Effects of Electroacupuncture on Learning-memory Ability and Expression of Hippocampal Vascular Endothelial Growth Factor(VEGF),VEGF Receptor 1 and 2 Genes in Vascular Cognitive Impairment Rats

CAI Rong-lin; CHENG Hong-liang; ZHOU Ting; CHEN Guo-qing; CHEN Xing-sheng; WU Sheng-bing

doi:10.13702/j.1000-0607.2015.01.005

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Effects of Electroacupuncture on Learning-memory Ability and Expression of Hippocampal Vascular Endothelial Growth Factor(VEGF),VEGF Receptor 1 and 2 Genes in Vascular Cognitive Impairment Rats

更新时间：2023-08-11

- Effects of Electroacupuncture on Learning-memory Ability and Expression of Hippocampal Vascular Endothelial Growth Factor(VEGF),VEGF Receptor 1 and 2 Genes in Vascular Cognitive Impairment Rats
- Acupuncture Research Vol. 40, Issue 1, Pages: 25-29(2015)
- 作者机构：
  
  1. 安徽中医药大学针灸经络研究所
  2. 安徽中医药大学附属针灸医院
  3. 安徽中医药大学科研实验中心
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2015.01.005
  CLC：
- Published：2015
- 稿件说明：
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CAI Rong-lin, CHENG Hong-liang, ZHOU Ting, et al. Effects of Electroacupuncture on Learning-memory Ability and Expression of Hippocampal Vascular Endothelial Growth Factor(VEGF),VEGF Receptor 1 and 2 Genes in Vascular Cognitive Impairment Rats[J]. Acupuncture research, 2015, 40(1): 25-29.
DOI：

CAI Rong-lin, CHENG Hong-liang, ZHOU Ting, et al. Effects of Electroacupuncture on Learning-memory Ability and Expression of Hippocampal Vascular Endothelial Growth Factor(VEGF),VEGF Receptor 1 and 2 Genes in Vascular Cognitive Impairment Rats[J]. Acupuncture research, 2015, 40(1): 25-29. DOI： 10.13702/j.1000-0607.2015.01.005.

摘要

目的:探讨电针对血管性认知障碍大鼠学习记忆及海马内血管内皮生长因子(VEGF)及其受体1(VEGFR-1/Flt-1)、受体2(VEGFR-2/Flk-1)mRNA表达的影响

为临床治疗血管性认知障碍提供理论和实验依据。方法:Wistar大鼠60只

随机选取12只作为假手术组

其余大鼠采用改良的四血管阻断法进行血管性认知障碍模型复制

将模型复制成功的大鼠保留36只

随机分为模型对照组、电针治疗组和西药治疗组

每组12只。电针治疗组大鼠电针"大椎""百会""水沟""神庭"穴

西药治疗组予茴拉西坦0.0625g/kg灌胃

均1次/d

10次为1个疗程

共治疗2个疗程。采用跳台实验测试各组大鼠学习记忆成绩

测定各组大鼠的神经行为学评分

RT-PCR法检测大鼠海马VEGF、Flt-1、Flk-1mRNA的表达。结果:模型对照组大鼠学习成绩表现为反应时间延长、错误次数增多

记忆成绩表现为潜伏时间缩短、错误次数增多

神经行为学评分显著升高

海马内VEGF、Flt-1、Flk-1 mRNA表达增强

与假手术组比较差异有统计学意义(均P<0.01);电针治疗组大鼠学习记忆成绩改善明显

神经行为学评分显著降低

海马内VEGF、Flt-1、Flk-1mRNA表达明显增强

与模型对照组比较

差异有统计学意义(均P<0.01)。电针治疗组大鼠学习成绩、神经行为学评分、海马内VEGF mRNA及Flt-1 mRNA与西药治疗组比较差异亦有统计学意义(均P<0.05)。结论:电针能显著提高血管性认知障碍大鼠学习记忆成绩

上调海马内VEGF、Flt-1、Flk-1mRNA的表达

促进了要害部位的血管生成

可能是其治疗血管性认知障碍的重要作用机制之一。

Abstract

Objective To observe the effect of electroacupuncture(EA)of"Dazhui"(GV 14)

"Baihui"(GV 20)

etc.on learning-memory ability and expression of vascular endothelial growth factor(VEGF)mRNA

VEGF receptor 1(VEGFR-1/Flt-1)mRNA

and VEGFR-2(Flk-1)mRNA in the hippocampus in vascular cognitive impairment(VCI)rats so as to reveal its mechanism underlying improvement of VCI.Methods A total of 60 Wistar rats were randomly divided into sham operation

VCI model

EA and medication groups(n=12in each group).The VCI model was established by occlusion of the bilateral vertebral arteries and bilateral cervical arteries.EA(2Hz/20Hz)was applied to"Baihui"(GV 20)

"Dazhui"(GV 14)

"Shuigou"(GV 26)and"Shenting"(GV 24)for 20 min

once daily for 20 days.Rats of the medication group were treated by intragastric perfusion of Aniracetam capsules(0.0625g/kg)

once daily for 20 days.The rats' learning-memory ability was detected by step-down test.The expression levels of VEGF mRNA

Flt-1mRNA and Flk-1mRNA in the hippocampus were detected by RT-PCR

and the neurological deficit scores were assessed by Zea Longa(0-4scaling)method.Results Compared with the sham operation group

rats of the model group showed a significant increase in reaction time and error number and decrease of escape latency(reduction of learning-memory ability)

and increase in neurological deficit score

and in expression levels of hippocampal VEGF mRNA

Flt-1mRNA and Flk-1mRNA(all P<0.01).After EA treatment

in comparison with the model group

the learning-memory ability and hippocampal VEGF mRNA

Flt-1mRNA and Flk-1mRNA were apparently increased

neurological deficit score were markedly decreased in the EA group(all P<0.01).The effects of EA treatment was obviously superior to those of medication in raising learning-memory ability and up-regulating hippocampal VEGF mRNA and Flt-1mRNA expression levels

and in reducing neurological deficit score(all P<0.05).Conclusion EA intervention can apparently up-regulate hippocampal VEGF mRNA

Flt-1mRNA and Flk-1mRNA expression in VCI rats

which may contribute to its effect in improving learning-memory ability

possibly by promoting neovasculization.

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Related Institution

School of Acupuncture-Moxibustion and Tuina, （， Beijing University of Chinese Medicine，，）

School of Chinese Medicine， Beijing University of Chinese Medicine， Beijing 100029， China）, （， Beijing University of Chinese Medicine

Graduate School of Anhui University of Chinese Medicine

The First Affiliated Hospital of Anhui University of Chinese Medicine

School of Acupunc-ture-moxibustion and Tuina,Beijing University of Chinese Medicine

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