Distribution of Calcitonin Gene Related Peptide Positive Neurofibers in Local Skin Tissues of “Neiting”(ST 44),“Zusanli”(ST 36)and“Futu”(ST 32)Regions in the Rat

CUI Jing-jing; WU Mei-ling; XU Dong-sheng; JING Xiang-hong; BAI Wan-zhu

doi:10.13702/j.1000-0607.2014.05.026

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Distribution of Calcitonin Gene Related Peptide Positive Neurofibers in Local Skin Tissues of “Neiting”(ST 44),“Zusanli”(ST 36)and“Futu”(ST 32)Regions in the Rat

更新时间：2023-08-11

- Distribution of Calcitonin Gene Related Peptide Positive Neurofibers in Local Skin Tissues of “Neiting”(ST 44),“Zusanli”(ST 36)and“Futu”(ST 32)Regions in the Rat
- Acupuncture Research Vol. 39, Issue 5, Pages: 377-381(2014)
- 作者机构：
  
  中国中医科学院针灸研究所
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2014.05.026
  CLC：
- Published：2014
- 稿件说明：
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CUI Jing-jing, WU Mei-ling, XU Dong-sheng, et al. Distribution of Calcitonin Gene Related Peptide Positive Neurofibers in Local Skin Tissues of “Neiting”(ST 44),“Zusanli”(ST 36)and“Futu”(ST 32)Regions in the Rat[J]. Acupuncture research, 2014, 39(5): 377-381.
DOI：

CUI Jing-jing, WU Mei-ling, XU Dong-sheng, et al. Distribution of Calcitonin Gene Related Peptide Positive Neurofibers in Local Skin Tissues of “Neiting”(ST 44),“Zusanli”(ST 36)and“Futu”(ST 32)Regions in the Rat[J]. Acupuncture research, 2014, 39(5): 377-381. DOI： 10.13702/j.1000-0607.2014.05.026.

摘要

目的:观察降钙素基因相关肽(CGRP)阳性表达神经纤维在大鼠后肢不同穴区局部组织中的分布特征。方法:5只正常实验大鼠用4%多聚甲醛经过心脏灌流后取下"内庭""足三里""伏兔"穴区局部皮肤组织

制成20μm厚组织切片

经CGRP荧光免疫组织化学方法染色后

用鬼笔环肽和核酸特异性荧光染料DAPI进行组织化学复染

再使用共聚焦显微镜进行观察和记录CGRP阳性表达神经纤维、鬼笔环肽标记的血管样结构以及DAPI标记的细胞核在这3个穴区的分布情况。结果:在"内庭""足三里""伏兔"穴区局部组织中看到CGRP阳性表达神经纤维分布在真皮和皮下组织

主要集中在血管样结构周围

其数量和长度以"内庭"穴区居多

"足三里"和"伏兔"穴区依次减少(P<0.01)。微细血管样结构在这3个穴区的分布也有依次减少的趋势。结论:CGRP阳性表达神经纤维是穴区局部组织中的重要成分

其数量从肢体远端穴区到近端穴区依次减少。这一形态学结果提示针刺肢体远端的穴位可能获得更强的针感。

Abstract

Objective To observe the distribution of calcitonin gene related peptide(CGRP)-positive neurofibers in local tissues of"Neiting"(ST 44)

"Zusanli"(ST 36)and"Futu"(ST 32)regions in the hind limbs of rats.Methods Five adult male SD rats were used in the present study.The local skin tissues of acupoint"Neiting"(ST 44)

"Zusanli"(ST 36)and"Futu"(ST 32)regions were dissected from the rat that underwent transcardiac perfusion with 4% paraformaldehyde

and cut into sections(20μm in thickness)

followed by staining with CGRP fluorescent immunohistochemical method and with phalloidin and DAPI

respectively.After that

CGRP-positive neurofibers of the samples were observed and recorded using a laser confocal microscope.Results CGRP-positive nerve fibers were found to distribute in the dermis and subcutaneous layers of local tissues of acupoint ST 44

ST 36 and ST 32

mainly concentrating around the vessel-like structure.The number and length of CGRP-positive nerve fibers were most in the ST 44 region

more in the ST 36 region and fewer in the ST 32 region

in turn(P<0.01).The distribution of the vessel-like structure also presented a decreasing trend in the above-mentioned three acupoint regions in turn.Conclusion CGRP-positive neurofibers are an important element in the local tissues of acupoint ST 44

ST 36 and ST 32 regions

and present a decreasing tendency in the number from the distal to the proximal part of the hind-limb

showing a similar tendency of needling sensation in the human body in which a more hypersensitivity exists in the distal part of the limb.

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CUI Jing-jing

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Related Institution

中国中医科学院针灸研究所

花王(中国)研究开发中心有限公司

Graduate School, Anhui University of Chinese Medicine

Department of Physiology,College of Integrated Traditional Chinese and Western Medicine,Anhui University of Chinese Medicine

Institute of Acupuncture and Meridian,College of Acupuncture and Massage,Anhui University of Chinese Medicine

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