

WU Feng, HUANG Rui, XIONG Ke-ren. Effect of Electroacupuncture Intervention at Different Time-points in A Day on Expression of c-fos and Neuronal Nitric Oxide Synthase in Medial Prefrontal Cortex in Ketamine Addiction Rats[J]. Acupuncture research, 2013, 38(5): 386-392. DOI： 10.13702/j.1000-0607.2013.05.008.

摘要

目的:观察不同时辰电针"足三里"和"三阴交"穴对氯胺酮成瘾大鼠内侧前额叶皮质(mPFC)c-fos、神经元型一氧化氮合酶(nNOS)表达的影响

探讨电针治疗氯胺酮滥用成瘾的作用机制。方法:SD大鼠随机分为正常对照组、生理盐水组、模型组、电针治疗组

电针治疗组再分为子时(23:00)、卯时(05:00)、午时(11:00)、酉时(17:00)4个亚组

每组8只。每天1次、连续7d经腹腔注射氯胺酮复制氯胺酮成瘾模型。不同时辰电针组在给药7d后分别选取一侧"足三里"和"三阴交"穴给予低频(2Hz)电针治疗

每次30min

连续治疗7d。采用免疫组织化学染色方法检测mPFC内c-fos、nNOS的表达。结果:与正常对照组和生理盐水组相比较

模型组mPFC内c-fos、nNOS免疫反应阳性神经元的表达明显增加(P<0.05)。与模型组相比较

午时、酉时电针组c-fos、nNOS免疫反应阳性神经元的表达明显减少(P<0.05);子时、卯时电针组则无明显变化(P>0.05)。结论:氯胺酮成瘾使mPFC内c-fos、nNOS的表达明显增加

可能促成神经元的适应性改变和损伤;午时、酉时电针"足三里"和"三阴交"穴可明显下调mPFC内c-fos、nNOS的表达

恢复性调节神经元的活动

修复损伤

改善氯胺酮成瘾症状。

Abstract

To observe the effects of electroacupuncture(EA)stimulation of"Zusanli"(ST 36)and"Sanyinjiao"(SP 6)at different time-points of a day on the expression of c-fos and neuronal nitric oxide synthase(nNOS)in the medial prefrontal cortex(mPFC)in rats with ketamine addiction.Methods A total of 56SD rats were randomized into control group

saline group

model group and EA group.The EA group was further divided into Zi-shi(23:00)

Mao-shi(05:00)

Wu-shi(11:00)and You-shi(17:00)subgroups(n=8in each group or subgroup).Ketamine addiction model was established by intraperitoneal administration of ketamine(100mg/kg)

once a day for 7days.EA(2Hz

3V)was applied to unilateral"Zusanli"(ST 36)and"Sanyinjiao"(SP 6)acupoints for 30min

once daily for 7days.The expression of c-fos and nNOS in the mPFC was detected by immunohistochemistry.Results Following EA intervention for 7days

the animals' daily life activities including body stretching

standing

jumping

circling

falling on the back and fur-licking in the EA group(Wu-shi and You-shi subgroups)were close to those of the normal control and saline groups

being much better than the model group.Compared with the normal control group and saline group

the expression levels of c-fos and nNOS in the mPFC in the model group were significantly upregulated(P<0.05).Compared with the model group

the expression levels of both c-fos and nNOS were considerably down-regulated in the Wu-shi(11:00)and You-shi(17:00)subgroups(P<0.05)

rather than in the Zi-shi(23:00)and Mao-shi(05:00)subgroups(P>0.05).Conclusion EA of ST 36and SP 6at 11:00and 17:00can decrease the expression of c-fos and nNOS in the mPFC in ketamine addiction rats

which may contribute to its effects in improving the rats' behavior activity.

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Related Institution

Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine

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Shanghai Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Graduade School, Shanghai University of Traditional Chinese Medicine

College of Acupuncture and Moxibustion,Shaanxi University of Chinese Medicine

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