Effect of Electroacupuncture Intervention on Expression of Testicular P 450 scc/17 β-HSD_3 in Rats with Partial Androgen Deficiency

REN Yi; YANG Xiao-guang; LI Xue-zhi; ZHANG Yu; WANG Ying; FU Yan

doi:10.13702/j.1000-0607.2015.01.003

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Effect of Electroacupuncture Intervention on Expression of Testicular P 450 scc/17 β-HSD_3 in Rats with Partial Androgen Deficiency

更新时间：2023-08-11

- Effect of Electroacupuncture Intervention on Expression of Testicular P 450 scc/17 β-HSD_3 in Rats with Partial Androgen Deficiency
- Acupuncture Research Vol. 40, Issue 1, Pages: 13-19(2015)
- 作者机构：
  
  1. 重庆医科大学中医药学院
  2. 重庆医科大学生物医学工程学院
  3. 重庆医科大学实验教学管理中心
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2015.01.003
  CLC：
- Published：2015
- 稿件说明：
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REN Yi, YANG Xiao-guang, LI Xue-zhi, et al. Effect of Electroacupuncture Intervention on Expression of Testicular P 450 scc/17 β-HSD_3 in Rats with Partial Androgen Deficiency[J]. Acupuncture research, 2015, 40(1): 13-19.
DOI：

REN Yi, YANG Xiao-guang, LI Xue-zhi, et al. Effect of Electroacupuncture Intervention on Expression of Testicular P 450 scc/17 β-HSD_3 in Rats with Partial Androgen Deficiency[J]. Acupuncture research, 2015, 40(1): 13-19. DOI： 10.13702/j.1000-0607.2015.01.003.

摘要

目的:观察中老年部分雄激素缺乏综合征(partial androgen deficiency of aging male

PADAM)雄性大鼠睾丸细胞色素P 450侧链裂解酶(cytochrome P 450side chain cleavage

P 450scc)及17β-羟基类固醇脱氢酶3(17β-hydroxysteroid dehydrogenase 3

17β-HSD3)的表达及电针干预对其表达的影响

探讨电针治疗PADAM的可能作用机制。方法:SD大鼠随机分为正常组、模型组和电针组

每组10只

腹腔注射环磷酰胺复制PADAM模型。电针组取"肾俞""关元"

每日治疗1次

每次15min

连续治疗8周。治疗前后观察大鼠悬尾时间和强迫游泳时间

采用酶联免疫吸附法检测大鼠血清总睾酮(total testosterone

TT)、游离睾酮(free testosterone

FT)水平

免疫组织化学法、免疫印迹法和逆转录聚合酶链反应法检测大鼠睾丸P 450scc和17β-HSD3蛋白及mRNA的表达。结果:治疗前

模型组与正常组比较

悬尾不动时间延长

强迫游泳力竭时间缩短(P<0.05);治疗后

电针组悬尾不动时间短于模型组而强迫游泳力竭时间长于模型组(P<0.01)。与正常组相比

模型组大鼠血清TT、FT水平

睾丸P 450scc和17β-HSD3蛋白和mRNA表达显著降低(P<0.01);与模型组相比

电针组血清TT、FT水平

睾丸P 450scc和17β-HSD3蛋白和mRNA表达显著升高(P<0.01)。结论:电针可能通过调节睾酮合成限速酶P 450scc和17β-HSD3蛋白和mRNA的表达

从而促进睾酮的合成和分泌。这可能是电针治疗PADAM的作用机制之一。

Abstract

Objective To observe the effect of electroacupuncture(EA)intervention on expression of cytochrome P 450 side chain cleavage(P 450scc)and 17β-hydroxysteroid dehydrogenase 3(17β-HSD3)in the testis in partial androgen deficiency of aging male(PADAM)rats so as to reveal its mechanism underlying improving PADAM.Methods Thirty male SD rats were randomly and equally divided into control

model

and EA groups.The PADAM model was established by intraperitoneal injection of cyclophosphamide(20mg·kg-1·d-1)

once daily for 5days.EA(20-30 Hz

1-3mA)was applied to bilateral"Shenshu"(BL 23)and"Guanyuan"(CV 4)for 15 min

once daily for 8weeks.Serum total testosterone(TT)and free testosterone(FT)levels were measured using enzyme-linked immunosorbent assay(ELISA).The expression levels of P 450scc/17β-HSD3 proteins and mRNA in the testis tissue were assayed by immunohistochemistry

Western bolt(WB)and RT-polymerase chain reaction(RTPCR)

separately.Results Compared with the control group

both serum TT

FT levels and expression levels of P 450scc/17β-HSD3 proteins and mRNA in the testis tissue in the model group were significantly down-regulated(P<0.01).After EA intervention

compared with the model group

the cyclophosphamide-induced decrease of serum TT

FT levels and the expression levels of P 450scc/17β-HSD3 proteins and mRNA in the testis was reversed in the EA group(P<0.01).Conclusion EA intervention is effective in up-regulating serum TT and FT

testicular P 450 scc and 17β-HSD3 proteins and mRNA levels in PADAM rats

which may be one of its mechanisms underlying improvement of PADAM.

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